Wyniki wyszukiwania

1

The influence of alkylresorcinols on phospholipase A2 activity

100%

Czajkowska D., Mika J., Stasiuk M., Kozubek A.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr Suppl.

2

The role of phospholipases A2 in the pathophysiology of acute pancreatitis. A review

86%

Hietaranta A. J., Kemppainen E. A., Gronroos J. M., Nevalainen T. J.

Journal of Physiology and Pharmacology. Supplement

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1998

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tom 49

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nr 2

3

Lysophosphatidylcholine triggers cell differentiation in the protozoan parasite Herpetomonas samuelpessoai through the CK2 pathway

72%

Dutra F. L., Vieira D. P., Coelho F. S., Adade C. M., Atella G. C., Silva Neto M. A. C., Lopes A. H.

Acta Parasitologica

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2020

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tom 65

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nr 1

4

Axenic cultivation and comparative phospholipase A2 activity of Giardia duodenalis in a serum-free medium

72%

Mata-Cardenas B. D., Hernandez-Garcia M. E., Gonzalez-Salazar F., Garza-Gonzalez J. N., Palacios-Corona R., Cortes-Gutierrez E. I., Vargas-Villarrewal J.

Acta Parasitologica

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2012

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tom 57

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nr 3

Mammalian serum is essential for the growth of Giardia duodenalis cultivated under axenic conditions. Unfortunately, some factors present in bovine serum used as supplement in the culture medium may inhibit protozoal growth and activity. TYI-33-PACSR is a TYI medium supplemented with a serum replacement (PACSR) made up of Earle’s amino acid solution, Diamond’s vitamin-tween 80 mixtures and LCR (a lipid-cholesterol — rich mixture). PACSR was previously used in the culture media for axenic cultivation of Entamoeba histolytica and Trichomonas vaginalis. The main objective of this work was to demonstrate that TYI-33-PACSR is useful for axenic cultivation of G. duodenalis. Additionally, the activity of phospholipase A2 (PLA A2) in the sub-cellular vesicular fraction (P30) of G. duodenalis grown in TYI-S-33 and TYI-33-PACSR was compared. All strains of Giardia grown in TYI-33-PACSR reached relative cellular densities of 91 to 95% compared to controls growing in serum-supplemented TYI-S-33 medium. Additionally, PLA A2 activity was similar in the P30 sub-cellular fraction obtained from trophozoites growing in TYI-S-33 and TYI-33-PACSR. Thus, TYI-33-PACSR could be useful in analyzing the biological properties of G. duodenalis in the absence of serum.

5

Trichomonas vaginalis acidic phospholipase A2: isolation and partial amino acid sequence

72%

Escobedo-Guajardo B. L., Gonzalez-Salazar F., Palacios-Corona R., Cruz V. M. T., Morales-Vallarta M., Mata-Cardenas B. D., Garza-Gonzalez J. N., Rivera-Silva G., Vargas-Villarreal J.

Acta Parasitologica

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2013

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tom 58

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nr 4

Sexually transmitted diseases are a major cause of acute disease worldwide, and trichomoniasis is the most common and curable disease, generating more than 170 million cases annually worldwide. Trichomonas vaginalis is the causal agent of trichomoniasis and has the ability to destroy in vitro cell monolayers of the vaginal mucosa, where the phospholipases A2 (PLA2) have been reported as potential virulence factors. These enzymes have been partially characterized from the subcellular fraction S30 of pathogenic T. vaginalis strains. The main objective of this study was to purify a phospholipase A2 from T. vaginalis, make a partial characterization, obtain a partial amino acid sequence, and determine its enzymatic participation as hemolytic factor causing lysis of erythrocytes. Trichomonas S30, RF30 and UFF30 sub-fractions from GT-15 strain have the capacity to hydrolyze [2-14C-PA]-PC at pH 6.0. Proteins from the UFF30 sub-fraction were separated by affinity chromatography into two eluted fractions with detectable PLA A2 activity. The EDTA-eluted fraction was analyzed by HPLC using on-line HPLC-tandem mass spectrometry and two protein peaks were observed at 8.2 and 13 kDa. Peptide sequences were identified from the proteins present in the eluted EDTA UFF30 fraction; bioinformatic analysis using Protein Link Global Server charged with T. vaginalis protein database suggests that eluted peptides correspond a putative ubiquitin protein in the 8.2 kDa fraction and a phospholipase preserved in the 13 kDa fraction. The EDTA-eluted fraction hydrolyzed [2-14C-PA]-PC lyses erythrocytes from Sprague-Dawley in a time and dose-dependent manner. The acidic hemolytic activity decreased by 84% with the addition of 100 μM of Rosenthal’s inhibitor.

6

The effect of reactive oxygen species on the synthesis of prostanoids from arachidonic acid

58%

Korbecki J., Baranowska-Bosiacka I., Gutowska I., Chlubek D.

Journal of Physiology and Pharmacology

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2013

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tom 64

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nr 4

7

Differences between group X and group V secretory phospholipase A2 in lipolytic modification of lipoproteins

58%

Kamitani S., Yamada K., Yamamoto S., Ishimoto Y., Ono T., Saiga A., Hanasaki K.

Cellular and Molecular Biology Letters

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2012

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tom 17

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nr 3

Secretory phospholipases A2 (sPLA2s) are a diverse family of low molecular mass enzymes (13–18 kDa) that hydrolyze the sn-2 fatty acid ester bond of glycerophospholipids to produce free fatty acids and lysophospholipids. We have previously shown that group X sPLA2 (sPLA2-X) had a strong hydrolyzing activity toward phosphatidylcholine in low-density lipoprotein (LDL) linked to the formation of lipid droplets in the cytoplasm of macrophages. Here, we show that group V sPLA2 (sPLA2-V) can also cause the lipolysis of LDL, but its action differs remarkably from that of sPLA2-X in several respects. Although sPLA2-V released almost the same amount of fatty acids from LDL, it released more linoleic acid and less arachidonic acid than sPLA2-X. In addition, the requirement of Ca2+ for the lipolysis of LDL was about 10-fold higher for sPLA2-V than sPLA2-X. In fact, the release of fatty acids from human serum was hardly detectable upon incubation with sPLA2-V in the presence of sodium citrate, which contrasted with the potent response to sPLA2-X. Moreover, sPLA2-X, but not sPLA2-V, was found to specifically interact with LDL among the serum proteins, as assessed by gel-filtration chromatography as well as sandwich enzyme-immunosorbent assay using anti-sPLA2-X and anti-apoB antibodies. Surface plasmon resonance studies have revealed that sPLA2-X can bind to LDL with high-affinity (Kd = 3.1 nM) in the presence of Ca2+. Selective interaction of sPLA2-X with LDL might be involved in the efficient hydrolysis of cell surface or intracellular phospholipids during foam cell formation.

8

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Neutrophils-induced increase of adenosine triphosphate depletion in rat neonatal cardiac myocytes with impaired energy metabolism

58%

Gajewski M., Laskowska-Bozek H., Maslinski S., Ryzewski J.

Journal of Physiology and Pharmacology

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1991

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tom 42

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nr 4

Isolated, cultured rat neonatal cardiac myocytes were placed in medium suppled menfed with mitochondrial respiratory inhibitor potassium cyanide which caused a rapid adenosine triphosphate (ATP) depletion. These myocytes with the impaired energy metabolism (“hypoxia-like state”) were exposed to unstimulated human neutrophils. Effect of human neu rophils on the myocy es m the “hypoxia-like state” was quantified as a total change in the amount of ATP in cardiac cells. After 5 hours of incubation of neu rophils with the myocytes in the “hypoxia-like state” an additional decrease (of 50 per cent) in ATP content was observed. Since catalase (which destroys hydrogen peroxide) prevented the further decline in ATP level in the myocy es with impaired energy metabolism, it seem that hydrogen peroxide and possibly their products are responsible for this effect. These results suggest that unstimulated human neu rophils af er activation by the contact with injured cardiac cells caused further decrease of ATP level in target cells.

9

Light activation of phospholipase A2 in the photoreceptor of the crayfish [Procambarus clarkii]

58%

Kashiwagi T., Meyer-Rochow V. B., Nishimura K., Eguchi E.

Acta Neurobiologiae Experimentalis

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2000

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tom 60

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nr 1

Retinal lipids of crayfish, kept at 4°C under continuous darkness for 3 weeks, consisted mainly of phosphatidylcholine (PC) and phosphatidylethanolamine (PE); sphingomyelin (SM), phosphatidylinositol (PI) and phosphatidylserine (PS) were minor contributors. PI, involved in the phototransduction cascade, never reached greater concentrations than 7% of the total. High concentrations of polyunsaturated fatty acids (PUFA) such as 20:4n-6, 20:5n-3 and 22:6n-3 (DHA, docosahexaenoic acid) were present in PC, PE and PS, but scarce in SM and PL In retinae of crayfish kept at 4°C in darkness for 3 weeks and then exposed to white light (6 h; ca. 4,500 lx), SM and PS remained seemingly unaffected. PC, however, significantly decreased within 10 min to 65% of the initial value and 50% at 180 min. To study the reduction of PC, lipids of retinae suspended in physiological solution with/without phospholipase C (PLC) and phospholipase A2 (PLA2) inhibitors such as DMDA (=DEDA), manoalide, ET-I8-OCH3, and U-73122 were measured. Only free fatty acids (FFA) of retinae with inhibitors of PLA2 like DMDA and manoalide decreased. Retinae irradiated by white light for 3 h displayed a significant reduction of PC, compared with those that had remained in continuous darkness. However, the PC of retinae with PLA2-inhibitors was not decreased by light. Our results provide evidence that not only photoreceptor cell PLC, but also PLA2 is activated by light.

10

Jak atakuje Helicobacter pylori?

51%

Lekowska-Kochaniak A.

Żywność, Żywienie a Zdrowie

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1995

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tom 04

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nr 1-4

44-48

Ograniczanie wyników

The influence of alkylresorcinols on phospholipase A2 activity

The role of phospholipases A2 in the pathophysiology of acute pancreatitis. A review

Lysophosphatidylcholine triggers cell differentiation in the protozoan parasite Herpetomonas samuelpessoai through the CK2 pathway

Axenic cultivation and comparative phospholipase A2 activity of Giardia duodenalis in a serum-free medium

Trichomonas vaginalis acidic phospholipase A2: isolation and partial amino acid sequence

The effect of reactive oxygen species on the synthesis of prostanoids from arachidonic acid

Differences between group X and group V secretory phospholipase A2 in lipolytic modification of lipoproteins

Neutrophils-induced increase of adenosine triphosphate depletion in rat neonatal cardiac myocytes with impaired energy metabolism

Light activation of phospholipase A2 in the photoreceptor of the crayfish [Procambarus clarkii]

Jak atakuje Helicobacter pylori?