Agrocybe aegerita is a delicious mushroom which was known in Ancient Greece and Rome. The species under natural conditions is met in Mediterranean countries as well as in the Middle and North Europe. The Agrocybe is cultivated mostly on wood or sawdust. Woods of poplar and willow are the best for cultivation of this mushroom, but logs of elm, ash-tree and elder can be useful substrate as well. The study determined the suitability of some substrates to A. aegerita cultivation. Beech, alder, oak, maple and birch sawdust and sawdust of apple-tree, pear- tree and cherry-tree were used in the experiment. Moreover substrates of rape, wheat, rye and barley straw were tested, too. The experiments showed that the yielding of A. aegerita differed significantly in relation to the kind of substrate. Beech sawdust proved to be the most suitable substrate for cultivation of this species.