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The beneficial as well as toxic effects of chromium with regard to its absorption, translocation and accumulation in different parts of plants were reviewed. High concentrations of chromium exhibited severe chlorosis, necrosis and a host of other growth abnormalities and anatomical disorders. The regulation of the mineral metabolism, enzyme activity and other metabolic processes by chromium in plants was discussed.
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Aluminium ions are a serious toxic factor for many organisms living under conditions of the environment. Current views on the mechanisms of aluminium tolerance are pressen ted.
In a pot culture experiment, the effect of calcium chloride (CaCl2) as an ameliorating agent on sodium chloride (NaCl) stress was studied in Dioscorea rotundata plants. Plants were raised in pots and exposed to salinity stress (80 mM NaCl) with or without 5 mM CaCl2. NaCl-stressed plants showed decreased protein and total sugars, and increased free amino acid and proline content. When NaCl treatment was combined with CaCl2, overall plant metabolism was altered, with increased antioxidant enzyme activity, paving the way for partial amelioration of oxidative stress caused by salinity.
Hydrogen peroxide (H2O2) is produced predominantly in plant cells during photosynthesis and photorespiration, and to a lesser extent, in respiration processes. It is the most stable of the so– called reactive oxygen species (ROS), and therefore plays a crucial role as a signalling molecule in various physiological processes. Intra- and intercellular levels of H2O2 increase during environmental stresses. Hydrogen peroxide interacts with thiol-containing proteins and activates different signalling pathways as well as transcription factors, which in turn regulate gene expression and cell-cycle processes. Genetic systems controlling cellular redox homeostasis and H2O2 signalling are discussed. In addition to photosynthetic and respiratory metabolism, the extracellular matrix (ECM) plays an important role in the generation of H2O2, which regulates plant growth, development, acclimatory and defence responses. During various environmental stresses the highest levels of H2O2 are observed in the leaf veins. Most of our knowledge about H2O2 in plants has been obtained from obligate C3 plants. The potential role of H2O2 in the photosynthetic mode of carbon assimilation, such as C4 metabolism and CAM (Crassulacean acid metabolism) is discussed. We speculate that early in the evolution of oxygenic photosynthesis on Earth, H2O2 could have been involved in the evolution of modern photosystem II.
Recent biochemical and genetic studies on hydrogen cyanide (HCN) metabolism and function in plants were reviewed. The potential sources of endogenous cyanide and the pathways of its detoxification are outlined and the possible signaling routes by which cyanide exerts its physiological effects are discussed. Cyanide is produced in plant tissues as the result of hydrolysis of cyanogenic compounds and is also released as a co-product of ethylene biosynthesis. Most cyanide produced in plants is detoxified primarily by the key enzyme P-cyanoalanine synthase. The remaining HCN at non-toxic concentration may play a role of signaling molecule involved in the control of some metabolic processes in plants. So, HCN may play a dual role in plants, depending on its concentration. It may be used in defense against herbivores at high toxic concentration and may have a regulatory function at lower concentration. Special attention is given to the action of HCN during biotic and abiotic stresses, nitrate assimilation and seed germination. Intracellular signaling responses to HCN involve enhancement of reactive oxygen species (ROS) generation and the expression of cyanide-insensitive alternative oxidase (AOX) and ACC synthase (ACS) genes. The biochemical and cellular mechanisms of these responses are, however, not completely understood.
Aluminium toxicity is one of the major factors that limit plant growth and develop­ment in many acid soils. Root cells plasma membrane, particularly of the root apex, seems to be a major target of A1 toxicity. However, strong interaction of A13+ , the main A1 toxic form, with oxygen donor ligands (proteins, nucleic acids, polysaccharides) re­sults in the inhibition of cell division, cell extension, and transport. Although the iden­tification of A1 tolerance genes is under way, the mechanism of their expression re­mains obscure.
Soluble carbohydrates, particularly oligosaccharides, can take part in defense responses preventing and restricting fungal pathogen invasion. Morphological changes were observed and β-glucosidase activity was studied in yellow lupin embryo-axes infected and uninfected with F. oxysporum and cultured for 24 lto 96 hours under conditions of a varying carbohydrate supply. The first disease, symptoms - necrotic pathological changes were observed 48 hours after the .inoculation. A higher intensity of the disease changes as well as growth restriction Cle found in 72- and 96-h embryo axes growing under carbohydrate deficiency. The activity of ậ-glucosidase increased during 24 - 96 hours after inoculation. An especially high increase of the activity was noted in 72- and 96-h embryo axes cultured under carbohydrate deficiency. We suggest that sugars are involved in mechanisms of yellow lupin embryo axis tissues resistance, since they constitute source of precursors for the synthesis of antimicrobial factors of plant defense response.
One of the reasons of yew shoot blight observed in the garden and park :plantations was the infection with Pestalotiopsis funerea fungus. Leaves with sive disease symptoms have been found to have metabolic disturbances luced by this pathogen. Activation of oxidative stress enzymes, L. catalase and oxidase, points to the possibility of inducing defense responses. Among factors modifying the degree of plant affection under natural conditions, the level of nitric nutrition as well as light conditions may play an important role.
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