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This study was carried out to determine storage ability of strawberry pollen at different temperatures for three different strawberry cultivars 'Aliso', 'Brio', and 'Cruz' . Strawberry pollen was stored at room temperature (22 ± 2 oC), +4 0C, -4 0C and -18 0C in stabile humidity conditions. Strawberry pollen was germinated using the hanging drop method in a 20% sucrose solution. Pollen germination rate increased because of low temperature storage. Pollen stored at room temperature and +4 0C, -4 0C, and -18 0C was kept for 8 months, about one year, and 20 months, respectively. Pollen germination rates decreased as the length of storage period increased. The reaction of all cultivars tested on the duration and temperature of storage was similar.
In the investigated material, consisting of 143 honey samples, pollen grains of 109 taxa were identified; 80 were represented by nectariferous plants and 29 by non-nectariferous plants. In the pollen of nectariferous plants, 17 anemophilous and 12 entomophilous taxa were found. In particular honey samples, from 1 up to 13 taxa were noted. The identified pollen grains came from plants belonging to 19 botanical families. The most frequently represented families were as follows: Rosaceae, Poaceae and Ranunculaceae. The percentage proportion of pollen of non-nectariferous taxa varied and it was within a range of 0.3% to 69.4%. The highest average frequency among anemophilous plants was demonstrated by the pollen of Poaceae (others), Quercus and Rumex, whereas among entomophilous plants by the pollen of Filipendula, Plantago and Fragaria.
Immunogold labelling revealed the presence of lipoxygenase (LOX) in different parts and types of anther cells of Gagea lutea. LOX was found in the cytoplasm and close to ER elements in epidermal and endothecial cells, and close to the cell walls of the latter. The positive immunoreaction to LOX was less intense in the middle layers and the loculus of the anther, where single immunogold particles were concentrated at the cell walls of these layers and in the protoplast masses, in vacuoles, close to mitochondria, inside plastids, and in the liquid of the anther cavity. LOX occurred in the cytoplasm and around ER elements of pollen grains as well as in the exine layer, particularly in contact regions between the outer and inner exine layers. The correlations between LOX localization in different anther cells and the functioning of particular anther parts are discussed.
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