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1

Virus-like particles of potato leafroll virus as potential carrier system for nucleic acids

100%
Suluja E., Strokowskaja L., Zagorski-Ostoja W., Palucha A.
Acta Biochimica Polonica
|
2005
|
tom 52
|
nr 3
Potato leafroll virus is a member of the polerovirus genus. The isometric virion is formed by a coat protein encapsidating single-stranded, positive-sense, mono-partite genomic RNA with covalently attached viral protein at the 5' end. The coat protein of the virus exists in two forms: i) a 23 kDa protein, the product of the coat protein gene, and ii) a 78 kDa protein, the product of the coat protein gene and an additional open reading frame expressed by read-through of the coat protein gene stop codon. The aim of this work was the expression of potato leafroll virus coat protein-based proteins that would be able to assemble into virus-like particles in insect cells. These modified particles were tested for their ability to encapsidate nucleic acids. Two types of N-terminally His-tagged coat protein constructs were used for the expression in insect cells: one, encoding a 23 kDa protein with the C-terminal amino-acid sequence corresponding to the wild type coat protein and the second with additional clathrin binding domain at the C-terminus. The expression of these two proteins by a recombinant baculovirus was characterized by Western immunoblotting with antibodies directed against potato leafroll virus. The protection or putative encapsidation of nucleic acids by these two coat protein derivatives was shown by DNase I and RNase A protection assays.
2

New inoculation techniques for potato leafroll virus

88%
Kryczynski S., Szyndel M. S.
Annals of Warsaw Agricultural University. Horticulture
|
1987
|
tom 14
3

Some methods of potato leafroll virus detection in plants

88%
Kryczynski S., Szyndel M. S.
Annals of Warsaw Agricultural University. Horticulture
|
1987
|
tom 14
4

Inhibited long distant movement of potato leafroll virus [PLRV] in some potato clones

88%
Flis B., Wasilewicz-Flis I.
Phytopathologia Polonica
|
2003
|
tom 27
5

Elements regulating Potato leafroll virus sgRNA1 translation are located within the coding sequences of the coat protein and read-through domain

63%
Loniewska-Lwowska A., Chelstowska S., Zagorski-Ostoja W., Palucha A.
Acta Biochimica Polonica
|
2009
|
tom 56
|
nr 4
619-625
 Translation of viral proteins from subgenomic RNAs (sgRNAs) is a common strategy among positive-stranded RNA viruses. Unlike host mRNA, sgRNA of Potato leafroll virus (PLRV) does not possess a cap at its 5' end nor a poly(A) tail at the 3' terminus, both of which are known to be crucial for translation of RNA in eukaryotic cells. Here, we demonstrate, that in wheat germ extract (WGE) truncation of the sgRNA1 5' UTR increases translation efficiency, as it has previously been observed in rabbit reticulocyte lysate (RRL), whereas removal of the 3' UTR does not affect translation. We also describe two regulatory elements located within the coding sequence of the coat protein (CP) gene and its read-through domain (RTD) and are responsible for regulation of in vitro translation of the PLRV sgRNA1. The first element is composed of the purine sequence AAAGGAAA located between the AUG codons of the CP and 17K genes. Deletion of this domain or its substitution by pyrimidines reduced by half the translation of both genes, whereas deletion of the RTD resulted in a 3.6-fold reduction in translation efficiency. This is the first report of translation regulatory elements of plant viruses located within a coding region.
6

Ultrastructural studies Nicotiana clevelandii plants doubly infected with potato leafroll virus and potato virus Y

63%
Garbaczewska G., Kerlan C.
Annals of Warsaw Agricultural University. Agriculture
|
2001
|
nr 39
17-26
7

Infectious transcripts from cloned cDNA of potato leafroll luteovirus

63%
Sadowy E., Pluta K., Gronenborn B., Hulanicka D.
Acta Biochimica Polonica
|
1998
|
tom 45
|
nr 2
Infectious transcripts play a key role in the research on plant viruses at the molecular level. A number of cDNA clones covering the whole genome of the Polish isolate of potato leafroll virus were constructed. Four overlapping clones were selected and assembled using restriction sites. The full copy was positioned between T7 RNA polymerase promoter and unique ScaI site. The full-length capped transcripts of the sequence of the viral genome synthesised in vitro were able to replicate in protoplasts and to produce the viral coat protein
8

Digoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid [PSTVd], potato virus Y [PVY], and potato leafroll virus /PLRV/

63%
Welnicki M., Zekanowski C., Zagorski W.
Acta Biochimica Polonica
|
1994
|
tom 41
|
nr 4
A molecular probe, p3POT, was constructed of PSTVd, PVY, PLRV cDNA fragments introduced into pUCl8 vector. Sequencing of the inserts revealed that cloned fragments covered conservative parts of pathogenic genomes. Dot-blot hybridization of digoxigenin-labelled construct to crude extracts from plants infected with different potato viruses proved high sensitivity and specificity of the p3POT probe. This makes p3POT probe an useful tool for the routine testing, and selection of virus-free potatoes
9

Studies on the translation mechanism of subgenomic RNA of potato leafroll virus

63%
Juszczuk M., Zagorski-Ostoja W., Hulanicka D. M.
Acta Biochimica Polonica
|
1997
|
tom 44
|
nr 1
The expression of open reading frames located on the subgenomic RNA (sgRNA) has been studied in an in vitro transcription and translation system. The obtained results indicate: a) translation of sgRNA occurs according to the scanning model since the insertion of a palindrome (AGo = -61 kcal/mol) pre­vents the initiation of translation; b) ORF6 is translated by suppression of the stop codon separating ORF4 from ORF6 and the presence of suppressor tRNA is necessary for the read through; c) the presence of leader sequence of sgRNA (212 nucleotides) decreases the translation efficiency of ORFs located down­stream and it afTects the ratio of products of ORF4 and ORF5; d) 3UTR does not influence on an expression of genes located on the sgRNA.
10

Zastosowanie metod molekularnych do wykrywania wirusa lisciozwoju ziemniaka [PLRV]

51%
Treder K., Lewosz J.
Progress in Plant Protection
|
2000
|
tom 40
|
nr 1
177-187
11
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Zagrozenie plantacji nasiennych ziemniaka przez wirusy Y i lisciozwoju w 2003 roku

51%
Kostiw M., Robak B.
Ziemniak Polski
|
2003
|
nr 3
27-31
12

Wplyw lokalizacji zrodel wirusow w warunkach naturalnych na rozprzestrzenianie sie PVY i PLRV

51%
Wrobel S., Turska E.
Progress in Plant Protection
|
2007
|
tom 47
|
nr 2
371-374
13

Zrodlo wysokiej odpornosci ziemniaka na wirus lisciozwoju [PLRV] i jego charakterystyka

51%
Wasilewicz-Flis I., Dziewonska M.
Biuletyn Instytutu Ziemniaka
|
1997
|
tom 48
|
nr 1
81-89
14

Mozliwosci stosowania uprawy zagonowej w nasiennictwie ziemniaka

51%
Wrobel S., Turska E.
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
|
2004
|
nr 232
269-273
15

Odpornosc rodow ziemniaka polskiej hodowli i odmian zagranicznych na wirusy Y i lisciozwoju

51%
Pietrak J.
Progress in Plant Protection
|
1997
|
tom 37
|
nr 2
330-332
16
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Prognoza porazenia sadzeniakow ziemniaka zbioru 1998 r. wirusami Y i lisciozwoju

51%
Kostiw M., Robak B., Dulinska B.
Ziemniak Polski
|
1998
|
nr 3
11-13
17
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Content available

Prognoza porazenia sadzeniakow ziemniaka zbioru 1995 roku wirusami Y i lisciozwoju

51%
Kostiw M., Woznica W., Dulinska B., Robak B.
Ziemniak Polski
|
1995
|
nr 3
40-43
18

Wplyw roznej wilgotnosci gleby na wzrost i rozwoj roslin ziemniaka oraz porazenie bulw wirusem lisciozwoju

51%
Goliszewski W.
Biuletyn Instytutu Ziemniaka
|
1994
|
nr 44
47-53
19
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Porazenie sadzeniakow ziemniaka zbioru 1994 r.wirusem Y i lisciozoju

51%
Kostiw M., Woznica W., Dulinska B., Robak B.
Ziemniak Polski
|
1994
|
nr 4
3-4
20
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Ogolna ocena zagrozenia plantacji nasiennych ziemniaka przez wirusy Y i lisciozwoju w 2001 roku

51%
Kostiw M., Robak B.
Ziemniak Polski
|
2001
|
nr 3
2-7
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