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An organic-mineral fertilizer obtained from brown coal, the Rekulter when applied to polluted soil increased the content of the total organic carbon and pHKCl. The content of Cd and Zn soluble in 1M HCl were approximately 80% and for Ni and Pb were about 90% of the total content. The content of soluble Zn, Pb, Cd and Ni in 0.01 CaCl2 decreased when the application rate of the Rekulter was increased. The Rekulter reduced the content of cadmium soluble in 0.01 CaCl2 on average by 62% and nickel by 81% compared to pots not fertilized with this preparation. In the case of lead the decrease was about 2% and for zinc, about 35%.
Soil microorganisms may be both sensitive and resilient to various disturbances. The effects of a single stressor on soil microorganisms have been well studied, but only limited research has been carried out to test the effects of simultaneous action of diverse stressors. Soil samples were collected from a long-term polluted zinc and lead site and an unpolluted site. Modeling studies assumed spiking soils with five different concentrations of nickel (400, 800, 1.600, 3.200, and 6.400 mg Ni·kg⁻¹ dry weight soil) and their incubation under different humidity conditions (10%, 75%, and 120% of water holding capacity). We wanted to test if additional environmental disturbances have a different effect on microorganisms from polluted and unpolluted soils. The study showed that after 30 and 120 days of incubation, increasing Ni pollution inhibited microbial respiration rate (R), both in unpolluted and long-term metal polluted soils, irrespective of soil moisture. After 30 days of the experiment, microbial communities in both soils demonstrated a similar response to the additional toxicant. However, after 120 days of exposure to Ni, microbial communities from the unpolluted soil showed much higher inhibition of R than microbes from the polluted soils (p<0.001). The results might suggest that Ni co-tolerance mechanisms occurred in long-term metal polluted microbial communities.
This paper describes the application of two direct and one indirect methods for the extraction of microbial community DNA from soils polluted with heavy metals. DNA was extracted directly from soil by a gentle method based on the soil incubation at 37°C with proteinase K and SDS or the method was modified by the addition of bead beating step. The indirect approach was based on the RNA/DNA extraction method. The level of soil contamination did not affect on the yields of DNA extracted and PCR amplification of the target DNA. The results indicated that the DNA obtained by the applied protocols was sufficiently pure for further molecular analyses.
Activity of the enzymes can give information on the kind and duration of the effects of pollutants on the metabolic activity of soil. Results of studies on the dehydrogenase activity in soils experimentally contaminated with diesel fuel, with and without modification used in investigation over biodégradation process (including fertilization, stirring and inoculation) were presented in paper. Studies with two rates of pollution were carried out for soils of various organic matter content and different soil particle size grading. Dehydrogenase activity was determined with TTC (2,3,5-triphenyltetrazolium chloride) as a substrate. Increase of dehydrogenase activity was found in the soils that were treated with diesel fuel. Regardless of pollution rate, usually higher activity was recorded in sandy soil. Variance analysis confirmed the highly significant influence of applied modifications on increase of the enzymatic activity in relation to not modified object.
A method of determination of high Zn and Pb concentrations by means of EDXRF (energy-dispersive X-ray fluorescence spectrometry) is presented. Zn and Pb concentration in soil samples from contaminated areas ranged within a limit of Zn: 800–2000ppm and Pb: 200–600ppm. Soil analysis was conducted directly after the samples had been dried and powdered, as well as in situ in polluted areas. Due to the absence of the certificate reference materials for soils with such high Zn and Pb concentrations, samples of soil with the Pb and Zn amounts determined using the AAS method were used to perform calibration. The obtained detection limits are 30ppm and 19ppm for Zn and Pb, respectively. Because of the high analytes concentration and material heterogeneity, the repeatability of the results was examined according to the sample holder’s various positions in EDXRF analyzer; repeatability the of the results while putting into the sample holder and out of it, together with the repeatability of making the calibration. The results obtained using the EDXRF method were compared to these obtained using AAS and wavelength-dispersive X-ray fluorescence spectrometry (WDXRF).
Soil, the main element of natural environment is constantly contaminated and degraded by various man’s activities. Big industrial factories, which emil to the atmosphere excessive quantities of harmful solid, liquid and gaseous substances, arc the main cause of this phenomenon. Improper usage of fertilisers and chemical plant protection agents effects the soil in a lesser degree, but it is still unfavourable. The aim of this research was to use potato beetle larva in stage L₄ as a soil bioindicator from the plantations of spring wheat, potatoes, and corn which were treated with herbicides. The obtained results did not show any negative effect of herbicides used on potato beetle larva growing in the soil.
Accumulation of Cd, Pb, Zn, Cu (HM) by spring barley (Hordeum vulgare L.) from sod podzolic sandy loam and chernozem soils, impacted by heavy metals pollution in the soils, is studied in the article. The aim of study has been to determine spring barley bioaccumulation capacity impacted by the HM pollution with the high level of Cd, Pb, Zn, Cu concentration in soils. The HM concentration diapason caused biomass reduction – the scope of toxic tolerance was obtained. The range of contaminants concentration in soil, which caused the plant biomass reduction, from the beginning to plants death – the scope of toxic tolerance, is the index of a species reaction on selected xenobiotic. It shows: “threshold” concentration of a contaminant that caused a plant biomass inhibition; toxic process development and the correlation between contaminants concentration in soil and/or plant and the plants inhibition; the concentration that caused the plant death. Spring barley accumulation indexes of the studied metals were calculated. Relevant scopes of the plant-uptake index for each metal were calculated. Dynamics of the toxic process development of spring barley as impacted by the pollution in the break-down by studied metals were observed on two different soils. Toxic process dynamic evaluation gives the possibility to simulate concentration of the trace metal in plants, concentration of available forms of these elements in soils, and also contamination level (content of metals) that caused plants height and plant weight reduction by 10%, 50% and 90%.
The present study aimed to isolate and identify the chlorpyrifos resistant soil bacteria from contaminated soils in order to be used for bioremediation of polluted environments. Bacteria were isolated from two cultivated plant root rhizopheric soil of Cocks comb (Celosia cristata) and Marigold (Tagetes erecta).The pesticide was tested at 5 elevated doses,0.5%, 1%, 2%, 2.5% and control. Physiochemical properties of soils, PH, Electrical conductivity, Organic carbon, Organic matter, Nitrogen, phosphorus, potassium, Manganese and Iron were analyzed. Based on morphological and biochemical tests the isolates were identified as Bacillus subtilis, Pseudomonas putida, Pseudomonas aeruginosa. Thus, the organisms can be exploited for bioremediation of chlorpyrifos polluted soil, and their ability to degrade other organophosphates pesticide.
The most rational methods to reduce Cu and Zn mobility in the soil as well as their toxicity to spring triticale and pea-oat mixture cultivated on the sward-podzolic light loamy soil appeared to be double-rate liming and combined application of organic and lime fertilizers. On the soil highly contaminated with Cu and Zn it is desirable to give preference to the cultivation of annual grasses as they accumulate fewer of these elements as compared to grain crops.
The bioindicative research conducted in 1998 concerned the soil polluted by lead in the region of Glass Factory in Krosno. Potato beetle larvae descending to soil for pupation were the soil bioindicator. Samples of soil were collected in the distance of 500 to 1,000 m from the factory emitters, and later the lead content was determined in the Chemical Agricultural Station in Rzeszów. The lead level in the 1st pollution zone was 200.0 mg Pb/kg while in the 2nd zone 70.0 mg/kg, and in the soil sample from the control field the level was 12.0 mg/kg. The obtained results of the analysis conducted in the laboratory of the Regional Experimental Station did not show any significant differences between both the beetle mass which emerged from the soil, and their number from the control field and the experimental one.
The studies conducted from 1997 to 1999 in a vegetation hall were performed as a pot experiment on ordinary silt with cation exchange capacity (CEC) of 81.1 cmol (+) ˇ kg–1, pHKCl = 6.0 and organic C content of 9.5% serving as soil. Jerusalem artichoke, maize, Sida hermaphrodita Rusby, amaranth and hemp were used as indicator plants. The results confirmed, implied earlier, great diversification of the element contents which depends not only on the species but also on the part of plants. Analysis of the data revealed also another dependence: increased concentration of heavy metals in the soil corresponds to higher content of heavy metals in the plants. Significant differences were observed for the plant species from unpolluted and contaminated at various levels treatments.
W pracy określono neutralizujące działania kompostu, zeolitu i tlenku wapnia na skutki wpływu nadmiaru chromu na aktywność biochemiczną gleby. Badania prowadzono w warunkach wazonowych. Przedmiotem badań był piasek słabogliniasty zanieczyszczony chromem w ilości 0, 25, 50, 100 i 150 mg·kg⁻¹ s.m. gleby. Rośliną testową był owies. W próbkach gleby określono aktywność: dehydrogenaz, ureazy, fosfatazy kwaśnej, fosfatazy alkalicznej, katalazy i arylosulfatazy. Zanieczyszczenie gleby chromem różnicowało aktywność biochemiczną gleby. W serii bez substancji łagodzących, chrom(VI) miał bardziej negatywny wpływ na aktywność enzymatyczną gleby niż chrom(III). Wyjątkiem była aktywność fosfatazy alkalicznej, w przypadku której stwierdzono zależności odwrotne. Spośród wszystkich badanych enzymów, największe ujemne działanie obserwowano w aktywności dehydrogenaz i arylosulfatazy. Zastosowanie substancji, które miały na celu zniwelowanie działania chromu, spełniły oczekiwane nadzieje, przy czym najbardziej skutecznym okazał się kompost.
Celem niniejszej pracy było określenie wpływu zanieczyszczenia gleby niklem na tle zróżnicowanego wapnowania i nawożenia organicznego na aktywność wybranych enzymów glebowych - ureazy, fosfatazy kwaśnej i alkalicznej oraz zawartość węgla w związkach organicznych, azotu całkowitego i pH glebie po uprawie kupkówki pospolitej. Badano glebę po trzyletnim doświadczeniu wazonowym, które przeprowadzono w obiektach Akademii Podlaskiej w Siedlcach, w układzie całkowicie losowym, w trzech powtórzeniach. W doświadczeniu uwzględniono następujące czynniki: I - wapnowanie (bez wapnowania i wapnowanie wg 1 Hh gleby w formie CaCO3); II - nawożenie organiczne (O i osad ściekowy pochodzący z oczyszczalni ścieków w Siedlcach w dawce wprowadzającej do gleby 2 g C∙kg-1 gleby); III - zróżnicowane zanieczyszczenie gleby niklem (bez stosowania niklu, 50 i 100 mg Ni∙kg-1 gleby poprzez stosowanie NiCl2-6 H2O). Utwór glebowy użyty w doświadczeniu był pobierany w warstwie ornej 0-20 cm gleby płowej o składzie granulometrycznym piasku gliniastego mocnego. Rośliną testową była trawa - kupkówka pospolita (Dactylis glomerata L.), której w każdym sezonie wegetacyjnym zebrano po cztery pokosy. Analizie poddano glebę po każdym pokosie rośliny testowej. Wszystkie badane w doświadczeniu czynniki różnicowały istotnie analizowane cechy, przy czym ich wpływ zależał zarówno od cechy jak i terminu pobrania prób do analizy. Zastosowane wapnowanie spowodowało zmniejszenie aktywności fosfatazy kwaśnej, zawartości węgla organicznego i azotu całkowitego w badanej glebie oraz zwiększenie aktywności fosfatazy alkalicznej i pH. Na obiektach, na których zastosowano osad ściekowy stwierdzono większą zawartość węgla i azotu oraz aktywność wszystkich enzymów w stosunku do obiektów, na których nawożenia organicznego nie stosowano. Wzrastające ilości niklu w glebie nie różnicowały w sposób jednoznaczny badanych cech.
W doświadczeniach laboratoryjnych badano wpływ zanieczyszczenia gleby metalami ciężkimi na aktywność dehydrogenaz. Czynnikami zmiennymi w doświadczeniu były: 1) rodzaj utworu glebowego: piasek gliniasty, glina piaszczysta i glina piaszczysto-ilasta; 2) pH gleby: 5,5 oraz 7,0; 3) rodzaj metalu ciężkiego: cynk, miedź, nikiel i kadm; 4) stopień zanieczyszczenia metalami ciężkimi: zawartość naturalna - I°, zawartość podwyższona - IIº, średnie zanieczyszczenie - IIIº, silne zanieczyszczenie - IVº, bardzo silne zanieczyszczenie - Vº, 2-krotnie przekraczające bardzo silne zanieczyszczenie - VIº; 5) czas inkubacji gleby: 30, 60, 120 dni. Próbki gleb, w 3 powtórzeniach, inkubowano w temperaturze 25°C i po upływie określonego czasu inkubacji oznaczono aktywność dehydrogenaz. W wyniku badań wykazano jednoznacznie negatywny wpływ wszystkich metali ciężkich na aktywność dehydrogenaz, przy czym najsilniejszymi inhibitorami tych enzymów, we wszystkich utworach glebowych, była miedź i kadm. Dowodzą tego najniższe wartości ED₅₀ dla tych metali. Z reguły we wszystkich glebach kwaśnych wartość ED₅₀ dla cynku, miedzi i kadmu były mniejsze niż w glebach o odczynie obojętnym. W przypadku niklu taką zależność stwierdzono tylko w glinie piaszczystej.
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