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1

Comparison of the photosensitivity of biofilms of different genera of cariogenic bacteria in tooth slices

100%
Darmani H., Tawalbeh K. H., Al-Hiyasat A. S., Al-Akhras M.-A.
Polish Journal of Microbiology
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2018
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tom 67
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nr 4
This study compared the outcome of photosensitization on the viability of four different cariogens in planktonic form as well as biofilms in human dentine. Photodynamic therapy was carried out with a gallium aluminium arsenide laser (670 nm wavelength) using Toluidine blue O (TBO) as the photosensitizer. Cariogenic bacteria (Streptococcus mutans, Lactobacillus casei, Streptococcus salivarius and Actinomyces viscosus) were exposed to TBO and then to the laser for 1 minute in planktonic suspension. Then, tooth slices previously incubated for 24 hours with broth cultures of broth culture of the four cariogenic organisms were exposed to antimicrobial photosensitization. The control samples consisted of planktonic and sessile cells that were exposed to TBO alone, laser alone and the bacterial cells that were not treated with TBO or laser. The results showed significant reductions in the viability of S. mutans, L.casei and A. viscosus in both planktonic form (to 13%, 30%, and 55%, respectively) and sessile form hosted in dentinal tubules (to 19%, 13% and 52%, respectively), relative to the controls. S.salivarius was the least affected in planktonic (94% viability) and sessile form (86% viability). In conclusion, sensitivity to photosensitization is species-dependent and sessile biofilm cells are affected to the same extent as their planktonic counterparts.
2
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Arabidopsis lipocalins AtCHL and AtTIL have overlapping functions essential for lipid protection and seed longevity

100%
Simona B.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2013
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tom 94
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nr 2
3

The distribution of peripheral blood dendritic cells assayed by a new panel of anti-BDCA monoclonal antibodies in healthy representatives of the Polish population

100%
Narbutt J., Lesiak A., Zak-Prelich M., Wozniacka A., Sysa-Jedrzejowska A., Tybura M., Robak T., Smolewski P.
Cellular and Molecular Biology Letters
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2004
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tom 09
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nr 3
A growing number of studies are being performed on the role of dendritic cells (DCs) in the etiopathogenesis of various conditions. Therefore, it is extremely important to establish the best comparable methods for the determination of the absolute count of blood dendritic cells (BDCs) or their subsets, and the reference normal values for comparisons. The aim of our study was to assess a normal profile of BDCs in the non-cultured human blood of healthy Polish volunteers. BDCs were detected among peripheral blood mononuclear cells (PBMC) from 99 healthy people, aged 18- 56. Based on the panel of novel anti-BDCA1, BDCA2 and BDCA3 monoclonal antibodies (MoAbs), three main subpopulations of BDCs were distinguished: two myeloid types of BDCs, MDC1(BDCA-1+/ CD11c+ /HLA-DR+) or MDC2 (BDCA-3+/CD32-/CD64-/HLA-DR+), and a plasmacytoid subtype, PDC (BDCA-2+/CD123+/HLA-DR+). The number and percentage of BDCs were correlated with the age, gender, photosensitivity (phototype, minimal erythemal dose - MED) and morphological parameters of the healthy volunteers. BDCs represented 0.83% of the PBMC and the median total BDC number was 44.0 cell/ml. The total BDC number correlated with the WBC count (r=0.40; p<0.001) as well as with the lymphocyte and monocyte counts (r=0.20; p=0.045 and r=0.26; p=0.009, respectively). The median percentage of the MDC1 count (0.20%) was twice as high as the MDC2 count (0.10%). The median PDC count was 28.2 cell/ml, and these cells represented 0.50% of the PBMC. There was a positive correlation between PDC and skin photosensitivity (r=0.28; p=0.005). An inverse correlation between the PDC count and the age of the examined volunteers was also found (r=-0.22; p=0.029). Our study provides the first referential data on normal rates and counts of BDCs and their subpopulations, assessed by the new panel of anti-BDCA MoAbs, in healthy Polish subjects. The method used in the study allowed the determination of BDCs and their subset numbers in a relatively small blood volume.
4

Heat- and photosensitivities of chlorophyll protein complexes in thylakoid membranes isolated from cucumber cotyledons

80%
Siwiak A., Waloszek A., Wieckowski S.
Acta Physiologiae Plantarum
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1994
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tom 16
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nr 2
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