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The effect of progesterone on oxytocin-stimulated intracellular Ca2plus mobilisation and prostaglandin secretion in porcine endometrium

100%

Kotwica G., Oponowicz A., Kurowicka B., Franczak A., Bogacka I.

Polish Journal of Veterinary Sciences

2010

tom 13

nr 4

We have studied in the porcine endometrium the expression of oxytocin receptor (OTR) mRNA and the effect of progesterone (P₄) on oxytocin/oxytocin receptor (OT/OTR) function concerning intracellular Ca²⁺mobilisation ([Ca²⁺]i), prostaglandin F2α (PGF2α) and E2 (PGE2; PG) secretion. Tissue was taken from cyclic and early pregnant pigs (days 14-16). A higher expression of OTR mRNA (P<0.05) was observed in the endometrium of cyclic than pregnant pigs. The stimulatory (P<0.05) effect of OT (10⁻⁷ M) on [Ca²⁺]i mobilisation was noticed within 15-60 s and 30-60 s in endometrial stromal cells of cyclic and pregnant pigs, respectively. In the presence of P₄ (10⁻⁵ M) basal and OT-stimulated [Ca²⁺]i concentrations decreased in stromal cells during luteolysis and pregnancy. In stromal cells P₄ delayed mobilisation of [Ca²⁺]i in response to OT by 15 s during luteolysis and had no effect during pregnancy. In cyclic and pregnant epithelial cells OT stimulated mobilisation of [Ca²⁺]i in 45 s and 60 s, respectively. Oxytocin increased (P<0.05) PGF2α secretion during luteolysis and pregnancy and PGE2 during luteolysis from endometrial slices. Progesterone did not inhibit this stimulatory effect. During luteolysis OT increased (P<0.05) PGF2α in epithelial and stromal cells and PGE2 secretion in epithelial cells. In the presence of P₄ this effect of OT was reduced only in stromal cyclic cells (6 h culture). The presence of P₄ decreased the effect of OT on [Ca²⁺]i mobilisation only in stromal cells. We found that, in most conditions, P₄ did not inhibit the OT-stimulated secretion of PG in the porcine endometrium.

In vitro effect of peroxisome proliferator activated receptor (PPAR) ligands on prostaglandin E2 synthesis and secretion by porcine endometrium during the estrous cycle and early pregnancy

80%

Bogacka I., Bogacki M., Gaglewska M., Kurzynska A., Wasielak M.

Journal of Physiology and Pharmacology

2013

tom 64

nr 1

The effect of oxytocin on hCG action in phosphoinositide turnover in porcine myometrial smooth muscle cells

70%

Kisielewska J., Ziecik A. J.

Journal of Physiology and Pharmacology

1995

tom 46

nr 3

Ograniczanie wyników

2 Journal of Physiology and Pharmacology

1 Polish Journal of Veterinary Sciences

2 Bogacka I.

1 Bogacki M.

1 Franczak A.

1 Gaglewska M.

1 Kisielewska J.

1 Kotwica G.

1 Kurowicka B.

1 Kurzynska A.

1 Oponowicz A.

1 Wasielak M.

1 Ziecik A. J.

1 2013

1 2010

1 1995

Wyniki wyszukiwania

The effect of progesterone on oxytocin-stimulated intracellular Ca2plus mobilisation and prostaglandin secretion in porcine endometrium

In vitro effect of peroxisome proliferator activated receptor (PPAR) ligands on prostaglandin E2 synthesis and secretion by porcine endometrium during the estrous cycle and early pregnancy

The effect of oxytocin on hCG action in phosphoinositide turnover in porcine myometrial smooth muscle cells