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The objective of this study was to establish the composition of strawberry preparations rich in ellagitannins obtained using water or acetone extraction (EF and EP preparation, respectively). Then, biological effect of these extracts was assessed in 4-wk nutritional experiment on Wistar rats. The preparations were applied in cholesterol-containing diets that had equal content of ellagitannins (0.03%). To measure animals response, parameters describing the caecal fermentation (ammonia and short-chain fatty acid concentrations, bacterial enzymes activity), blood serum lipoprotein profile, and TBARS content in selected tissues (heart, liver, kidney) were assessed. Apart from polyphenols, including ellagitannins (7.8 and 7.1%, respectively), the EF preparation contained high quantities of soluble dietary fibre and other carbohydrates (33.3 and 38.9%, respectively), whereas the EP preparation was characterised by 58.9% content of ellagitannins, no dietary fibre and a high content of proanthocyanidins (16.9%). In comparison to EF group, the dietary treatment with EP had a stronger effect on caecal environment as manifested by decreased digesta bulk, ß-glucuronidase activity and total short-chain fatty acid concentration (P<0.05 vs. group C without supplementation). Both preparations lowered lipaemia and glycaemia. It could be concluded that more efficient acetone extraction of strawberry pomace increased the content of both ellagitannins and proanthocyanidins in the polyphenolic preparation, which caused a stronger inhibiting effect on caecal fermentation processes and at the same time lowered blood triacyl-glycerols and glucose level. Considering the equal content of ellagitannins in both supplemented diets, it may be speculated that the above effects were due to the presence of proanthocyanidin fraction.
This paper presents results of research concerning bacteria inhabiting the surface microlayer and subsurface waters of the eutrophic lake Jeziorak Maly. Total number, biomass and share of heterotrophic bacteria was higher in the surface microlayer than in subsurface water. The rate of secondary production of bacteria was higher in the subsurface water. There is a considerably higher percentage of neustonic bacteria showing the ability to degrade different compounds than planktonie ones.
The occurrence of bacteria displaying particular physiological properties was studied in polluted (Sopot) and unpolluted (Czołpino) marine sandy beaches (southern Baltic Sea). All eight isolated physiological groups of bacteria were much more numerous in polluted than in unpolluted beach. In polluted beach, bacteria hydrolyzing uric acid (32.5 cells 103 g–1 dry w. of sand) and ammonifying bacteria (32.3 cells 103 g–1 dry w. of sand) were the most numerous, while nitrifying bacteria were the least numerous (0.014 cells 103 g–1 dry w. of sand). In unpolluted beach, bacteria hydrolyzing uric acid (0.66 cells 103 g–1 dry w. of sand) and reducing methylene blue (0.18 cells 103 g–1 dry w. of sand) were the most numerous, while no bacteria producing hydrogen sulphide from organic compounds or bacteria decomposing urea were isolated. In both beaches, considerable differentiation in the distribution of physiological groups of bacteria was found in a horizontal profile i.e. from the waterline to the middle of beach (~60 m). Data concerning horizontal distribution of the physiological groups of bacteria in the sand of the polluted beach show that the majority of those groups was most numerous in the dune. No clear regularity in the distribution of physiological groups of bacteria was found in the horizontal profile of the unpolluted beach. Results of the present study indicate differences in the distribution of the physiological groups of bacteria in the surface (0–5 cm) and subsurface (5–10 cm) sand layers. Generally, in both studied beaches all physiological groups of bacteria were much more numerous in the surface than in the subsurface sand layer. The exception were bacteria reducing sulphates which in the polluted beach were most numerous at the depth of 5–10 cm.
Nonfluorescent pseudomonads have been studied by estimating their numbers in different soils and hortical substrates as well as some physiological properties and antagonistic relationships between them and fluorescent pseudomonads and actinomycetes. Nonfluorescent pseudomonads, depending on the studied soil or substrate, constituted 7-10% of the total number of bacteria from genus Pseudomonas, and the dominant among them were pectinolytic psychrotrophs. Antagonism of nonfluorescent pseudomonads was less frequent and less intense than that of fluorescent pseudomonads as counter-partners. On the other hand, in the system of interrelationships with actinomycetes, the antagonism of nonfluorescent pseudomonads was relatively more frequent, though its intensity was lower.
This paper presents the results of research on the number, the rate of secondary production and physiological properties of neustonic (surface microlayer SM ≈ 250 μm) and planktonic (subsurface water SSW ≈10–15 cm) bacteria of the eutrophic lake (TP 30–99 μg l⁻¹; TN 0.94⁻¹.76 mg l⁻¹; chlorophyll a 26.4–56.9 mg l ⁻¹; water transparency 1.2–1.9 m). It was found that the total number of neustonic bacteria (TNB) varied from 1.28 × 10⁶ to 1.98 × 10⁶ cells ml⁻¹ and was from 1.4 to 2.0 times higher than the number of planktonic bacteria (P <0.001). TNB range for planktonic bacteria oscillated between 0.75 × 10⁶ and 1.45 × 10⁶ cells ml⁻¹. The number of heterotrophic neustonic (SM) bacteria (CFU 22 °C) was also higher by 2.0 to 13.3 times (P <0.001) being between 1.48 and 12.5 × 10³ cells ml⁻¹ while the CFU of bacteria in the SSW oscillated between 0.35 to 0.94 × 10³ cells ml⁻¹. Both the values of TNB and CFU displayed a distinct seasonal variation (P <0.001). However, the rate of secondary production of planktonic bacteria was higher (from 1.1 to 6.0 times) than the rate of production of neustonic bacteria (P <0.05) and displayed seasonal variability (P <0.001). The rate of secondary production in subsurface water ranged from 0.676 to 1.265 μgC l⁻¹ h⁻¹ while in surface microlayer from 0.118 to 0.597 μgC l ⁻¹ h⁻¹. In neuston the bacteria decomposing fat and DNA were more common than in plankton (P <0.05).
Results of studies on a number of colony-forming units of heterotrophic bacteria, their physiological properties and nutritional requirements in water of Lake Jamno estuary are presented. The density of bacteria showed marked seasonal fluctuation with peaking in summer. Most numerous groups among bacteria studied were ammonifying, proteolytic and lipolytic strains. The smallest groups of them consisted of bacteria hydrolysing starch and reducing sulphates. In the water of Lake Jamno bacteria which for optimal growth required a mixture of amino acids (group A), vitamins (group G) and soil extract (group S) predominated. Bacteria having simple nutritional requirements were not numerous among isolated strains.
Research was carried out on the number of heterotrophic bacteria inhabiting the surface of macrophytes submerged in water that are dominant in the littoral zone of Chełmżyńskie Lake: common reed, cattail and hornwort. Bacteria were identified and their ability to decompose certain biopolimers was determined. It was found that the number of bacteria growing on helophytes, i.e. common reed and bulrush, increased from spring to summer, and then decreased in autumn. On the surface of hornwort the number of heterotrophic bacteria increased throughout the whole vegetative season, reaching its maximum in autumn. From among all of the isolated strains, lipo-, cellulo- and proteolytic bacteria were dominant. Strains isolated from the surface of cattail were characterized by the potentially highest physiological activity. The generic composition of the isolated strains changed with the development of the macrophytes (season) and depended on the species of plant.
Studies on the spatial and vertical variability of saprophytic bacteria abundance and their physiological properties were carried out in Sopot (the Gulf of Gdańsk) during the summer of 1998 at four different points along a transect perpendicular to the water line. Colony forming units (CFU) bacterial counts reached 104-106 g dry wt. of sediment -1. Bacteria were most abundant in the dune, and least abundant in the beach sand. Halotolerant bacteria were more numerous than limnotolerant ones. Ammonifying and uric acid decomposing bacteria constituted the most abundant physiological groups; sulphate reducing bacteria were least numerous.
Research was conducted on the number, secondary production and physiological properties of benthic bacteria in the eutrophic Lake Chełmżyńskie. It was found that the number of psychrophilic bacteria (CFU) occurring in the bottom sediments of the studied lake and the rate of bacterial production (BP) were all characterized by a distinct seasonal variability and depended on the location of the study site and the type of bottom sediments (ANOVA, p-value <0.05). The maximum total number of bacteria (TNB), CFU and BP were observed in summer. Muddy sediments were characterized by a greater number of bacteria than sandy ones. A higher number of heterotrophic microorganisms and greater rate of secondary production were found at the sites located in the part of the studied waterbody near the town than at the sites located far from the town. The most numerous benthic bacteria were strains of hydrolyse fat, protein and cellulose. The least numerous were pectinolytic and chitinolytic bacteria.
This paper presents the results of research on the numbers of planktonic bacteria in Lake Chełmżyńskie, their developmental dynamics and their physiological properties. It was found that the total number of planktonic bacteria (TNB) was between 0.01 to 34.90 x 10⁷ cells . ml⁻¹ while the number of heterotrophic bacteria (TVC 22°C) in the water of the research sites in the lake varied between 0.17 to 42.06 x 10² cells . ml⁻¹. The maximum number of bacteria was found in summer, and the minimum in autumn. In the part of the lake near the town (sites I-VI) a distinctly greater number of planktonic bacteria was found in spring and autumn than in the part far from the town (sites VII- IX). Based on Korsh’s bacteriological “Q” index (Q > 1000) the water of Lake Chełmżyńskie is rated as clean. Among the planktonic bacteria of Lake Chełmżyńskie, Gram-negative rods were dominant, and the majority were slowly growing strains. The most numerous among planktonic bacteria were strains that hydrolyse fat, protein, deoxyribonucleic acid and starch. The least numerous were chitinolytic, nitrifying and ureolytic bacteria. Bacteria belonged to genera Flavobacterium, Aeromonas and Alcaligenes dominated in all examined periods.
Research was carried out on the occurrence and physiological properties of heterotrophic bacteria inhabiting water of the River Brda in the Bydgoszcz town section. The highest number of microorganisms was found in water of the studied river in June and August at sites located in the centre of the town, and the lowest in November and March at the site located at the inflow of the river into the town. There were three times more psychrophilic organisms (CFU 20°C) than mesophilic (CFU 37°C) among bacteria. Gram-negative rods constituted about 97% of all the bacteria, of which fast-developing strains constituted from 51 to 61%. The most commonly occurring bacteria in the water of the River Brda were ammonifying bacteria (83.4%), those that reduce nitrates to nitrites (55.5%), those that produce hydrogen sulphide from organic compounds (54.1%), those that hydrolyse protein (45.1%) and fat (44.3%).The least numerous were pectinolytic (0.8%), urealytic (5.8%) and cellulolytic strains (13.6%). The majority of bacteria were capable of carrying out from two to six different physiological processes (83.5% altogether), about 12% of strains carried out only one process, and only 0.1-4.0% of bacteria carried out more than six processes.
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