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Identification and quantitative determination of pinoresinol in Taxus x media Rehder needles, cell suspension and shoot cultures

100%

Mistrzak P., Celejewska-Marciniak H., Szypula W. J., Olszowska O., Kiss A. K.

Acta Societatis Botanicorum Poloniae

2015

tom 84

nr 1

The aim of our study was to investigate the presence and quantitative contents of lignans in the tissues of Taxus ×media. The presence of the lignans: pinoresinol, matairesinol and secoisolariciresinol was assessed in needles, shoots cultures and suspension culture. Pinoresinol was the only lignan found in the tissue of T. ×media. The total pinoresinol content in the needles and in the shoots was 1.24 mg/g dry weight (dw) and 0.69 mg/g dw, respectively. Most of the pinoresinol identified was appeared glycosidically bound. In needles, the amount of glycosidically bound pinoresinol (0.81 mg/g dw) was about twice as high as that of free pinoresinol (0.43 mg/g dw). The content of free and glycosidically bound pinoresinol showed the level of 0.18 mg/g dw and 0.51 mg/g dw, respectively in the in vitro shoot cultures. In the cell culture, no pinoresinol was found.

Ograniczanie wyników

1 Acta Societatis Botanicorum Poloniae

1 Celejewska-Marciniak H.

1 Kiss A. K.

1 Mistrzak P.

1 Olszowska O.

1 Szypula W. J.

1 2015

Wyniki wyszukiwania

Identification and quantitative determination of pinoresinol in Taxus x media Rehder needles, cell suspension and shoot cultures