Mango (Mangifera indica L.) is a highly popular tropical fruit. One of the factors causing the low production of mango is fruit drop. Many factors cause fruit drop, one of them being a phytohormonal imbalance. The objective of this research was to compare the content of indole-3-acetic acid (IAA), 1-amino cyclopropane-1- carboxylic acid (ACC), and carbohydrate between persistent and pre-abscission fruits of two cultivars Gadung 21 and Lalijiwo. Pre-abscission fruit had lower IAA and total sugar, but higher ACC and starch than persistent fruit for both cultivars.