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Disruption of Trichoderma reesei gene encoding protein O-mannosyl-transferase I results in a decrease of the enzyme activity and alteration of cell wall composition

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Gorka-Niec W., Pniewski M., Kania A., Perlinska-Lenart U., Palamarczyk G., Kruszewska J. S.

Acta Biochimica Polonica

2008

tom 55

nr 2

251-259

In fungi transfer of the first mannosyl residue to proteins during their O-glycosylation is catalyzed by protein O-mannosyltransferases encoded by pmt genes. Disruption of the pmt1gene in Trichodermacaused a significant decrease in the total activity of protein O-mannosyltransferases. Moreover, disruption of the pmt1gene also led to osmotic sensitivity of the strain, indicating an essential role of the PMTI protein activity for cell wall synthesis. At the same time, the strain was defective in septa formation, producing only half the number of septa per unit length of hypha compared with the wild type. Disruption of the pmt1gene decreased protein secretion but had no effect on glycosylation of secreted proteins, which suggests that PMTI protein O-mannosyltranferase does not take part in glycosylation of these proteins.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Gorka-Niec W.

1 Kania A.

1 Kruszewska J. S.

1 Palamarczyk G.

1 Perlinska-Lenart U.

1 Pniewski M.

1 2008

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Disruption of Trichoderma reesei gene encoding protein O-mannosyl-transferase I results in a decrease of the enzyme activity and alteration of cell wall composition