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Herba Polonica
|
2009
|
tom 55
|
nr 3
140-146
The chemical diversity within wild strawberry (Fragaria vesca L.) species was studied depending on its origin (natural vs cultivated). The herb was collected at the full flowering stage. Following items were determined in collected material: flavonoids, phenolic acids, tannins, anthocyanins, as well as anti-oxidation activity (%) was evaluated by means of ability to neutralize the DPPH radicals. Contents of studied biologically active substances depended on the plant origin. Extracts made from examined raw materials showed no differences in the ability to reduce DPPH radicals to diphenylopicrylohydrazine.
Herba Polonica
|
2009
|
tom 55
|
nr 3
153-162
The qualitative and quantitative composition of essential oils obtained from whole leaves and inflorescences of two cultivars wild strawberry (΄Rugia` and ΄Baron von Solemacher` cv.) by means of steam and xylene distillation method, was compared. Material harvested at full flowering stage in 2008 and 2009 was subjected to study. Depending on a cultivar, air-dry inflorescences from wild strawberry contain from 0.21% (΄Baron von Solemacher` cv.) to 0.30% (΄Rugia` cv.), whereas leaves contains from 0.46% (΄Baron von Solemacher` cv.) to 0.62% (΄Rugia` cv.) of essential oils. GC/MS analysis of essential oils achieved from studied materials revealed presence of 70 (including 59 identified) compounds in leaves of ΄Rugia` cv. and 58 (including 50 identified) compounds in leaves of ΄Baron von Solemacher` cv. Essential oils from inflorescences of ΄Rugia` cv. contained 52 (including 47 identified), while ΄Baron von Solemacher` cv. contained 54 (including 46 identified) compounds. The chromatographic analyses by GC-MS revealed that myrthenol, nonal, linalool and phthalide dibuthyl dominated in essential oils obtained from leaves, while myrthenol, citronelol, linalool and geraniol – from those of inflorescences. There were qualitative differences between oil components at both studied materials and differentiation between both cultivars, as well.
This review is based on both classical and newest original research publications found in prominent journals on plant genetic resources. It exemplifies, illustrates and summarizes statistical methodology for analyzing multivariate data from trials and surveys applied to evaluate phenotypic diversity in germplasm collections. Specific methodological knowledge necessary to select appropriate techniques, carry out data analysis and make proper interpretations of analytical results is discussed. Those uni- and multivariate statistical methods proved effective for evaluating phenotypic diversity in plant genetic resources for quantitative traits are shortly characterized and discussed with respect to their use with the SAS package. Univariate methods are based on random ANOVA model for data originating from a trial carried out in a randomized complete block design and also in a multi-environment trials carried out in the same block design. Pattern analysis is mainly recommended, among multivariate techniques, as an effective procedure to evaluate quantitative phenotypic diversity in the germplasm collections. It is a combined approach including cluster analysis as a classification procedure and principal component analysis or canonical variant analysis as the ordination procedures to show graphically similarities and dissimilarities among accessions and their homogenous groups.
Celem badań była wielocechowa charakterystyka zmienności dziesięciu cech osiemnastu geno-typów lnu uprawnego (Linum usitatissimum L.). Zastosowano metodę analizy zmiennych kanonicznych opartą na modelu wielowymiarowej analizy wariancji dla obserwowanych cech. Materiałem do badań było: dwanaście rodów hodowlanych i sześć odmian lnu uprawnego. Doświadczenia założono w układzie całkowicie losowym w trzech powtórzeniach, w dwóch sezonach wegetacji (2008, 2009) w Zakładzie Doświadczalnym IWNiRZ w Wojciechowie. Rody i odmiany oceniono pod względem dziesięciu cech ilościowych. Przeprowadzona wielowymiarowa analiza wariancji wykazała istotne zróżnicowanie genotypów, jak również zróżnicowanie ekspresji genotypów w latach. Zastosowana metoda zmiennych kanonicznych wykazała różne zachowanie genotypów w poszczególnych latach. Stwierdzono istotną statystycznie korelację odległości Mahalanobisa otrzymanych w obu latach: r = 0,5942, P < 0,001. Genotypy charakteryzujące się największym zróżnicowaniem genetycznym mogą posłużyć do tworzenia odrębnych pul genetycznych lnu uprawnego.
Cystic fibrosis (CF) is one of the most common autosomal recessive diseases among Caucasians caused by a mutation in the CFTR gene. However, the clinical outcome of CF pulmonary disease varies remarkably even in patients with the same CFTR genotype. This has led to a search for genetic modifiers located outside the CFTR gene. The aim of this study was to evaluate the effect of functional variants in prostaglandin-endoperoxide synthase genes (COX1 and COX2) on the severity of lung disease in CF patients. To the best of our knowledge, it is the first time when analysis of COX1 and COX2 as potential CF modifiers is provided. The study included 94 CF patients homozygous for F508del mutation of CFTR. To compare their' clinical condition, several parameters were recorded, e.g. a unique clinical score: disease severity status (DSS). To analyse the effect of non-CF7X genetic polymorphisms on the clinical course of CF patients, the whole coding region of COX 1 and selected COX2 polymorphisms were analysed. Statistical analysis of genotype-phenotvpe associations revealed a relationship between the heterozygosity status of identified polymorphisms and better lung function. These results mainly concern COX2 polymorphisms: -765G>C and 8473T>C. The COX1 and COX2 polymorphisms reducing COX protein levels had a positive effect on all analysed clinical parameters. This suggests an important role of these genes as protective modifiers of pulmonary disease in CF patients, due to inhibition of arachidonic acid conversion into prostaglandins, which probably reduces the inflammatory process.
W pracy oceniano zmienność fenotypową u 10 linii wsobnych żyta ozimego wyselekcjonowanych z różnych odmian uprawnych i form hodowlanych. Różnice pomiędzy liniami dotyczyły cech związanych z produktywnością roślin: długości źdźbła i kłosa, krzewistości, liczby ziaren z kłosa, masy 1000 ziaren, plonu ziarna z rośliny, intensywności fotosyntezy netto i transpiracji, a także liczby chloroplastów w mezofilu oraz zawartości chlorofilu. Wybrane linie wsobne żyta charakteryzowały się dużym zróżnicowaniem pod względem wymienionych cech i wykazywały wartości skrajne. Spośród analizowanych linii wyróżniała się linia J74, która miała najwyższe wartości dla większości cech (długość kłosa, liczba ziaren z kłosa i roślin, ulistnienie oraz aktywność fotosyntetyczna), co predysponuje ją do zastosowania w hodowli wysokoproduktywnych, heterozyjnych odmian żyta.
Studies were carried out in 2003-2005 on the soil in South-Eastern Poland. The experiment was made by randomised blocks in there replications. The factor of the experiment was the cultivars of sweet potato: Carmen Rubin, Goldstar and White Triumph. Full manuring and uniform mineral fertilization were applied in identical amounts: 100 kg N, 43.6 kg P, 124.5 kg K and 25 t·ha⁻¹ FYM. The reproduction material comprised seedlings produced due to in vitro micropropagation. Samples for qualitative examinations (20 potato tubers of mean size) from three field replications for each plot were collected by standard methods. The differences in darkening of raw and coked sweet potato tubers was conditioned by the phenotypical variability.
Introducing indigenous plants into cultivation and breeding programs in order to obtain new taxa can create the undesirable effects and pose a real threat to natural populations. The main problem of our study was to assess whether the phenotypic characters might allow distinguishing cultivated Convallaria majalis from plants occurring in natural habitats. The range of phenotype variability of Convallaria majalis L. shoots from forests and gardens growing in different edaphic factors was studied in 2005–2006 in north (Gdańsk), central (Warsaw) and south (Cracow) regions of Poland. Ten or eleven forest locations and the same number of garden locations were designated in each region. The soil at the forest sites was poorer in phosphorus and calcium and more acidic than at the garden sites from which the cultivated plants were obtained. Thirty flowering shoots were collected from each study site. The numbers as well as size of flowers, size of racemes and leaves were investigated. The following hypotheses were formulated: (a) due to conscious and unconscious selection, cultivated plants should have more numerous and larger flowers and a longer inflorescence (these characters are most significant for the ornamental appearance of the plant) than plants growing in forests; (b) cultivated plants exhibit less phenotype variability than do natural populations. The forest plants from all the three regions together had, in comparison with garden plants, significantly shorter racemes with fewer flowers, a narrower and shorter perianth, and a longer lower leaf with a narrower and longer leaf blade. In the forest plants raceme length exhibited the highest variability while the lowest was noted in perianth length. More morphological differences were noted between the forest and garden plants than among those from the three different regions. The results obtained indicate that the level of variability in the studied characters was similar in forest and cultivated plants. A high level of variability in some morphological characters of lily of the valley probably stems from the high phenotype plasticity of this plant and the diversified biotope conditions in which it occurs. The significant differences between forest and garden plants could have been related to differences in habitat conditions.
In the paper a classification of 117 genotypes (cultivars and clones) in a strawberry collection from the Research Institute of Pomology and Floriculture in Skierniewice was assessed. The classification of genotypes was based on nine characters including some vegetative attributes as well as those describing fruit yielding and susceptibility to the most important strawberry diseases. Data came from a trial observed for three years (2001-2003). They were arranged in an incomplete two-way classification genotypes by years. Estimates of genotypic values were obtained using the best linear unbiased predictor (BLUP) in a random ANOVA model. Two multivariate methods were used: cluster analysis by the Ward’s procedure (identifying homogenous groups of genotypes) and canonical variate analysis (both detecting characters having the largest discriminant power (relative contribution) in distinguishing the groups and visualisation of multivariate similarities of the identified groups). The first two canonical variables accounted for 87% of the total variation between groups. The first canonical variable was strongly correlated with fruit firmness and fruit size. Therefore these characters have the strongest discriminant power in distinguishing the identified groups.
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