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Our study investigated the accumulation of chromium, nickel, lead and cadmium by maize (Zea mays L.), sunflower (Helianthus annuus L.), willow (Salix x smithiana Willd.), and poplar (Populus nigra L. x P. maximowiczii), and the realtionship between the contaminants in soil and in plants. The experiment was performed in contaminated soil (former waste incineration plant) at the Hradec Králové (Czech Republic) site. Plant and soil samples were collected from three plots with different risk element contents (higher amounts of Cd, Cu, Hg, Zn, Cr, Ni, and Pb). The total and available soil metal concentrations in soil were investigated. Only a low portion of risk elements were available for plants (6% Ni, 14% Cd, 1.3 % Pb, and less than 1% of Cr). Chromium, nickel, and lead showed a similar trend to element accumulation where the highest amount was found in plant roots, higher in herbs than in trees (6.83 mg Cr·kg⁻¹, 5.04 mg Ni·kg⁻¹, and 7.76 mg Pb·kg⁻¹). The highest cadmium concentration was found in leaves of willow (1.87 mg Cd·kg⁻¹) and roots of willow (3.05 mg Cd·kg⁻¹). The correlation between the concentration of risk elements in soil and in plants was the highest in the case of lead reaching up to R= 0.89. Results also indicated that translocation of Cr, Ni, Cd, and Pb from roots to aboveground biomass of willow and poplar was low (89-98% of risk elements was retained in roots). The highest translocation from plant roots to aboveground biomass of maize and sunflower was found in the case of Cd and Pb (57 and 83% of Cd, 56 and 76% of Pb). The behaviour of these elements concerning soil and plants differed among fields with unknown history of contamination and type of contaminants.
We compared gene expression levels for enzymes of carbohydrate metabolism in the twig xylem of two Populus species with the seasonal levels of starch and soluble sugars (sucrose, glucose, and fructose) and relative levels of the enzymes. Plants of Populus deltoides Bartr. ex Marsh and P. balsamifera L., 3–4 years old, were grown outside in Lubbock, TX, USA in 43 L pots. The xylem in the middle portion of the twigs was sampled during the dormant period (November–February), at bud break (for P. balsamifera), and during the growth flush (April–July). The gene expression for ADP-glucose pyrophosphorylase (AGPase), sucrose synthase (SuSy), and sucrose-phosphate synthase (SPS) generally coincided with the levels of the carbohydrates in whose metabolism these enzymes are involved. Gene expression for AGPase and its protein levels were high when the xylem starch content was high (growing period). However, P. balsamifera maintained high AGPase levels in dormant and growing twigs, unlike P. deltoides whose dormant twigs had low AGPase and low gene expression. Compared to growing twigs, gene expression for SuSy and SPS and their protein levels were higher in dormant twigs when soluble sugar content was higher. No down-regulation of these genes appears to occur when pools of the associated carbohydrates are high. Contrary to our expectation, the gene expression for bamylase was highest in growing twigs when starch content was high. High β-amylase gene expression in growing twigs may be involved in maintaining a sufficient level of soluble sugars for growth through possibly controlling the extent of starch accumulation.
The aim of the study was to compare the effect of poplar clones Hybrid 275 and Robusta on soil cover, based on their litter fall. In 2000-2002 studies on plant litter fall and leaf decomposition were conducted at two 17-year-old poplar plantations in the Wierzbiczany forest district (Northern Poland). Both clones produced similar amounts of litter fall per tree of about 11 kg/year. Leaves were the main component of the litter fall (about 80% of total mass). Plant litter fall at the poplar plantations was rich in nutrients, lacking only phosphorus. Litter fall of the analysed poplar clones decomposed rapidly. Leaves of Hybrid 275 decomposed slightly faster than those of Robusta. Leaves of Robusta decomposed more slowly because of their lower content of nitrogen, higher content of lignin and their morphology – harder and waxier. Over the 20 month period more than 70% of the mass of Hybrid 275 leaves and 50% of the Robusta leaves decomposed. The absolute accumulation of nitrogen was observed in the decomposing leaves, along with a narrowing of the C/N ratio and a more rapid decrease in potassium content than the loss of mass. Based on the properties of litter fall, we can conclude that both poplar clones would have a favourable effect on soil cover.
Habitat edges are regarded as important components of heterogeneous landscapes. Diverse theories exist about the diversity and functional role of edges, and no generalisation have been possible so far, thus case studies are important for better understanding the landscape scale processes. Forest management highly modified the structure and tree species composition of the European forests. The sylvicultural intensification resulted in the rise of the proportion of non-native, intensively managed forest stands. In the present study we explore the response of spider and ant assemblages to forest stand type and the edge effect between native poplar and non-native conifer plantations in Hungary. We applied pitfall traps to sample the ground-dwelling spiders and ants. Four plots consisting of the two forest types and the transition zones between them were selected. Five transects for each replicated plot were sampled. We identified the significant indicator species of the different habitat types. We found significant differences in the species richness (i.e. number of species) of ants and spiders of the different habitat types. We detected intermediate spider species richness at the edge indicating that edges separate a higher quality habitat from one that has lower resource quality; however, the species richness of ants was the highest at the edge and did not differ between the two forest types. The positive impacts of edge was found due to presence of generalist and grassland species at the edge and presumably edges separate patches that provide complementary resources also increasing the number of ant species.Our results indicate that forest type affects the species compositions of ground-dwelling spiders and ants. Our study also shows that habitat type had a major effect on the species richness and composition of spider and ant assemblages, suggesting that local forestry management plays a crucial role in preserving the native invertebrate fauna of forests.
In vivo and in vitro self-pollination of whole pistils of some clones of Salix viminalis enabled to obtain mature seeds containing cotyledonary embryos which after the transfer to MS medium developed into wholly formed seedlings. Pollination in vitro of placentae led to abundant pollen germination and formation of tubes which occasionally they were entering the ovules through micropyle. Fertilized ovules normally developed into germinable seeds. Distant pollination of stigmas in vivo and in vitro with pollen grains of Populus tremula, P.tomentosa, P. lasiocarpa showed the ability of pollen to germinate and to form tubes several hours after pollination. Some tubes penetrated the styles but did not enter into the placenta. When placentae were directly pollinated than pollen germinated abundantly and occasionally pollen tubes were found entering the micropyle. Embryological analysis of those ovules performed 3-5 days after pollination demonstrated the presence of globular embryos with several endosperm nuclei. The technique of in vitro placental pollination works well for Salix viminalis and it could probably be applied to other Salix species.
Adventitious bud cultures were established by using buds of selected poplar clones (Populus tremula L. × P. alba L.) as initial explants. The Murashige and Skoog medium (1/2 and 1/4 MS) was used for multiplication and rooting of shoots. To the media, aluminium was added in the form of sulphate, at a concentration 50−70 mg Al dm-3. The culture was continued in vitro for more than 12 months. The cultures developed on media with aluminium (Al+) were more tolerant to aluminium in the medium during multiplication than those developed on media without aluminium (Al−). Rooted poplar cuttings obtained from cultures on media with aluminium (Al+) grew better in soil from an area polluted by a phosphate fertilizer factory (Luboń) than those from media without aluminium (Al−). This soil was characterized by a high Al level, low Ca/Al ratio and low pH, as compared to the control soil, from an area regarded as free from toxic pollution.
The purpose of this study was to assess the influence of SO2 on photosynthetic apparatus and the level of total nonstructural carbohydrates (TNC) in developing and mature leaves of poplar (Populus deltoides). Photosynthetic apparatus was evaluated on the basis of fluorescence parameters (Fv/Fm, OPSII, qP and Rfd) and photosynthetic pigments (chlorophylls and carotenoids). Cuttings of poplar were exposed to 0.25 ppm of SO2 at 25°C and 200-300 mmol m-2s-1 PAR for 6 hours daily during 5 days in a fumigation chamber. The fumigation did not produce any significant differences in fluorescence parameters in neither developing nor mature leaves. In some mature leaves the concentration of pigments increased under the influence of SO2. Soluble carbohydrates decreased significantly both in developing and mature leaves and this was accompanied with an increase in starch accumulation. We suggest that Populus deltoides is a species tolerant to sulphur dioxide and the used SO2 dosage did not significantly impair the light reactions of photosynthesis, but it disturbed the accumulation of starch and the utilization of soluble carbohydrates in plants exposed to SO2.
Riparian forests provide important roosting habitat, abundant prey and access to drinking water for many bat species but to date there has been little research on the differential quality of habitats within riparian areas. We quantified the density of potential roost cavities in three age classes (i.e., young: ca. 20 years, mature: ca. 60 years, and old: ca. 100 years) of riparian cottonwood (Populus deltoides) forest stands. Bat activity was also sampled using acoustic detectors in one representative stand of each age class. Stands were situated along an 80 km stretch of the Missouri River in southeastern South Dakota and northwestern Iowa, USA. We predicted the highest density of potential roosts and the highest activity of bats to occur in the oldest age class. Contrary to our predictions, and previous work in aspen dominated upland sites, we found that the density of potential roosts was not significantly different between mature and old stands. However, there were no potential roosts in young stands. Data from guano traps verified the use of a number of cavities in both mature and old stands. Both commuting and foraging activities were highest in the mature, relative to the old and young stand. In total, our data indicate that mature and old stands represent high quality roosting habitat, with the mature being used preferentially for commuting and foraging. Trees in the oldest stands, however, are nearing the end of their lifespan and falling. Younger cohorts must therefore be retained for future recruitment of natural cavities.
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