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The effect of ketotifen on inflammation in the intestine during T. spiralis infection in BALB/c mice was examined. The lack of enteropathy in treated mice was accompanied to affected the size of worms and the number of worms, but did not prevent the retardation of nematodes in the intestine. Fecundity of females in vitro and the number of muscle larvae were not statistically changed after treatment. Ketotifen reduced the number of mast cells and IgA plasma cells in the intestine. As inflammatory influx, epithelial damage and IgA response are related to the action of T cells, ketotifen affected T dependent reactions in the gut of T. spiralis infected mice.
The influence of Cyclosporin A (CyA) on the course of the muscular phase of trichinellosis in mice was investigated. The animals were infected with 200 Trichinella spiralis larvae and were treated with CyA between the 14th-18th day after infection. The drug was administered at a dose of 50 mg/kg/day. An increase in the number of eosinophils and В lymphocytes was observed relative to the control group. Our results indicate that CyA elicited the secretion of Th2 cell cytokines. At the same time more granulomas and a lower number of muscle larvae were observed in mice treated with CyA.
The effect of anti-IL-5 monoclonal antibodies (mAb) on the level of eosinophilia, the number of IgA plasma cells in lamina propria and the level of IL-5 in scraped mucosa of small intestine in BALB/c mice was examined. Eosinophils and plasma cells in lamina propria were assessed in frozen sections of small intestine. The level of IL-5 was monitored using capture ELISA. The influence of mAb anti-IL-5 on the total cell number and number of eosinophils was measured in the peritoneal cavity. Intraperitoneal injection of 0.5 mg anti-IL-5 mAb on the 3rd and 5th day after infection (DAI) with 200 T. spiralis larvae strongly depressed both eosinophils and IgA plasma cells response in the lamina propria on day 15th and 24th after infection. There was a positive correlation between the number of eosinophils and the level of IL-5 as well as between the number of IgA plasma cells and level of IL-5. IL-5 was essential for eosinophils and IgA response in small intestine and a partial role for IL-5 in protection against T. spiralis was observed.
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