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The effect of auxin, GA and BAP on potato shoot growth and tuberization was investigated under in vitro condition. The shoot length of potato explants increased with the increasing of concentrations (0.5 - 10 mgdm⁻³) of IAA treatment especially with the addition of GA3 (0.5 mgdm⁻³), but was inhibited by BAP (5 mgdm⁻³). The root number and root fresh weight of potato explants increased with the increasing of IAA levels either in the presence of GA3 (treatment IAA+GA) or not (IAA alone). However, no root was observed in the treatment IAA+BAP, inttead there were brown swolten calli formed around the basal cut surface of the explants. The addition of GA3 remarkably increased the fresh weight and diameter of calli. Micro tubers were formed in the treatments of IAA + BAP and IAA + GA + BAP but not observed in the treatments of IAA alone or IAA + GA. IAA of higher concentrations (2.5 - 10 mgdm⁻³) was helpful to form sessile tubers. With the increastng of IAA levels, the fresh weight and diameter of micro tubers increased progressively. At 10 mg/L IAA, the fresh weight and diameter of microtubers in the treatment of IAA + GA + BAP were 409.6 % and 184.4 % of that in the treatment of IAA + BAP respectively, indicating the interaction effect of GA and IAA in potato microtuberization.
Methyl jasmonate (MeJA) was found to reduce spore germination, hyphal and mycelial growth in Alternaria alternata (Fr.) Keissl. The addition of ethephon or 1-aminocyclopropane-1-carboxylic acid (ACC), ethylene precursor, together with MeJA to the culture medium resulted in a promotion of all developmental stages of the fungus; these compounds partially or completely reversed the inhibition due to MeJA depending on the concentrations applied. MeJA alone had no effect on ethylene production by mycelium, but after 6 days of incubation in the presence of ACC, emanation of this gas increased significantly. Ethylene is involved in reversing the inhibition of A. alternata due to MeJA.
The parasite contamination make difficult laboratory cultivation of green algae and decrease significantly the production of microalgal biomass during industrial cultivation. In the present study the influence of the endogenous abscisic acid content (determinate by gas chromatography) on the host-parasite relationship in different ontogenetic phases of the host Scenedesmus as well as resistance induction after treatment of synchronous algal culture with ABA were studied. Synchronization of algae was carried out by alteration of light and dark periods. The age groups under study were: autospores (at the beginning of the light period), growing cells, mature cells (belonging to in the end of the light period) and mature cells, starting to release autospore at the beginning of dark phases. The higher levels of endogenous ABA during the algal autospore formation as well as exogenous ABA supply of (10-5 M) inhibited the infection process in the pathosystem green microalga Scenedesmus acutus and unicellular fungal parasite Phlyctidium scenedesmi. The treatment with fluridone 10-7 M (an inhibitor ofABA biosynthesis) increased the host susceptibiltty during all ontogenetic phases. The susceptibiltty of S. acutus to the chytridial infection depended on the endogenous ABA level during different ontogenetic stages of the alga.
The effect of cytokinins with different chemical structures, adenine-type: N6-benzylaminopurine (BAP), N6-furfurylaminopurine (FAP) and derivative of urea - 1,3-diphenylurea (DPU) applied in optimal physiological concentrations of 10-6-10-4 M on the mixotrophic plant Wolffia arrhiza during the period of 20 days of culture, was examined. The cytokinin highest influence on the metabolites level in W. arrhiza fresh weight was demonstrated in the range of concentrations 10-6-10-5 M between the 5th and the 10th day of culture. Among used cytokinins, the highest stimulative effect on the content of water-soluble proteins can be attributed to DPU (181%), slightly lower to BAP (167%) and the lowest to FAP (113%) in comparison with the control culture (100%). BAP was found to exert the most stimulative activity on nucleic acids accumulation (DNA+RNA) to the maximum value (127%). FAP and DPU possessed weaker stimulative activity increasing the nucleic acid content to the level of 120% and 118%, properly. The content of monosaccharides was stimulated to the highest level of 174% in FAP treated plants, to 151% in case of BAP and in case of DPU to 144%. Whereas, the highest increase of the photosynthetic pigments content (chlorophyll a and b as well as total carotenoids) to the range of 123-146% in W. arrhiza culture in the presence of DPU, was observed. Under the influence of FAP the accumulation of photosynthetic pigments was stimulated less effectively to the range of 113-140%. When the plants grew with BAP the chlorophylls and carotenoids contents increased to the lowest values of 119-131%. On the other hand, the content of total carotenoids was stimulated only during the first days of culture, then between the 15th and the 20th day it was inhibited by all applied adenine and urea-type cytokinins to the value of 70%. The results indicate that there is no quantitative correlation between the structure of all analysed cytokinins and their influence on the biochemical responses of W. arrhiza.
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