For the first time in Poland viral examination of rainbow trout by applying cells lines (BF-2 and EPC) and ELISA tests approved in European Community Countries have shown the presence of VHS and IPN viruses existing in the country. VHS virus was isolated at 11 farms and IPN virus at 31 farms situated in northern Poland. VHS virus was isolated both in sick fish as well as in carriers and IPN virus in carriers only. The presence of VHS and IPN viruses did not appear in trout from farms in southern Poland. The experimental infection of rainbow trout demonstrated the high virulence of one of the VHS isolates. It was found that ELISA tests for VHS and IPN are very useful in detecting viruses in tissue supernatants of fish with clinical symptoms but are not as useful in detecting viruses present in cases of low titters. The results of this investigation showed that it is necessary to intensify the monitoring of the VHSV and IPNV presence in carriers and especially in fish planned to be transferred from the north to south of Poland. This should be done by using fish cell lines and immunological methods recommended in EU countries e.g. ELISA tests. The mutual co-operation of fish breeders, veterinary officers, regional fish disease laboratories and the Fish Disease Laboratory of the National Veterinary Research Institute is imperative for the effective control of fish viral diseases.
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