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Chemical composition and cytotoxic activity of the polysaccharide fraction in Sarcodon imbricatus (Basidiomycota)

100%

Sulkowska-Ziaja K., Muszynska B., Ekiert H.

Acta Mycologica

2012

tom 47

nr 1

The aim of the study was chemical analysis of polysaccharide fractions from sporocarps of Sarcodon imbricatus collected in natural sites and from the mycelium of in vitro cultures. Three polysaccharide fractions (FOI, FOII, FOIII) were isolated from sporocarps and two (FKI, FKII) from in vitro cultures. Qualitative analysis by HPLC method showed that they are composed of galactose and fucose (FOI, FKI) or glucose and fucose (FOII, FKII). FOIII fraction of the sporocarps consisted of glucose only. Molecular weights of isolated fractions ranged from 3.8 to 16.3 kDa for fractions from the sporocarps and from 5.8 to 14.7 kDa for that ones isolated from in vitro culture. The total percentage of sugar content for all fractions ranged from 97.8% to 99.1%. The percentage of uronic acids contents in acidic fractions was 2.6% and 2.7% for the FOI and FKI respectively. The work included also an assessment of cytotoxic activity of polysaccharide fractions in relation to tumor cell lines of human breast cancer MCV-7. FOI polysaccharide fraction of the sporocarps inhibited the growth of cancer cells in 50% compared to the control at a concentration of 0.0125%, while the polysaccharide fraction FKI from in vitro cultures inhibited cell growth in a concentration of 0.016%.

Comparison of the biological activity of crude polysaccharide fractions obtained from Cedrela sinensis using different extraction methods

100%

Oh M. H., Yoon K. Y.

Polish Journal of Food and Nutrition Sciences

2018

tom 68

nr 4

Four preparations of crude polysaccharides were obtained from Cedrela sinensis using four methods of extraction, and finally precipitation with ethanol. Hot-water and processes assisted by ultrasound and enzymes (Shearzyme or Viscozyme) were carried out. The yield of polysaccharide fraction extracted with the enzyme-assisted method using Shearzyme (P-EAS) was the highest at 10.53%. All crude saccharides were composed of six monosaccharides including arabinose, galactose, glucose, mannose, rhamnose, and xylose. Crude polysaccharide fraction extracted by hot water showed the highest DPPH• and ABTS•+ scavenging activities as well as α-amylase inhibitory activity. All fractions showed higher retardation effects on glucose and bile acid absorption using the in vitro dialysis system compared to the control (without polysaccharide fraction); specifically, the glucose retardation index of P-EAS was similar to that of carboxymethylcellulose. Overall, crude polysaccharide fractions obtained from C. sinensis can be used as functional ingredients owing to their excellent functional properties.

Ograniczanie wyników

1 Acta Mycologica

1 Polish Journal of Food and Nutrition Sciences

1 Ekiert H.

1 Muszynska B.

1 Oh M. H.

1 Sulkowska-Ziaja K.

1 Yoon K. Y.

1 2018

1 2012

Wyniki wyszukiwania

Chemical composition and cytotoxic activity of the polysaccharide fraction in Sarcodon imbricatus (Basidiomycota)

Comparison of the biological activity of crude polysaccharide fractions obtained from Cedrela sinensis using different extraction methods