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Mannose inhibition of nitrate uptake by wheat roots

86%
The effect of mannose on nitrate uptake was investigated by a new non-invasive technique. Under normal condition, nitrate uptake by wheat (Triticum aestivum L.) roots was about 1-7 µmol gfwt-1 h-1. After the addition of 10 mM mannose, net nitrate influx by wheat roots started to decrease and reached a new steady state at -40 ±50% of the control value after 73 min. After mannose supplied for 2 h, its removal caused net nitrate influx to be recovered to an original rate. Therefore, the inhibition of mannose on nitrate uptake is not due to the onset of programmed cell death because it starts too rapidly and it is reversible, however, it is probably due to Pi and consequent ATP depletion.
Rye plants were grown in a nutrient solution prepa- red according to Hoagland for 2 weeks at pH 7, next for 14 days at pH 4.5 (without Cu+2) and in the presence of 20, 50, or 100 mg dm-3 copper ions. The control plants were grown continuously at pH 7. The physicochemical surface properties of the roots were exa- mined using two adsorbates – polar (water vapour) and non-polar (nitrogen). The surface properties of the roots grown at pH 4.5 without Cu+2 were apparently the same as those of controls. The roots of rye which grew in the presence of Cu+2 were characte- rized by lower (relative to controls) specific surface area values. Statistically significant differences in the size of the apparent surface area (determined by water vapour) were reported for roots in- cubated with copper ions at a concentration of 20 and 50 mg dm-3. The average water vapour adsorption energy of the root surface decreased under the stress conditions. There were no statistically significant differences for the free surface area and characteristic energy of nitrogen adsorption.
Pot experiments were designed to test the applicability of root electrical capacitance measurement for in situ monitoring of root water uptake activity by growing cucumber and bean cultivars in a growth chamber. Half of the plants were inoculated with Funneliformis mosseae arbuscular mycorrhizal fungi, while the other half served as non-infected controls. Root electrical capacitance and daily transpiration were monitored during the whole plant ontogeny. Phenology-dependent changes of daily transpiration (related to root water uptake) and root electrical capacitance proved to be similar as they showed upward trends from seedling emergence to the beginning of flowering stage, and thereafter decreased continuously during fruit setting. A few days after arbuscular mycorrhizal fungi-colonization, daily transpiration and root electrical capacitance of infected plants became significantly higher than those of non-infected counterparts, and the relative increment of the measured parameters was greater for the more highly mycorrhizal-dependent bean cultivar compared to that of cucumber. Arbuscular mycorrhizal fungi colonization caused 29 and 69% relative increment in shoot dry mass for cucumbers and beans, respectively. Mycorrhization resulted in 37% increase in root dry mass for beans, but no significant difference was observed for cucumbers. Results indicate the potential of root electrical capacitance measurements for monitoring the changes and differences of root water uptake rate.
Tolerance of several strains of the P-, S-, and F- intersterility groups of Heterobasidion annosum to low pH and high concentration of aluminium ions was studied on malt extract agar. There were marked intraspecific, but not intergroup, variations in tolerance to both factors. Some strains were relatively tolerant or sensitive to low pH and aluminium, while the others were tolerant to low pH but sensitive to Al. There was no relationship between tolerance of the strains to low pH and their tolerance to aluminium stress.
Research on soil microorganisms associated with plant roots (endophytes) and their outside habitats (rhizosphere, soil) under exposure to high concentrations of heavy metals in the environment may contribute to an increased knowledge about the effects of unfavorable pollutants in the natural ecosystem and improve the efficiency of remediation processes of degraded soils. The main goal of our study was to evaluate properties of microbial populations inhabiting different but spatially closed habitats: interior of the tree roots – endophytes (Betula pendula L. and Alnus glutinosa L.), rhizosphere and soil not influenced by plant roots at two heavy metal polluted areas in southern Poland where the content of cadmium, lead and zinc ranged 9.2–14.9, 26.2–75.1 and 1080–1559 mg∙kg–1 of dried soil, respectively. In our experiment, we analyzed their density as well as determined the total metabolic activity and ability for utilization of different carbon sources. The density of endophytes (5.76–7.16 log10 c.f.u. × g dw–1) was higher than the density of bacteria inhabiting outside habitats – the rhizosphere and soil (5.43–5.91 and 5.08–5.57 log10 c.f.u. × g dw–1, respectively) and was not affected by heavy metal content. However, the total metabolic activity of endophytic populations was strongly influenced by the physicochemical properties of the soil and was significantly lower at more contaminated site. In general, the metabolic activity of endophytes was ~15 times lower than the activity of rhizobacteria and at the same level or lower than the activity of soil microorganisms. Discriminant analysis revealed a higher utilization of α-Cyclodextrin and D-Xylose by microorganisms isolated from more polluted test site. The use of an artificial neural network (ANN) followed by principal component analysis (PCA) allowed the distinguishing of preferences in the utilization of carbon sources by microorganisms due to tree species but not the zone of isolation – endophytes, rhizosphere, soil. Our study revealed no significant impact of the metal concentrations present in the soil on the density of microorganisms (bacteria and fungi) inhabiting three analyzed zones (interior of the roots, rhziosphere, soil); however, their total metabolic activity was decreased in more contaminated site. Since the density of endophytes was higher compared to the number of bacteria present in the outside habitats (rhizosphere and soil), and their total metabolic activity was in general lower, we suggest a high specialization of microorganisms associated with the cellular spaces of plant roots compared to other microbial populations.
Zucchini is a very valuable vegetable, easy to grow both in the field and under covers but it is often attacked by soil-borne fungi. The investigations were carried out in a farm near Lublin where three zucchini cultivars: Astra, Atena and Soraya, were grown in a tunnel and in the field in 2008-2009. Plants with stem and root rot were collected for laboratory tests. Mycological analysis showed that the predominant fungi were F. culmorum, F. equiseti and F. oxysporum. More Fusarium colonies were isolated from the roots of zucchini grown in the tunnel. In the pathogenicity test, all tested isolates of F. culmorum, F. equiseti and F. oxysporum proved to be pathogenic to zucchini seedlings, causing stunting, stem and root rot. The highest disease index was noticed in the combination with F. culmorum isolate fck61.
The process of the host-plant recognition by rhizobia is complex and multi- step. The interaction between legumes and microorganisms results in the induction of the root nodule. This symbiotic interaction is highly host-specific. Bacteria within nodules fix atmospheric nitrogen. This process is of immense ecological and economic significance. The subject of this presentation is the molecular mechanism by which the bacterium determines its host-specific characteristics. First flavonoids secreted by the plant roots induce the tran­scription of bacterial genes involved in nodulation, the so-called nod genes. This leads to the next step of the signalling system, i.e. the production and secretion of lipo-oligosaccharide molecules by rhizobia. These signal molecules have various discernible effects on the roots of the host leguminous plants. The bacterial nodulation factors were isolated and structurally identified as substi­tuted and N-acylated chitin oligosaccharides. These prokaryotic signals play a key role in the symbiosis by controlling the host specificity of the bacteria. They constitute a new class of signalling molecules able to elicit nodule organogene­sis in leguminous plants in the absence of bacteria. More recent studies impli­cate involvement of root cell membrane depolarization and ion selective chan­nels in the communication processes that initiate nodule formation.
This article provides morphological and molecular characteristics of Punctodera storiei Brzeski, 1998. Comparison of partial sequences of 18S and 28S rDNA genes from P. stonei sampled in Poland and Punctodera sp. from Canada showed their 100% similarity. This is the first report on the occurrence of P. stonei outside of Europe. We provide data on morphology of males and 2nd stage juveniles of this species and an identification key to males of the genus Punctodera Mulvey et Stone, 1976. Moreover, the paper presents evolutionary relationships of P. stonei within the family Heteroderidae.
Rhodiola Kirilowii (Regel) Maxim. (Crassulaceae) is a traditional medicinal plant used in North Asia and China, especially in the cardiopulmonary disorders in the hypoxic conditions induced by high altitude. The presented results are the part of the investigations carried out in the Branch of Medicinal Plants of the Institute of Natural Fibres and Medicinal Plants in cooperation with the Department of Biology and Pharmaceutical Botany, Medical University in Warsaw on R. Kirilowii plants and tissue cultures. The aim of recent study was to determine the growth dynamics and active compounds production during the cultivation of callus tissues of R. Kirilowii on solid/liquid media. Tissue cultures of R. Kirilowii shown the ability to produce all the active compounds determined in the roots of plants of Polish origin. It is worth emphasizing, that rosavins, according to known literature, were not detected in roots of plants growing in Asia. The best time for collection the tissues from solid medium was fifth or sixth week of the culture – the tissues were growing dynamically and the contents of the main active compounds was high. The material from suspension should be collected in 12–15 days after inoculation. The obtained results will be applied in future investigations on the use of R. Kirilowii extracts in the experimental hypoxia in rats.
Mineral forms in rhizosphere and bulk substrate were examined for selected plant species (Cardaminopsis arenosa, Calamagrostis epigeios, Deschampsia caespitosa, Festuca ovina, Silene vulgaris, Viola tricolor) growing spontaneously in the close vicinity of a tailings pond and in a zone of eolian transmission of waste particles. Samples of substrates and plant roots were taken from sites in the Bolesław orefield near Olkusz. The substrates together with plant roots were studied by scanning electron microscopy coupled with energy dispersive spectroscopy. Apart from quartz and carbonates, the most frequent mineral phases found were crystalline and amorphous Fe oxides, as well as primary Fe, Zn and Pb sulphides (marcasite, pyrite, sphalerite, galena). Hydrated Ca, Mg and Fe sulphates (gypsum, epsomite and melanterite) and other secondary minerals (smithsonite, cerussite, otavite, and Fe, K and Mg aluminosilicates) were found at larger concentrations in the rhizosphere than in bulk soil, suggesting that plant roots can change the mineral composition of the soil.
Grapevine seedlings Vitis vinifera were grown in a greenhouse under optimum conditions (soil moisture ca 70%) and under drought stress (soil moisture ca 35%). In addition, some of the plants subjected to drought underwent subsequent regeneration under optimum conditions. Drought stress caused accumulation of total phenolic compounds in grapevine roots, which may indicate that these compounds play an important role in the adaptation of roots to growth under stress conditions. Phenolic acids found in the roots occurred in the ester-bound form only. p-coumaric acid was present in the highest concentrations (6.2 to 10.5 µg/g fresh matter). The content of ferulic acid was lower, ranging from 2.4 to 4.6 µg/g fresh matter. The lowest concentration in grapevine roots was achieved by caffeic acid (2.4 to 2.9 µg/g fresh matter). The levels of p-coumaric and ferulic acids in roots rose significantly under the drought stress, while the concentration of caffeic acid increased during the post-drought recovery period. This may suggest that some of the phenolic acids protect plants under stress conditions. All the extracts from grapevine roots had antioxidative properties, but the antiradical activity of the extracts obtained from roots subjected to drought stress was inferior to the control. The same extracts were also characterised by depressed reducing power. The results imply that tolerance of grapevine to soil drought may be associated with the value of antioxidative potential in root tissues of these plants.
Extracts of Rhodiola Kirilowii plant roots (several years old plant) were studied by HPLC methods. The aim of these studies was searching for active substances: salidroside, tyrosol, rosavin, cinnamyl alcohol and triandrine. In studied extracts mentioned above substances were not found. On chromatograms only one peak (Rt 8,9 min., maximal absorbance at X =220 nm) of notidentified substance was observed. Further investigations are in progress.
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