Wyniki wyszukiwania

1

Polyploidization of synergids in Allium angulosum L.

100%

Luszczek D., Bohdanowicz J.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2000

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tom 42

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nr 1

2

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In vitro polyploidization of Ajuga reptans L. using oryzalin

88%

Sveracova M., Navratilova B., Ondrej V.

Acta Biologica Cracoviensia. Series Botanica

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2018

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tom 60

|

nr 2

3

Low temperature induces polyploidization in dividing Amoeba proteus

75%

Afon'kin S. Y., Kalinina L. V.

Acta Protozoologica

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1987

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tom 26

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nr 2

4

Endosperm culture of Actinidia species

75%

Machno D., Przywara L.

Acta Biologica Cracoviensia. Series Botanica

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1997

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tom 39

Callus was successfully induced from the mature endosperm of three Actinidia species: A. arguta, A. deliciosa var. deliciosa (kiwifruit) and A. kolomikta. For the initiation of callus, MS medium supplemented with 2,4-D (2 or 4 mg/l) and kinetin (5 mg/l) was used. Transfer of the callus to medium containing IAA (0.1 mg/l) and BAP (1 mg/l) resulted in the formation of roots in approximately 40% of the endosperm explants of A. deliciosa. The callus, initially yellow-white, turned green when cultured in the light. In A. arguta and A. kolomikta no morphogenic response was observed on this medium. If the cultures were inoculated onto medium with IAA (0.3 mg/l) and 2iP (5 mg/l), in the endosperm calluses of kiwifruit, shoots were formed in addition to roots. In A. arguta a few abnormal shoots were produced in one explant. The sub-culture of A. arguta callus on MS without hormone evoked the production of some roots. No morphogenic response was observed in the endosperm cultures of A. kolomikta on all media tested. The counting of chromosomes in five roots and young leaves of one shoot of A. deliciosa revealed that they were triploids with chromosome number 2n = ~250.

5

Polyploidization of the suspensor in Triglochin maritimum L. [Juncaginaceae]

75%

Dabrowska D., Bohdanowicz J.

Acta Biologica Cracoviensia. Series Botanica et Zoologia. Supplement

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1997

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tom 39

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nr 1

6

Polyploidization in the suspensor of Sophora japonica L. [Fabaceae]

75%

Biskup A., Izmailow R.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2000

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tom 42

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nr 1

7

The development of embryo sac and mechanism of polyploidization in the antipodals of Epimedium pubigerum DC

75%

Unal M., Yildirim C., Ekal S.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1997

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tom 45

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nr 2-4

8

Endosperm development in seeds of Echium vulgare L. [Boraginaceae] from polluted sites

63%

Biskup A., Izmailow R.

Acta Biologica Cracoviensia. Series Botanica

|

2004

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tom 46

39-44

Analysis of endosperm development in plants from contaminated sites (vicinity of the Żelazny Most copper post-flotation reservoir in the Legnica-Głogów Copper District, and the zinc spoil in Katowice-Wełnowiec) showed general similarities in the pattern of endosperm formation in Echium vulgare, but also deviation from typical haustorium structure (~23% frequency), premature degeneration of the haustorium, or degeneration both of the haustorium and endosperm proper (~40%). The most significant irregularities of endosperm development included lower or higher number of nuclei in two cells of the lateral part of the endosperm, lower ploidy level of haustorium nuclei, and cellularization (instead of coenocytic structure) in the lateral cells of the endosperm. Irregularities and degenerative processes presumably resulted from the stress of environment conditions. Because of the nutritive function of those structures, degeneration or atypical structure of the endosperm and its haustorium in some maturing seeds may reduce the fertility of plants colonizing contaminated sites.

9

Genomic catastrophism and the origin of vertebrate immunity

63%

Hughes A. L.

Archivum Immunologiae et Therapiae Experimentalis

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1999

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tom 47

|

nr 6

10

The use of in vitro culture for studying cytological differentiation of endosperm tissue

63%

Musial K., Przywara L.

Folia Histochemica et Cytobiologica. Supplement

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1997

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tom 35

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nr 2

11

Polyploidization in the suspensor of Triglochin palustre l. [Juncaginaceae]

63%

Kozieradzka-Kiszkurno M., Swierczynska J., Bohdanowicz J.

Acta Biologica Cracoviensia. Series Botanica

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2002

|

tom 44

Differentiation of the suspensor basal cell was studied in Triglochin palustre (2n = 24). The zygote divides into the smaller apical cell and the bigger basal cell, which becomes the basal cell of the suspensor. The nuclear DNA content of the suspensor basal cell attains a high degree of ploidy, up to 256C. Nuclei with the highest ploidy levels of 128C and 256C were observed in mature basal cells (from 50- to 500-celled embryos). As a result of polyploidization the volume of the nucleus increased and changes in the chromatin structure of polyploid nuclei were noted. Endochromocenters at middle ploidy level and bundle-like aggregations of chromatin at the highest ploidy levels were found. Rhythmic enlargement of the DNA content and nuclear volume of the basal cell, as well as the characteristic structure of its chromatin, point to endoreduplication as the mechanism of polyploidization in the suspensor.

12

Cytological processes during embryo sac development in Allium angulosum L.

63%

Dabrowska D.

Acta Biologica Cracoviensia. Series Botanica. Supplement

|

1999

|

tom 41

|

nr 1

13

Biotechnology in haploids and polyploids

63%

Niemirowicz-Szczytt K.

Biotechnologia

|

1996

|

nr 1

14

Sedum acre embryogenesis: polyploidization in the suspensor

63%

Kozieradzka-Kiszkurno M., Bohdanowicz J.

Acta Biologica Cracoviensia. Series Botanica

|

2003

|

tom 45

|

nr 2

Cytological processes of differentiation in the embryo suspensor of Sedum acre L. were compared with the development of the embryo proper. The zygote undergoes an asymmetric division to produce an apical cell and a basal cell, which becomes the basal cell of the suspensor. The mature differentiated suspensor consists of a large haustorial basal cell and 3-4 chalazal cells. The basal cell nucleus gradually grows to a considerable size, and the amount of nuclear DNA also increases. The highest degree of ploidy (1024C) was observed in basal cells in large >100-celled embryos. Chromocenters at low (8C-16C) and middle (32C-64C) levels of ploidy, and endochromocenters at higher (128C-256C) and the highest (512C-1024C) levels of ploidy were observed. Changes in DNA content, nucleus size and chromatin structure point to endoreduplication as the mechanism of polyploidization of the suspensor in Sedum acre.

15

Polyploidization of endosperm chalazal haustorium of Rhinanthus serotinus [Scrophulariaceae]

63%

Swierczynska J., Kozieradzka-Kiszkurno M., Bohdanowicz J.

Acta Biologica Cracoviensia. Series Botanica

|

2005

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tom 47

|

nr 1

The paper reports a study of karyological differentiation of the chalazal endosperm haustorium of Rhinanthus serotinus (Schönheit) Oborny. Polyploidization began soon after the formation of the haustorium cell. The fully differentiated chalazal haustorium is a large elongated cell containing two huge nuclei. The nuclei are situated halfway along the length of the haustorium cell. Measurements of their nuclear DNA content revealed its degree of ploidy, which could attain a maximum 768C. Nuclei with higher (192C-384C) and the highest (768C) levels of ploidy were found in mature chalazal haustorium cells (from 100- to 500-celled endosperm proper). During polyploidization, the volume of the nuclei increased, and changes in the chromatin structure of polyploid nuclei were noted. With increasing levels of ploidy, polytene chromosomes were observed in haustorium nuclei. The rhythmical increase of DNA content and changes in nuclei size and chromatin structure point to endoreduplication as the mechanism of polyploidization of the haustorium cell.

16

Polyploidization in the amoeboid anther tapetum of Calla palustris L. [Araceae]

63%

Deregowska R., Turala-Szybowska K.

Acta Biologica Cracoviensia. Series Botanica

|

1996

|

tom 38

In Calla palustris L. (Araceae) the anther tapetum (AT) of the amoeboid type occurs. Till the stage of I telophase in pollen mother cells (PMCs), the AT of this species has a cellular character. In premeiotic stage and during 1 meiotic division in PMCs, the differentiation of the AT is accompanied by two mitotic divisions which do not occur in all tapetal cells (TCs). The first mitosis may be acytokinetic resulting in the formation of a number of binucleate TCs. The nuclei of ca 40% TCs remain on the diploid level. Polyploidization of the nuclei in uni- and binucleate TCs is mainly connected with inhibition of mitoses at prophase. As a result, in the majority of the TCs the polyploid nuclei of regular shape arise. The polyploidization may be due also to nuclear restitution or fusion of chromosome groups at metaphase and anaphase which leads to the formation of nuclei of irregular outlines. The dissolution of the cell walls in the TCs begins already at the end of I prophase in PMCs. The fusion of the protoplasts is accomplished at the tetrad stage and the periplasmodium intruding the anther loculus is formed. At the tetrad stage in the AT of C. palustris three volume classes of the nuclei corresponding to the diploid, tetraploid and octoploid levels were distinguished.

17

Adventitious shoot regeneration from leaf explants of Malus domestica Borkh. in vitro

51%

Podwyszynska M., Sowik I.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

|

tom 96

|

nr 1

18

Chromosome numbers and karyotypes of Allium przewalskianum populations

51%

Ao C.

Acta Biologica Cracoviensia. Series Botanica

|

2008

|

tom 50

|

nr 1

In this study, somatic chromosome counts were determined in 302 individuals from 43 Allium przewalskianum populations; 90 were diploids (2n = 16, 16 + 1B, 16 + 2B) and 212 were tetraploids (2n = 32). Of the 43 populations, five were selected for karyotype analysis. Among them, the diploid plants have two karyotypes: 2n = 2x = 14m + 2st (2SAT) and 2n =2x = 8m + 6sm + 2st (2SAT). The tetraploid has one: 2n = 4x = 28m + 4st. Mixoploidy, Robertsonian translations, and B chromosomes were reported for the first time. In combination with previous chromosome data, the present study reveals a uniform basic chromosomal number (x = 8) and uniformity of karyotypes (Stebbins's 2A type), indicating that speciation through polyploidization is less likely in A. przewalskianum, despite the highly diversified habitats it occupies.

19

Effect of polyploidization on survival of sea, brook, and rainbow trout hybrids during incubation and early feeding period

51%

Dobosz S., Goryczko K.

Acta Ichthyologica et Piscatoria

|

1988

|

tom 18

|

nr 2

20

Polyploidization of stigmatic papillae in Triglochin maritimum L. [Juncaginaceae]

51%

Bohdanowicz J., Dabrowska D.

Acta Biologica Cracoviensia. Series Botanica

|

1997

|

tom 39

The cytological differentiation of the dry papillate stigma in Triglochin maritimum L. (Juncaginaceae) was studied. The polyploidization process started soon after the formation of unicellular stigmatic papillae. Later, huge, long papillae with single enlarged nuclei constituted the receptive surface of the maturing stigma. The nuclear DNA content of the polyploid papillae and of telophasic (2C) and prophasic (4C) cells of the ovule was measured cytophotometrically after Feulgen staining. Analysis of nuclear DNA content measurements permitted the degrees of ploidy reached by the papillae to be established. Nuclei with DNA content corresponding to levels of 4C, 8C, 16C, 32C and 64C were found in the mature stigma. The most common were nuclei with DNA content of 16C (29%) and 32C (24%). The absence of mitoses, rhythmical enlargement of the DNA content of the nuclei as well as their characteristic endochromocenters, pointed to endoreduplication as the mechanism of polyploidization of the stigmatic papillae.

Ograniczanie wyników

Polyploidization of synergids in Allium angulosum L.

In vitro polyploidization of Ajuga reptans L. using oryzalin

Low temperature induces polyploidization in dividing Amoeba proteus

Endosperm culture of Actinidia species

Polyploidization of the suspensor in Triglochin maritimum L. [Juncaginaceae]

Polyploidization in the suspensor of Sophora japonica L. [Fabaceae]

The development of embryo sac and mechanism of polyploidization in the antipodals of Epimedium pubigerum DC

Endosperm development in seeds of Echium vulgare L. [Boraginaceae] from polluted sites

Genomic catastrophism and the origin of vertebrate immunity

The use of in vitro culture for studying cytological differentiation of endosperm tissue

Polyploidization in the suspensor of Triglochin palustre l. [Juncaginaceae]

Cytological processes during embryo sac development in Allium angulosum L.

Biotechnology in haploids and polyploids

Sedum acre embryogenesis: polyploidization in the suspensor

Polyploidization of endosperm chalazal haustorium of Rhinanthus serotinus [Scrophulariaceae]

Polyploidization in the amoeboid anther tapetum of Calla palustris L. [Araceae]

Adventitious shoot regeneration from leaf explants of Malus domestica Borkh. in vitro

Chromosome numbers and karyotypes of Allium przewalskianum populations

Effect of polyploidization on survival of sea, brook, and rainbow trout hybrids during incubation and early feeding period

Polyploidization of stigmatic papillae in Triglochin maritimum L. [Juncaginaceae]