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Fusarium oxysporum f. sp. psidii and F. solani, causal agents of wilt in guava are highly variable pathogens. This study was conducted on cultural and physiological (temperature and pH) characters. The data revealed that maximum mycelial growth was obtained in potato dextrose agar as semi-solid media i.e. 78.00 mm for F. oxysporum f. sp. psidii; 73. 83 mm for F. solani, while malt extract broth as liquid broth media i.e. 1 385 mg mycelia for F. oxysporum f. sp. psidii; 1491 mg for F. solani. Maximum sporulation was recorded in oatmeal agar and mycological broth. The optimum temperature and pH for growth of both Fusarium spp. isolates was 28°C and 5.5. The isolates differed in their colony growth; mycelial mass, macro-conidia, and micro-conidia produced. These variations were characters of each of the isolates with respect to cultural and physiological characters.
This research was conducted to evaluate the effects of water salinity and nano-silica (NS) and humic acid (HA) on Bermuda grass. The study was carried out under greenhouse and exterior space conditions in a completely randomized design with factorial arrangements. Treatments included 4 water salinity levels (0, 5, 7 and 9 dS/m) and 4 level of NS (0, 1, 2 and 3 mm/l) as well as 4 level of HA (0, 50, 100 and 150 mg/l). Results indicated a decrease in chlorophyll content, and increase in proline, malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD) and electrolyte leakage (EL) with increasing levels of salinity. The chlorophyll content in greenhouse and exterior space also increased with increasing levels of NS and HA, while proline in two environments, with increasing levels of NS and HA decreased. EL with increasing levels of NS fluctuated, but with increasing levels of HA, it decreased in two environments. With increasing levels of NS, CAT in both environments was eventually decreased after the oscillation, but there was no particular trend in HA levels. With increasing levels of NS and HA, MDA in the greenhouse decreased and in the exterior space – it increased. Finally, by increasing levels of NS and HA, SOD did not show any change in the greenhouse condition, but in the exterior space, the SOD was decreased.
Bakanae disease on rice has been recorded almost in all countries where paddy is grown commercially, especially in Asian countries, including Malaysia and Indonesia. Bakanae disease was widespread in Peninsular Malaysia and three provinces of Indonesia with the range of disease severity from scale 1 to 5 and disease incidence from 0.5 to 12.5% during 2004-2005 main growing seasons. A total of five Fusarium species belonging to section Liseola and their allied i.e. Fusarium fujikuroi, F. proliferatum, F. sacchari, F. subglutinans and F. verticillioides were isolated and identified on the basis of their morphological characteristics. Literature data showed that the bakanae disease of rice all over the world is caused by F. fujikuroi and probably some other Fusarium species from section Liseola or allied. However, from pathogenicity tests that have been carried out by using variety MR 211 of rice it was evident that F. fujikuroi was highly virulent and the only species involved in causing bakanae disease. Therefore, this species was the only one detected to be able to produce gibberellic acid - (GA3) with Rf value 0.40 and 0.62, developed in solvent systems isopropanol:ammonia:water (10: 1: 1), v/v/ v and chloroform:ethyl acetate:formic acid (5: 4: 1), v/v/v, respectively. This knowledge would be invaluable in developing our understanding on the interaction between F. fujikuroi and the host plants.
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