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Gypsophila aretioides, a cushion form evergreen plant, is a high potential wild species ideal for the use in rock garden, or as a ground cover in sunny dry areas. This plant has the competence to be developed as a new ornamental species. The purpose of this experiment was to provide an efficient micropropagation protocol for G. aretioides in order to facilitate the availability of this species for further studies of domestication. The influence of various concentrations of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) was investigated for multiplication stage. TDZ at low concentration of 0.05 mg dm−3 resulted in the maximum shoot (9.7) and leaf (42.3) number. The shoots were best rooted on MS medium containing 0.6 mg dm–3 indolebutyric acid (IBA) with 7.8 roots per shoot. Despite achievement of a successful protocol for in vitro multiplication and root induction of Gypsophila, low survival rate was obtained when rooted explants were exposed to ex vitro conditions. This is an important issue, which requires particular consideration and further studies. The possible reasons contributing to the low acclimatization rate of this species are being discussed.
Grafting plays an important role in rhododendron propagation. Successful graft union depends on selection of a suitable stock, grafting season, and method. In this study, three methods (whip, cleft, and side grafting) were compared in two seasons. The stocks were mainly cuttings of R. catawbiense-Hybridum, R. ‘Cunningham's White', but also R. pachytrichum and R. brachycarpum. Scions were taken from several cultivars deriving from R. catawbiense. To shorten the production period, scions were grafted on unrooted stocks, and the results are encouraging. The best stock was R. ‘Cunningham's White', especially when side grafting was applied in spring.
Isatis concstricta Davis (an endemic plant of Turkey) suffers from low propagation rates under natural conditions and is threatened due to fast unplanned urbanisation. The study compared the effects of variants of BA + NAA on shoot regeneration on leaf and petiole explants excised from one week old in vitro regenerated seedlings. MS medium containing 1 mg L–1 BA + 1 mg L–1 NAA induced maximum proliferation on petiole and leaf explants with 13.33 and 12.75 shoots per explant repectively. However, leaf explant induced shoots were sturdy and healthier compared to petiole explant induced shoots. These shoots were rooted on MS medium containing 0.5 mg L–1 IBA and the plants were acclimatized in peat moss and sand (v/v). They grew to flowering under ex vitro conditions. This system of regeneration is advantageous for conventional propagation and the results will help in establishment of a powerful and meaningful micropropagation system for I. constricta.
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Znaczenie minibulw w produkcji ziemniaka w Polsce

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There is inadequate information on the application of rootstocks in the propaga­tion of pomegranate. The aim of this research was to study propagation of pomegran­ate using the stenting method. The experiment was conducted on the basis of a com­pletely randomised design (CRD) with two factors: rootstock (Rj, R2 and R3) and the concentration of indole-3-butyric acid (IBA) (0 and 500 mg/l), in three replications. The rootstocks were 'Gorj-e-Dadashi' (R1), 'Gorj-e-Shahvar' (R2) and 'Gool Safid-e- Ashk-e-Zar' (R3), and the scions were of the cultivarMalas-e-Yazdi. The results indi­cated that rootstock type and IBA treatment influenced bud-take and root formation. The highest bud-take percentage was obtained with R1 in combination with 500 mg/l IBA.
Autumn daffodil (Sternbergia lutea) is a rare and endangered bulbous plant with yellow flowers grown naturally in Iran and unfortunately for different reasons this plant is at the risk of eradication. Natural multiplication rate of S. lutea is low, thus propagation by chipping method is one inexpensive and simple way to overcome this problem. This research was therefore performed to investigate the propagation method in two experiments. In the first experiment, the effects of some plant growth regulators (PGRs) on induction and regeneration of bulblets from bulb chips (propagules) were evaluated. The propagules were prepared by chipping method and treated with indole butyric acid (IBA), gibberellic acid (GA3), benzyl adenine (BA), kinetin (Kin) and cycocel (CCC) at 100 and 200 mg L–1 concentrations for 2 h and then incubated for two months at 20 ±1°C. At the end of incubation, the results showed the highest percentage of concurrent bulblet + root regeneration (CBRR), number and fresh weight of bulblets related to 100 mg L–1 GA3. The highest percentage of only bulblet regeneration (OBR), only root regeneration (ORR) and non-regeneration were obtained at 200 mg L–1 IBA, 100 mg L–1 Kin and control (distillated water: DW) treatments, respectively. In the second experiment, the bulblets that had rooted from each treatment of the first experiment were planted and the produced bulbs were compared. The results indicated that the highest percentage of bulblet sprouting, bulb number and diameter were found at 100 mg L–1 GA3. Therefore, the application of GA3 with mentioned concentration is appropriate for vegetative propagation of this plant by bulb chipping.
Cis pospolity (Taxus baccata L.) na terenie Polski występuje na stanowiskach naturalnych w 33 rezerwatach. Może być odnawiany naturalnie lub sztucznie poprzez rozmnażanie generatywne lub wegetatywne. W Nadleśnictwie Kaliska prowadzone są badania nad restytucją cisa do lasu. Od 1998 roku wysadzono około 4000 sadzonek na 26 powierzchniach w leśnictwach.
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