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1

Hemolytic and physiological activities of mixtures of organophosphorous herbicides

100%
Kleszczynska H., Bonarska D., Bielecki K., Sarapuk J.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
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nr Suppl.
2

Motifs with potential physiological activity in food proteins - BIOPEP database

75%
Iwaniak A., Dziuba B.
Acta Scientiarum Polonorum. Technologia Alimentaria
|
2009
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tom 08
|
nr 3
59-85
Proteins are the multifunctional food components affecting the living organ-isms. One of the proteins function is the impact on the body due to the presence of motifs that show specific physiological and biological activities. Due to the worldwide growth of demand for the food containing bioactive components, increasing attention has been paid recently to the use of bioactive peptides as physiologically active food ingredients. They are important elements of the prevention and treatment of various lifestyle diseases. In addition to its primary function and according to current knowledge, each protein may be a reserve source of peptides controlling the life processes of organisms. For this reason, in this work, application of a new, additional criterion for evaluating proteins as a potential source of biologically active peptides, contributes to a more comprehensive and objective definition of their biological value. A complementary part of such research is the strategy for evaluation of the food proteins as precursors of biologically active peptides which involves the database of proteins and bioactive peptides - BIOPEP (available on-line at: http://www.uwm.edu.pl/biochemia). The database contains information on 2123 peptides representing 48 types of bioactivities, their EC50 values and source of origin. Proteins (706 sequences) are considered as bioactive peptide precursors based on newly introduced criteria: the profile of potential biological activity, the frequency of bioactive fragments occurrence and potential biological protein activity. This original and unprecedented so far approach, started to be successfully and more widely applied by other authors. BIOPEP can be interfaced with global databases such as e.g. TrEMBL, SWISS-PROT, EROP and PepBank. Recently the BIOPEP database was enlarged with the data about allergenic proteins, including information about structure of their epitopes and molecular markers
3

Bacteriological investigation of the surface microlayer of the Gulf of Gdansk

75%
Mudryk Z., Korzeniewski K., Falkowska L.
Oceanologia
|
1991
|
tom 30
4

The hemolytic and physiological activities of mixtures of some phenoxy and organophosphorous herbicides

75%
Kleszczynska H., Bonarska D., Bielecki K., Sarapuk J.
Cellular and Molecular Biology Letters
|
2003
|
tom 08
|
nr 1
Experiments were performed investigating the potential to improve the biological activity of some phenoxy and organophosphorous compounds by using them in binary mixtures. The compounds were: 2,4-dichlorophenoxyacetic acid (1) and its sodium salt (2), dibutyl 1-butylamino-l-cyclohexanephosphonate (3) and diethyl 9-butylamino-9-fluorenephosphonate (4), all widely used as herbicides. There were two test methods: the inhibition of cucumber (Cucumis sativus) growth induced by one single herbicide or by equimolar binary mixtures of herbicides; and, in parallel, the hemolytic efficiency of separate compounds or their mixtures. The hemolytic properties of the compounds were studied as hemolysis is generally a good measure of their toxicity, especially in the case of lipophilic compounds. Pig erythrocytes were used as good models for the determination of toxicity and the kinetics of red blood cell hemolysis. In the plant-based experiments, binary mixtures were found to display additive type toxicity. The compounds’ hemolytic activities were of additive or antagonistic types. In some combinations, the addition of a second component did not change the hemolytic efficiency of the first component, and vice versa.
5

Distribution and physiological activity of heterotrophic benthic bacteria in lakes with different trophic conditions located in the Bory Tucholskie National Park (Poland)

51%
Kubera L., Donderski W.
Polish Journal of Natural Sciences
|
2017
|
tom 32
|
nr 3
Research on ecophysiology of benthic bacteria indicates that microbiological processes which occur in bottom sediments have a major influence on the development of water bodies. Organic matter accumulated on the bottom makes lakes shallower and accelerates their aging. Microbiological processes are particularly important in small lakes, which are a common element of the environment. This article presents the results of the investigation of the distribution of heterotrophic bacteria in the bottom sediments of four lakes with different trophic conditions in the Bory Tucholskie National Park and the relationship between the number of benthic bacteria and their physiological activity. The total number of benthic bacteria and the ability of isolated strains to carry out selected physiological processes were assessed by cultivating bacteria on appropriate substrates. We also determined the coefficient of physiological activity, which was then used for the correlation analysis and verification of the research hypothesis. The highest number of benthic bacteria was found in the eutrophic and dystrophic lakes. The lowest number was recorded in lake sediments of the mesotrophic lake, although at this site the studied strains were the most active. As a result, the correlation analysis confirmed the hypothesis that there is no relationship between the metabolic potential of benthic bacteria and their number in a sample.
6

Modulation of physiological and pathological activities of lysozyme by biological membranes

51%
Trusova V.
Cellular and Molecular Biology Letters
|
2012
|
tom 17
|
nr 3
The molecular details of interactions between lipid membranes and lysozyme (Lz), a small polycationic protein with a wide range of biological activities, have long been the focus of numerous studies. The biological consequences of this process are considered to embrace at least two aspects: i) correlation between antimicrobial and membranotropic properties of this protein, and ii) lipid-mediated Lz amyloidogenesis. The mechanisms underlying the lipid-assisted protein fibrillogenesis and membrane disruption exerted by Lz in bacterial cells are believed to be similar. The present investigation was undertaken to gain further insight into Lz-lipid interactions and explore the routes by which Lz exerts its antimicrobial and amyloidogenic actions. Binding and Förster resonance energy transfer studies revealed that upon increasing the content of anionic lipids in lipid vesicles, Lz forms aggregates in a membrane environment. Total internal reflection fluorescence microscopy and pyrene excimerization reaction were employed to study the effect of Lz on the structural and dynamic properties of lipid bilayers. It was found that Lz induces lipid demixing and reduction of bilayer free volume, the magnitude of this effect being much more pronounced for oligomeric protein.
7
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Fluorescencyjna hybrydyzacja in situ w identyfikacji i ocenie aktywności fizjologicznej Lactobacillus spp. w soku z jagód goji

51%
Kocot A. M., Mruk M. S., Olszewska M. A.
Żywność Nauka Technologia Jakość
|
2017
|
tom 24
|
nr 2
W związku z popularyzacją trendu „Super Food” konsumenci oczekują obecności na rynku produktów o szerokim spektrum oddziaływania na organizm człowieka. Sok z jagód goji charakteryzuje się wieloma dobroczynnymi właściwościami, a wzbogacenie go w potencjalnie probiotyczne kultury Lactobacillus spp. może dodatkowo zwiększyć jego wartość biologiczną pod warunkiem zachowania przez szczepy żywotności. Celem pracy była identyfikacja i ocena aktywności fizjologicznej komórek Lactobacillus spp. w soku z jagód goji z zastosowaniem metody fluorescencyjnej hybrydyzacji in situ (FISH). W celu identyfikacji Lactobacillus spp. przeprowadzono hybrydyzację z sondami oligonukleotydowymi rRNA, w tym z sondą dla domeny Eubacteria (Eub338), nonsensowną (Non338), a także dla grupy bakterii fermentacji mlekowej (Lab158) i 11 sond specyficznych dla różnych gatunków z rodzaju Lactobacillus. Analizę aktywności fizjologicznej komórek Lactobacillus spp. w soku z jagód goji przeprowadzono sondą Eub338 bezpośrednio po zaszczepieniu oraz 3., 8., 15., 22. i 29. dnia przechowywania w temp. 20 ºC (± 2 ºC) oraz 6 ºC i porównano z wynikami metody płytkowej. Metoda FISH pozwoliła na określenie przynależności gatunkowej 8 z 9 badanych szczepów, które zaklasyfikowano do L. plantarum oraz L. brevis. Wykazano ponadto, że w wyniku przechowywania soku z jagód goji w temp. 20 ºC (± 2 ºC) aktywność fizjologiczna komórek podlegała znacznym zmianom, czyli zmniejszyła się w ostatnim dniu przechowywania i po porównaniu z wynikami metody płytkowej można wskazać na stan niehodowalności komórek. Natomiast po przechowywaniu w temp. 6 ºC aktywność fizjologiczna była stabilna, gdyż liczba komórek utrzymywała się na wysokim poziomie do ostatniego dnia przechowywania. Badania przeprowadzone w tym zakresie wskazują na przydatność metody FISH w detekcji i poznawaniu fizjologii pałeczek Lactobacillus spp.
8

The necessity of adequate nutrition with diets containing omega-3 and omega-6 fatty acids for proper brain development, function and delayed aging: Review

51%
Kochman K., Czauderna M.
Journal of Animal and Feed Sciences
|
2010
|
tom 19
|
nr 4
9

Rola tauryny w zywieniu

51%
Oledzka R.
Bromatologia i Chemia Toksykologiczna
|
1995
|
tom 28
|
nr 3
287-291
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Species differentiation of yeasts and yeast-like organisms in the water and bottom sediments of the Szczecin Lagoon

51%
Boguslawska-Was E., Dabrowski W.
Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline
|
1999
|
tom 03
11
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Physiological activity of common osier (Salix viminalis L.) under copper-induced stress conditions

51%
Malinowska K., Wrobel J.
Acta Agrophysica
|
2015
|
tom 22
|
nr 2
The study examined the influence of copper salt at concentrations ranging from 50 to 250 mg dm-3 on the physiological reaction of the Bjor and Jorr varieties of common osier. The content of assimilation pigments, the intensity of CO2 assimilation, transpiration, stomatal conductance for CO2 and water vapour, coefficients of the relative water content and water saturation deficit were determined. The intensity of CO2 assimilation, transpiration and the assimilation pigment content in the tested common osier cultivars varied with relation to the copper salt dose in the medium. A decrease in the intensity of the tested physiological parameters was found together with an increase in the copper salt dose in the medium. CO2 assimilation and transpiration of the Bjor and Jorr osier was significantly limited by the stomata. The addition of copper salts to the medium resulted in an increase in the water saturation deficit in leaves of the tested common osier varieties.
12
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Physiological activity of ectomycorrhizas in a moderately polluted forest (Ratanica catchment, Southern Poland)

51%
Kieliszewska-Rokicka B., Kurczynska E. U., Leski T.
Dendrobiology
|
2000
|
tom 45
Ectomycorrhizas of Scots pine (Pinus sylvestris L.) and beech (Fagus sylvatica L.) were sampled in a mature forest ecosystem exposed for more than 40 years to moderate levels of gaseous and dust pollutants. Soil of the forest site was characterised by low pH and accumulation of heavy metals (Pb, Mn, Zn, Cu, Cd, Fe). Mycorrhizal vitality and enzyme activity of the root-surface and soil acid phosphatase (AcPase) were studied at 17 measurement points (0–5 cm soil depth) in relation to the content of inorganic phosphate (Pi) and aluminium ions (Al3+) in the soil. Anatomy of Scots pine and beech mycorrhizas taken from different measurement points was observed. The concentration of essential nutrients (C, N, P, Ca, Mg) and the ratios Ca/Al, Mg/Al and N/P were analysed in fine roots. High concentrations of Al3+ in the soil (40–118 meq kg-1) and low levels of Pi (12–44 mg P2O5 kg-1) were accompanied by high activity of the root-surface AcPase of pine and beech mycorrhizas (25–67 and 33–86 μmol pNP g-1 fresh weight h-1, respectively) and soil AcPase (6.8–22.4 μmol pNP g-1 dry weight h-1). The results indicate that fine tree roots are undoubtedly under stress as evidenced by a disturbance in P uptake and accumulation. However, the high vitality of mycorrhizas and the high Ca/Al ratio in fine roots suggest that the defence mechanisms of mycorrhizas and the rhizosphere are still able to ameliorate the influence of anthropogenic pollution.
13

Odporność: wrodzona i nabyta odpowiedź immunologiczna u krów w okresie przejściowym

38%
McKay S.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2015
|
tom 10
|
nr 3
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