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В случаях тотального выпадения влагалища у коров, автор предлагает применять непрерывную крестцово — влагалищную лигатуру, фиксирующую обе боковые и дорсальную стенки. Для операции следует применять низкий надоболочечный наркоз над Spotium ischiadicum maius и модифицированную иглу Герляха.
Similar bacterial floras are present in the vagina and uterus of healthy bitches. The most common of these bacteria are b-hemolytic streptococci, Pasteurella multocida and E. coli and Staphylococcus intermedius. Bacterial flora varies during the estrous cycle with the highest bacterial count occurring during heat. E. coli was most frequently isolated from bitches with pyometra. No specific bacterial species are associated with vaginitis and infertility.
The aim of this study was to compare the bacterial flora of canine vagina and uterus and assess its composition during the estrus cycle. From 28 healthy bitches, vaginal smears and samples of uterine secretion by means of endoscopy were collected during the estrus cycle (anestrus, proestrus, estrus, metestrus) for bacteriological investigation. Similar bacterial flora were present in the vagina and uterus of bitches. Lactobacillus sp.,α-hemolytic streptococci, Staphylococcus intermedius, Pasteurella multocida and E. coli were most frequently found. The average number of isolates was 2.2 per vaginal sample and 1.4 per uterine sample, the uterine samples also showed a lower bacterial count. The composition of vaginal and uterine bacterial flora and amounts of bacteria varied during the estrus cycle. The highest bacterial count occurred during proestrus.
Analysis of sIgA concentrations in the contents of the cervical canal of the uterus and of the oral cavily in women with Candida or without fungi in ontocenoses of these organs. The aim of the study was to search for fungi in ontocenoses of genital organs and oral cavity (the fungal reservoir for multifocal infections) in women; evaluation of the concentration of sIgA in the contents of the cervical canal of the uterus and of the oral cavity. 102 women (age: 18- 35 years) were examined. Fungi were isolated from ontocenoses of the vagina and the oral cavity; axenic strains were differentiated with API 20 C and API 20 C AUX tests (bioMerieux). The concentrations of sIgA in the content of the cervical canal of the uterus and from the oral cavity were evaluated by LC-Partigen IgA (Behring) tests. Candida occurence in the oral cavity was significant (p<0,02) higher than in the vagina. Candida albicans (6 codes) was the predominat species; there were also C. tropicalis, C. kefyr, C. krusei, C. guilliermondii and C. glabrata. There were no significant differences between sIgA concentrations and the presence or absence of fungi in the vagina or oral cavity.
Relationship between activity of hydrolases in fungi isolated from vagina of pregnant women and selected symptoms. Pregnancy is considered a factor of vulvovaginal mycosis. Secretion of hydrolases is an important determinant of Candida virulence. Thus, the aim of the study was to found the relationsbip between activity of 19 hydrolases in fungi isolated from vagina of pregnant women and symptoms of mycosis. 251 pregnant women were examined. Samples were collected from vagina and cultured on Sabouraud media. Activity of hydrolytic enzymes was evaluated using API ZYM (bioMerieux) test. Fungi were found in 20.1% of vaginal samples. Symptoms were detected in 45.8% of wornen. Only 32.1% of women with discharge and 26.4% with pruritus had mycosis. Out of the 19 examined hydrolazes, 13 active enzymes were detected in fungal strains. We found for the first time the relationsbip between activity of fungal esterase lipase and the presence of vaginal discharge in pregnant women.
Current data about fungi prevalence in various ontocenoses in women during procreation period. The aim of study was to analyse fungi prevalence in three ontocenoses (vagina, oral cavity and anus) in women during procreation period, which should be helpful in developing prophylactic programmes for pregnanl womem, newborns and infants infected with fungi. In 716 examined women (age 20-45 yares), 1411 samples of appropriate biological materials were taken and inoculated in the fluid Sabouraud medium and then axenic fungi strains were isolated in Sabouraud agar. High fungi prevalence in three ontocenoses of examined women was noted. Mean percentage for ontocenosis was: 39% ± 1.8% for vagina, 42.18% ± 1.8% for oral cavity and 25.03 ± 1.6% for anus. These data indicate the necessity for further examinations of multifocal fungal infection in women during procreation period.
Celem pracy było określenie udziału grzybów drożdżopodobnych z rodzaju Candida i pałeczek Enterobacteriaceae w wymazach z pochwy u kobiet. Wśród grzybów z rodzaju Candida najczęściej występowały gatunki C. albicans, C. krusei i C. tropicalis, a wśród pałeczek z rodziny Enterobacteriaceae E. coli i P. mirabilis. Grzyby z rodzaju Candida występowały najczęściej w połączeniu z E. coli.
The studies on the influence of bacteria and parvovirus infection on reproductive failure in silver and blue foxes were carried out on 21 Polish farms. Parvoviral antibodies were detected in 35 out of 229 sera tested. Parvovirus infection could be the main cause of small litter size in only 6 farms An abundant growth of E. coli, Staphylococcus aureus, Streptococcus pyogenes, Proteus vulgaris and Clostridium perfringens was demonstrated in vaginal and prepucal swabs collected from animals tested. Isolated bacteria were susceptible to norfloxacin and amoxicillin. Antibiotic treatment, acording to results of in vitro tests, of all animals before reproduction and all vixens before parturition, resulted in a decreased number of „empty vixens”, abortions and neonatal deaths. Application of a proper antibiotic or elaboration of an empiric scheme of infections for foxes would eliminate reproductive losses on fox farms.
Определено степень инфекции органа размножения кoров микроорганизмами, принадлежащими к родам Мусорlasma и Ureaplasma. Этих возбудителей прoбoзли изолировать из мазков, взятых из влагалища 136 корoв, у которых отмечались клинические симптомы, определяемые как гранулярный вулыновагикит, а также от 15 коров и 11 телок без этих симптомов. Из проведенных исследований вытекает, что коровы с симптомами гран. вулъвовагинита в высоком проценте инфицированы были микроорганизмами Ureaplasma. Этот процент колебался в заьисимости от стадa, от 53,3 до 80% мивотных. От здоровых же животных возбудителей удалось изолироавать только от 13,3% коров и 14,4% телок. Дополнительные исследования указывают на то, что на слизистй оболочке короз с симптомами гран, вульвовагинита было большее намножение уреаплазм чем у здоровых животных. Инфекция коров микоплазмами была значительно меньше и составляла 0—12,5% животных.
Bakterie z rodzaju Lactobacillus wytwarzające nadtlenek wodoru dominują w populacjach bakterii zdrowej pochwy. W pracy podjęto próbę określenia, które z gatunków Lactobacillus wykazują taką właściwość, a ponadto wyznaczono stężenia bójcze zarówno czystego chemicznie nadtlenku wodoru jak i supernatantów pochodzących z hodowli Lactobacillus wobec bakterii i grzybów kolonizujących drogi rodne kobiet.
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