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The present investigation was carried out to evaluate the potential of chitosan alone and in combination with various agricultural wastes for the management of rootknot nematode, Meloidogyne incognita on eggplant cv. ‘BR-112’ under greenhouse conditions. The results showed that chitosan as a single or joint treatment with agricultural wastes significantly (p ≤ 0.05) reduced root-knot indices, and the nematode population in soil. As a result, of this, the growth and growth yielding attributes of eggplant were remarkably augmented. Chitosan as an elicitor induced plant mediated systemic resistance against M. incognita in eggplant. The results of the study demonstrated that maximum reduction in eggmass/root, eggs/eggmasses, nematode population and root-knot indices, was acquired by the treatments: chitosan + onion and chitosan + mentha. It was followed by chitosan + Brassica, chitosan + urad and chitosan + coconut whereas, chitosan combined with corn cob waste was found to be the least effective when compared to the control. The application of chitosan alone was effective but not very satisfactory. Compared to the control applications of all the treatments significantly increased plant growth in terms of length, fresh and dry weights, pollen fertility, yield and biochemical parameters such as chlorophyll, carotenoid content and antioxidant enzymes. This may have been due to the eliciting activity of chitosan, causing systemic resistance in the plant and the release of various toxic chemical compounds during decomposition which have lethal effects against the second stage juveniles of M. incognita and nematode multiplication.
Agricultural wastes management is a step toward sustainable agriculture. Peanut shelles as remained wastes of cultivating peanut has considerable volume which its compost can be used as available sources of ornamental plants medium. A factorial experiment based on randomized completely blocks design was conducted to investigate the impact of peanut shelles compost as the growth medium of Camellia in different concentrations of potassium. Fifteen treatments, every treatment at three replicates and 45 plots were used for experiment. The rooted cuttings of Camellia were planted and periodic watering with Hoagland solution was performed two times per week. After five months, the plants growth indices and the concentration and uptake nutrients were measured. Results showed that peanut shelles compost increased growth indices than in the control. In the most growth indices, the plant indicated a better response to 40% (w/w) compost and finally 60%. The results are indicator of providing the needed potassium for plant by peanut shelles compost than in the control.
In the pot experiments conducted on the four mountain meadow soils with high reserves of soil potassium, a possible supply of this element for ryegrass supply was examined, when potassium was excluded or its dose was limited in the fertiliser. Differences in potassium removal with yield during exhaustive cultivation were difficult to justify using both static and dynamic indicators of plant supply with potassium. Thus, dynamics of potassium transformations in the soil corresponding to the uptake amount, was assessed through the speed with which reserve potassium was passing into the solution using oxalic acid, and by the share of potassium from unexchangeable forms in the removal of this element removal with ryegrass yield.
A pot experiment was conducted in the green house to determine the effect of municipal solid waste on the growth of maize (zea mays). Growth parameters of percentage emergence, plant height, leaf area and number of levers per plant were collect and subjected into statistical analysis, using ANOVA and fisher’s L.S.D. at 5 % probability level. Plant growth parameters decreased with increase in cropping cycle. Similarly, maize grown on dumpsite soils did better than the control soil samples. It show increase in plant height, leaf area and number of leaves per plant at a range of 16.82 cm to 12.87 cm, 5 to 4 and 64.69 cm to 59.88 cm for the dumpsite and control samples respectively. Soil PH, organic matter (OM), total Nitrogen (N), Phosphorus (P), Potassium (K), Calcium (Ca), Magnesium (Mg), Sodium (Na) and Effective Cat-ion Exchange Capacity (ECEC) decreased with increase in cropping cycle respectively. The Dumpsite soil sample recorded higher mean values than the control (P < 0.05). There is every indication that municipal solid waste is beneficial to plant if only proper and careful sorting and separation of hazardous waste is done.
At present, chlorine counts as one of the elements which are essential for growth and development of plants because it plays an important role in main physiological processes as well as in plant protection against diseases. In Poland, the problem of chlorine shortage does not appear, therefore there are no studies on its present concentration and accumulation in field crops. However, the content of chlorine in plants and their tolerance to its excess in soil are varied. Under conditions of its high availability in the environment, its excessive accumulation is possible because chlorine is easily taken up from soil and directly from air. The aim of this study has been to find out the concentration and accumulation of chlorine in winter oilseed rape organs in dependence on N and Cl supply from the flowering up to the full maturity phase. The experiment was conducted in a greenhouse of IUNG-PIB, in Mitscherlich pots, where winter oilseed rape was cultivated. The first experimental factor was nitrogen fertilization (1.4 and 2.8 g pot-1) and the second was chlorine application (0, 0.47, 0.97 and 1.42 g pot-1). Chlorine concentration in plants was dependent on N dose, which caused its decrease, and on Cl dose, which resulted in its increase. The most abundant in chlorine were leaves while flowers and developing siliques contained the least of this element. Chlorine accumulated mainly in rape leaves. The accumulation of chlorine in oilseed rape increased under higher plant supply in N and Cl. The experimental plants showed a moderate supply in Cl, except the object fertilized with the highest dose of chlorine.
A pot culture was carried out with Oryza sativa L. vari-Co-39, to investigate the effects of supplementary calcium chloride on plants grown at NaCl (50mM) concentration. Treatments were: (1) Control: nutrient solution alone (C); (2) nutrient solution plus 50mM sodium chloride (NaCl); (3) nutrient solution plus 10mM calcium chloride (CaCl2); (4) nutrient solution plus 15mM calcium chloride (CaCl2); (5) nutrient solution and 50 mM NaCl plus supplementary 10 mM CaCl2 (NaCl + CaCl2); and (6) 50 mM NaCl plus additional mixture of 15 mM CaCl2 in nutrient solution (NaCl + CaCl2). The plants grown under salt stress produced low dry weight and relative water content than those grown in standard nutrient solution and in CaCl2 alone. Supplemental calcium chloride added to nutrient solution containing salt significantly improved growth and relative water content. Membrane permeability increased with high NaCl application and these increases in root membrane permeability were decreased with supplementary Ca. The concentration of chloride (Cl) increases highly for all treatments. Sodium (Na) concentration in plant tissues increased in both shoots and roots at high NaCl treatment. Application of supplementary Ca lowered Na concentration. Concentrations of Ca. K and N were at deficient ranges in the plants grown at high NaCl levels and these deficiencies were corrected by supplementary Ca. The ameliorating effect of Ca on growth and physiological variables could reduce the negative effect of salinity of Oryza sativa L., plants.
В сосудах опытах исследовали лодородие песчаной почвы содержащей ниже 10% илистых частиц взятой из под длительной залежи, черного пара и кратко- и долговременного севооборота. Применяли два уровня минерального удобрения. В сосудах был посеян овес сорта Диадем, с оставлением по 15 растений на сосуд. Установлено снижение плодородия почвы из под черного пара. Высшая масса зерна без минерального удобрения была получена на почве из под севооборота, а в условиях удобрения NPK - на почве из под залежи. Влияние удобрения на образующие урожай признаки овса было очень сильным на всех сравниваемых почвах.
Murraya koenigii L. (family: Rutaceae), commonly referred to as curry leaf, is a highly valued plant due to its aroma and medicinal features. Two dominant AM species Glomus mosseae and Acaulospora laevis were isolated from the rhizospheric soil of M. koenigii. A pot experiment was performed to verify the interactive potential of G. mosseae and A. laevis alone or in combination with Pseudomonas fluorescens on M. koenigii. Various morphological and biochemical parameters were measured after 120 days. Overall results suggest that all co-inoculation treatments showed beneficial effects on all the growth, physiological and oil content. The overall results demonstrate that the co-inoculation of bioinoculants, like P. fluorescens with AM fungi, promotes higher AM colonization and spore number enhancing the nutrient acquisition, especially phosphorus (P), improving the rhizospheric condition of soil.
The effect of sewage sludge fertilization on nitrogen and sulphur content in maize was assessed in a pot experiment conducted in 2003-2005. The experimental design comprised the following treatments in four replications on three soils: treatment without fertilizer — (0); mineral fertilization — (NPK); farmyard manure — (FYM); sewage sludge A — (SSA); a mixture of sewage sludge A with peat — (MSSA); sewage sludge B — (SSB) and a mixture of sewage sludge B with peat — (MSSB). The tests were conducted on weakly loamy sand (SI), sandy silt loam (SII) and medium silt loam (SIII), which were collected from the arable layer (0-20 cm) of ploughed land in the vicinity of Krakow. Sewage sludge which originated from two municipal mechanical and biological wastewater treatment plants, and their mixtures with peat (the materials were mixed in a 1:1 weight ratio in conversion to dry mass of organic matter) were used in the experiment. After wet mineralization of maize biomass in concentrated sulphuric acid, nitrogen was determined using Kjeldahl method in a Kjeltec II Plus apparatus. Sulphur was assessed after material mineralization in a concentrated nitric acid using the ICP—AES method in a JY 238 Ultrace apparatus. Fertilization with sewage sludge and sludge mixture with peat acted significantly better (as noted during the three-year experimental period) on maize yields than fertilization with mineral salts. In comparison with organic material and farmyard manure applied to soil, fertilization with mineral salts significantly increased nitrogen content in maize biomass. Sulphur content grew markedly in maize biomass fertilized with sewage sludge in comparison with the concentration of this element assessed in plants treated with farmyard manure. Values of the N:S ratio in aerial parts of maize from organic material treatments was within the optimal value range. The widest N:S ratio was assessed in the aerial parts and roots of maize receiving mineral fertilizers (NPK).
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Cadmium is one of the toxic heavy metals which, getting in the nutrition chain through the soil-plant system, is capable of causing harm to human health. The objective of the trial was to study the effect of cadmium load in the soil-plant system. In pot experiments carried out under greenhouse conditions, effect of increasing Cd load on different crop plants (lettuce, maize) was studied. Literature data inform on the decisive role of soil reaction in mobility of cadmium in the soil. The answer was looked for the question of the effect of cadmium treatments at uniform rates on Cd uptake by plants on two soils quite different from each other in pH value. It may be concluded that increasing Cd load can result in the decrease of biomass production of maize and lettuce, but significant differences were obtained only on very acid SN soil. The concentration of Cd in plants increased on both soils as a consequence of growing Cd treatment rates. In corresponding treatments the Cd levels in lettuce and maize plants grown on very acid SE soil were several times higher than in case of plants on the KE soil. Obtained results confirm that Cd mobility on acid soil may be much higher what is clearly reflected in cadmium uptake by plants. On acid soils the cadmium uptake by plants is more intensive, therefore this toxic element is more likely to get in the nutrition chain. Considerable differences in Cd uptake can be detected among the plant species. On the same soil at the same rate of cadmium treatment. Cd level in lettuce plants was by several times higher than in maize. The lettuce plants can accumulate cadmium in the leaves at high concentrations without any visible symptoms. In the experiments no toxicity was observed on test plants.
The efficacy of Paecilomyces lilacinus strain UP1 as biological control agent of Meloidogyne incognita attacking tomato was evaluated under screenhouse condition pot experiments. P. lilacinus was formulated on rice substrate in powder form. Root weight, gall index rating, number of galls, egg masses and nematodes per one gram root sample were determined and per cent reduction in gall number was computed. Root weight and gall index ratings were significantly higher in untreated plants than those with P. lilacinus and with the commercial fungicide Nemacur. Number of galls, nematodes and egg masses per one gram root sample were significantly reduced by the application of P. lilacinus at all levels and this was comparable with Nemacur. However, egg mass count in plants treated with the lowest concentration of the biocontrol agent was not significantly different from the uninoculated control. Per cent reduction in gall number was the highest at treatment with 7.92 × 106 spores per ml of P. lilacinus.
In the third year of a pot experiment (in a greenhouse), the carryover influence of fertilization with fresh sewage sludge and, for comparison, mineral fertilization on the content and uptake of Fe, Mn, Mo, B, Ba, Sr, As, Sn, Li, and Ti by the biomass of Miscanthus sacchariflorus grass. The yield of silver-grass biomass was evaluated on the basis of two harvests (June and December). Trace elements in the tested grass as well as in the soil (after harvest) were determined by means of the ICP-AES technique after sample combustion in a muffle furnace. Significant influence of sewage sludge fertilization on yield of silver- grass biomass was found. Grass harvested in autumn contained higher contents of some analyzed trace elements (Fe, Mn, Sr, Ba, and Ti) than that harvested in summer. The uptake of trace elements uptake by the silver-grass biomass was higher from the objects fertilized with sewage sludge than the ones receiving mineral fertilization. After three years of cultivation, the soil contained more Fe, Mn, Ba, Sr, Ti, B, and Li as compared to the content before the experiment.
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