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The physiological response of Phalaenopsis × hybridum ‘Innocence’ to biotic stress caused by Pseudococcus longispinus feeding was investigated. The condition of the cytoplasmic membranes expressed by a value of electrolyte outflow (EL) and TBARS and the activity of antioxidative system enzymes: catalase and peroxidase, and the amount of non-enzymatic antioxidant – proline, were determined. The changes in all the analyzed physiological parameters depended on the duration of the pest feeding. The outflow of electrolytes, TBARS content and catalase activity was the highest in the first period of the experiment (after 24-hour of mealybug feeding). Significant increase of peroxidase activity and proline content was noted after 7 days of insects feeding. The values of all analyzed parameters (except EL) demonstrated a decreasing tendency after 14 days of P. longispinus feeding. The observed reaction of P. hybridum ‘Innocence’ testifies to mechanisms triggered with the aim of neutralizing the effects of biotic stress and enabling the normal functioning of the cells in the orchid plants colonized by longtailed mealybug.
A hydroponic experiment has been conducted to determine the physiological and biochemical response of four new rye lines – S120, S76, OT1-3 and 541 – bred at the Department of Plant Genetics and Breeding, Agricultural University in Szczecin. Seeds were sown into soil and placed into a phytotrone chamber with controlled atmosphere and photoperiod. The relative humidity was 80%, the temperature maintained at 20oC and the intensity of photosynthetically active radiation (Phar) was 700 μmol⋅m-2⋅s-1. Seed germination occurred after about 5 days. The seeds were characterised by different germination ability: 80% for S120, 100% for S76, 82% in OT1, and just 50% for 541 line. Rye seedlings, at the 2-3 leaf stage, were placed in hydroponic units and a two-factor experiment was set up, with potassium deficiency being the first factor and rye lines the second one. The control variant consisted of seedlings placed on complete Hoagland’s medium, while potassium starvation was performed on a medium with 50% deficiency of this element. After seven days, fresh matter was weighed, proline content was measured according to the BATES method (1973) and amounts assimilation pigments were measured according to the method of ARNON et al. (1956). The results were processed statistically, performing a two-factor analysis of variance, while the significance of factors was tested using Tukey’s test at α = 0.05. The study aimed at examining selected physiological and biochemical indicators of the resistance response of four rye lines to stress induced by potassium deficiency. Application of 50% potassium deficiency in a medium induced an increase in the content of assimilation pigments in leaf fresh matter of S120 rye line. A correlation was found between proline content and that of assimilation pigments. The largest proline quantity, 27.2 μg⋅g-1 f.m., was found in 541 rye line seedlings, but its content decreased together with fresh matter yield. It was also found that the total chlorophyll content was directly proportional to the content of proline in S120, S76 and OT1-3 rye lines, whereas in the case of other pigments such a relationship existed only for S 120 and S76 rye lines.
The reaction of the rape and corn plants on the nitrogen applied in the form NO₃⁻ or NH₄⁺ was studied under the controlled conditions. The ammonium ions with rape markedly lowered the growth rate, the intensity of photosynthesis and biomass yield, with relation to the plants fertilized with nitrates. With corn, the effect of both N-forms was similar and positive. The biomass of the rape using NH₄⁺, with relation to the plants fertilized with NO₃⁻ showed lower content of total N, and protein N, as well as markedly higher content of amino-acids of primary protein synthesis and their amides and free ammonium ions. Yet, the corn utilizing N-NH₄⁺ showed, as compared with the plants fertilized with NO₃⁻, higher content of all studied organic N fractions, with similar content of free ammonium ions. This paper presents the thesis that the cause of the growth inhibition of rape fertilized with ammonium is low, as compared with corn, assimiliation of NH₄⁺ ions drawn in the roots of this plant.
Triazine resistant and susceptible biotypes of horseweed (Erigeron canadensis L.) were grown under controlled conditions in nutrient solution with NH₄ or NO₃ ions as nitrogen source. Indicators such as: dry matter, CO₂ assimilation, chloroplasts activity, dark respiration and the content of K, Ca, Mg in the above ground parts displayed lower levels when plants were fed with NH₄ than with NO₃ ions. Reduction of these indicators was stronger for the triazine susceptible plants which grew better than their resistant counterparts when fed with N-NO₃ . The triazine resistant plants grown with N- NH₄ contained more protein-N and K than the susceptible ones. Chloroplasts of the resistant plants were also less sensitive to NH₄CL treatment. It is concluded that the resistant plants appear to be more tolerant to NH₄ ions as compared with their susceptible counterparts. For this reason application of ammonium forms of nitrogen fertilization in horticultural practice could favour spreading of triazine resistant horseweed.
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