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Fruits of Eugenia jambolana were analysed for total phenolics and antioxidant activity of three successive development stages viz. 1. Mature green 2. Half - ripened and 3. Fully ripened. The total anthocyanin concentrations were higher in fully ripened stage, the total phenolic content was maximum achieved in mature green stage (354 mg L-1). Antioxidant activity also strongly depended on ripening stages, showing completely opposite compared to that of total phenolics. Since total phenolics and antioxidant activity performed nearly “Object and its reflection in the mirror” trend.
The aim of our work was to investigate the antioxidant activity of Rheum palmatum extracts. Antiradical activity against DPPH and ABTS radicals, reducing power FRAP and total phenolic contents, were investigated in one-, two- and three-year-old roots of rhubarb fertilized with nitrogen at the rates of 50, 100 and 200 kg N/ha. It was proved that nitrogen dose as well as the age of plantation did significantly influence antioxidant activity and total phenolic of root extracts. The highest values were determined in one-year-old plants, antioxidant activity ranged the level of 112–203 µM Trolox/g and total phenolic compounds average content was 22 mg GAE/g FW. Two-year-old roots were characterized by 3–11%, and three-year-old ones by 15–23% lower antioxidant activity and phenolic compounds content. When nitrogen dose increased, polyphenols content, DPPH and FRAP values increased as well, although, ABTS showed a different tendency.
Twenty two multifloral honey samples representing central western parts of Nepal were examined spectrophotometrically for their antioxidant properties and total phenol content. The modified Folin-Ciocalteu method was used to determine total phenol content and 2,2-diphenyl-1-picrylhy-drazyl radicals (DPPHO assay for antiradical activity. In all samples, physicochemical parameters like moisture, reducing sugar, sucrose, ash, free acidity and water insoluble matter were also measured according to harmonized methods of the International Honey Commission (IHC). The results of physicochemical analysis showed that all the values, except for moisture of a small number of high altitude honey samples, are in good agreement with the current Nepalese standard. The total phenolic contents of honey, collected from high and low altitude, ranged from 154.87 to 41.90 mg gallic acid equivalent (GAE/100 g) respectively, at corresponding antiradical activity using DPPH expressed as percent inhibition of 76.66% and 25.69%. The IC50 values of selected high altitude honey samples ranged from 56 to 72 mg/mL. The total antioxidant properties were correlated (P<0.01) between total phenol content and antiradical activity (r=0.992). The obtained results demonstrate that the Nepalese honey collected from high altitude region contained more antioxidants than honey of low altitude region.
Globe artichoke (Cynara cardunculus L. var. scolymus (L.) Fiori) is an edible herbaceous perennial plant that plays an important economic role in Mediterranean agriculture. In recent years, extensive research, which aimed to characterize the phenolic profile of the most important globe artichoke cultivars in Italy, has been conducted. However, very little information is available on the phenolic composition of cultivars traditionally grown in Spain. In this work, six cultivars (‘Opal’, ‘Symphony’, ‘Concerto’, ‘Madrigal’, ‘Blanca de Tudela’ and ‘A-106’) cultured in Spain were characterized according to their phenolic content. The phenolic profile differed between cultivars, and also between flower parts. The major phenolic compound in all the different cultivars was chlorogenic acid. Of the six studied cultivars, ‘Madrigal’ had the highest phenolic content.
The content and chemical composition of phenolic compounds in above- and underground organs of dropwort during second year of plant vegetation were studied. Five flavonoids (hyperosid, astragalin, spireaoside, kaempferol, quercetin), 2 catechin derivatives ((+)-catechin, (-)-epigallocatechin), and 7 polyphenolic acids (ellagic, gallic, syringic, salicylic, chlorogenic, caffeic and rosmarinic) were identified in aboveground organs. Their content, both in flowers and leaves, was significantly higher at the beginning of flowering as compared with full flowering stage. In underground organs (+)-catechin and its derivatives ((-)-epigallocatechin, (-)-epigallocatechin gallate, (-)-epicatechin) as well as 2 polyphenolic acids (ellagic and gallic) were identified. Their content was not closely related to the stage of plant development.
Thirty two samples of different types of Polish honeys were investigated in order to assess their total phenolic content by Folin-Ciocalteau method and their potential antioxidant activity by 1,1 diphenyl-2-picrylhydrazyl (DPPH) and ABTS methods. Results of the study showed that the total phenolic content and antioxidant activity differed widely among different honey types. The highest phenolic content and antioxidant activity had dark honeys, while the lowest antioxidant activity and phenolic content were found in pale honeys. Statistical analysis showed that the total phenolic content of honey was correlated with its antioxidant activity.
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