Wyniki wyszukiwania

1

Inhibition of collagen-induced platelet reactivity by DGEA peptide

100%

Luzak B., Golanski J., Rozalski M., Boncler M. A., Watala C.

Acta Biochimica Polonica

|

2003

|

tom 50

|

nr 4

Direct interactions between collagen, the most thrombogenic component of the extracellular matrix, and platelet surface membrane receptors mediate platelet adhesion and induce platelet activation and aggregation. In this process two glyco- proteins are crucial: integrin a2β1, an adhesive receptor, and GPVI, which is especially responsible for signal transduction. Specific antagonists of the collagen receptors are useful tools for investigating the complexity of platelet-collagen interactions. In this work we assessed the usefulness of DGEA peptide (Asp-Gly-Glu-Ala), the shortest collagen type I-derived motif recognised by the collagen-binding integrin a2β1, as a potential antagonist of collagen receptors. We examined platelet function using several methods including platelet adhesion under static conditions, platelet function analyser PFA-100™, whole blood electric impedance aggregometry (WBEA) and flow cytometry. We found that DGEA significantly inhibited adhesion, aggregation and release reaction of collagen activated blood platelets. The inhibitory effect of DGEA on static platelet adhesion reached sub-maximal values at millimolar inhibitor concentrations, whereas the specific blocker of a2β1 — monoclonal antibodies Gi9, when used at saturating concentrations, had only a moderate inhibitory effect on platelet adhesion. Considering that 25-30% of total collagen binding to is specific, we conclude that DGEA is a strong antagonist interfering with a variety of collagen-platelet interactions, and it can be recognised not only by the primary platelet adhesion receptor but also by other collagen receptors.

2

Association between microvesicles bearing monomeric C-reactive protein and platelet reactivity. Relationship with low response to antiplatelet drugs?

84%

Siennicka A.

Journal of Physiology and Pharmacology

|

2021

|

tom 72

|

nr 1

3

Effect of cis-platinum [II] amine phosphonate complexes on in vitro platelets reactivity

84%

Kostka B., Sikora J., Aranowska K., Para J., Ochocki J.

Acta Toxicologica

|

2005

|

tom 13

|

nr 2

4

Variable gender-dependent platelet responses to combined antiplatelet therapy, in patients with stable coronary-artery disease

67%

Jastrzebska M., Marcinowska Z., Oledzki S., Chelstowski K., Siennicka A., Klysz M., Clark J. S.

Journal of Physiology and Pharmacology

|

2018

|

tom 69

|

nr 4

Ograniczanie wyników

2 Journal of Physiology and Pharmacology

1 Acta Biochimica Polonica

1 Acta Toxicologica

2 Siennicka A.

1 Aranowska K.

1 Boncler M. A.

1 Chelstowski K.

1 Clark J. S.

1 Golanski J.

1 Jastrzebska M.

1 Klysz M.

1 Kostka B.

1 Luzak B.

1 Marcinowska Z.

1 Ochocki J.

1 Oledzki S.

1 Para J.

1 Rozalski M.

1 Sikora J.

1 Watala C.

1 2021

1 2018

1 2005

1 2003

Inhibition of collagen-induced platelet reactivity by DGEA peptide

Association between microvesicles bearing monomeric C-reactive protein and platelet reactivity. Relationship with low response to antiplatelet drugs?

Effect of cis-platinum [II] amine phosphonate complexes on in vitro platelets reactivity

Variable gender-dependent platelet responses to combined antiplatelet therapy, in patients with stable coronary-artery disease