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Studies carried out in 2003–2005 included determinations of free phenolic acids content in edible parts (shoots, pseudostem) of onion, shallot, and Welsh onion grown for green bunching in the field and forced in the greenhouse. Plants for phenolic acids contents analyses were achieved from setting the small bulbs (common onion, shallot) or annual plants from the seedling setting in the case of Welsh onion. In the field cultivation, plants were grown under short-term covers made of perforated PE film and non-woven PP, while forcing was realized in heated and unheated greenhouse. Free phenolic acids contents were determined after plant harvest, when plants reached the size useful for trading in bunches. Phenolic acids contents were determined in shoots and pseudostem by means of spectrometric Arnova method with recalculation onto caffeic acid. Performed study revealed that shoots contained significantly more free phenolic acids as compared to the pseudostem. Referring to the field cultivation, the component concentration in shoots was 0.23 mg·100 g⁻¹ FW, whereas due to forcing 0.135 mg·100 g⁻¹ FW, on average. Contents of phenolic acids at pseudostems of studied plants from the field cultivation were 0.05 mg·100 g⁻¹ FW, while at forced plants 0.04 mg·100 g⁻¹ FW. No significant differences related to the level of phenolic acids between common onion and shallot were found, both in shoots and pseudostem. However, shoots of Welsh onion contained significantly less phenolic acids, regardless the cultivation place and cover application. The accelerated field cultivation resulted in shoots of Welsh onion was 0.19 mg·100 g⁻¹ FW phenolic acids in FW, whereas the greenhouse forcing 0.11 mg·100 g⁻¹ FW. Regardless the species, forced plants contained considerably less phenolic acids (namely in shoots) as compared to plants grown in the field.
Chemical analyses aiming the examination of free phenolic acids content (re-count to the caffeic acid) in the seeds of 12 bean cultivars of Phaseolus vulgaris L. and Phaseolus coccineus L. were carried out in years 2002-2004 in the Department of Vegetable Crops and Medici­nal Plants in Agricultural University, Lublin. The content of phenolic acids in seeds taken from the bean cultivars of white and colour bean seed coat was compared. The content of phenolic acids in seeds, bean seed coat and seed cotyledons was also determined. As a result of the experiment, a significant difference in content of free phenolic acids in the seeds of particular bean cultivars was observed. An average content of phenolic acids (re-count to caffeic acid) was highest in bean seed coat: 0.04% (white seeds) and 0.12% (co­lour seeds: claret-coloured, navy blue and black). The content of phenolic acids in whole bean seeds was 0.05% (white seeds) and 0.07% (colour seeds), but in cotyledonos 0.03% and 0.09%, on average. Bean seed coat was 8.7-11.4% of the whole mass of seeds, depending on the cultivar. The one seed mass was different for Phaseolus vulgaris L. (mean 0.28-0.65 g) and Phaseolus coccineus L. (mean 1.06-1.77 g).
Simultaneous identification of eight phenolic acids (gallic, protocatechuic, chlorogenic, p-hydroxybenzoic, vanillic, caffeic, p-coumaric and ferulic) and three flavonoid aglycones (Iuteolin, kaempferol and apigenin) in Et20-fractions of inflorescences and leaves of five species of Cirsium genus - C. arvense, C. oleraceum, C. palustre, C. rivulare and C. vulgare was carried out by HPLC method. Phenolic acids and flavonoids were analyzed also in terms of quantity. The contents of phenolic acids, expressed as cafifeic acid, determined by the spectroscopic method with Arnov's reagent, were higher in leaves than in flower heads of all investigated species and amounted from 0.15% in C. vulgare inflorescences to 1.31% in C. palustre leaves. The flavonoid contents, indicated by Christ-Miiller's method ranged from 16 mg • 100g1 per dry source in C. arvense and C. palustre inflorescences to 70 mg • 100g ' in C. oleroceum Leaves.
Badania przeprowadzone w latach 2004-2006 obejmowały analizę zawartości flawonoidów (w przeliczeniu na kwercetynę) i kwasów fenolowych (w przeliczeniu na kwas kawowy) w cebulach szalotki. Zawartość tych składników oceniono w łuskach suchych i mięsistych 10. miejscowych populacji i 2. odmian szalotki. Stwierdzono, że niezależnie od populacji lub odmiany, łuski suche zawierały ponad 60 razy więcej flawonoidów (średnio 0,19%). Podobną zależność stwierdzono w odniesieniu do zawartości kwasów fenolowych - łuski suche zawierały ponad 28 razy więcej tego składnika w porównaniu do zawartości w łuskach mięsistych. Spośród badanych populacji i odmian największą zawartością flawonoidów w łuskach suchych i mięsistych wyróżniały się populacje A-081, PV-73, Lubelska oraz odmiana Creation F₁. Poziom zawartości kwasów fenolowych w cebulach szalotki był istotnie zróżnicowany w obu latach badań, a szczególnie zasobne w ten składnik okazały się łuski suche populacji A-20, PV-73, PV-87 oraz odmiany Francuska Półdługa. U tych populacji i odmian, z wyj ątkiem PV-73, również łuski mięsiste zawierały najwięcej kwasów fenolowych. Uzyskane wyniki poziomu zawartości flawonoidów oraz kwasów fenolowych w łuskach suchych i mięsistych szalotki wskazują, że cebula ta jest cennym źródłem powyższych związków. Niektóre populacje charakteryzujące się wysoką ich zawartością mogą stanowić wyjściowy materiał do prac hodowlanych.
Chilling is one of the major abiotic stress which limites yield and quality of many crops. The seedlings of rice varieties namely Koshihikari (Japonica subtype) chilling tolerant, and the susceptible NMR2 (Indica subtype) were treated at 25/15 °C and 5/4 °C day/night to determine the growth parameters, phenolic contents, and antioxidant activity. It was found that in all treatments, the growth of MNR2 including root and shoot lengths, and leaf and root weights were inhibited at greater levels than Koshihikari. There were seven phenolic acids identified in leaves of Koshihikari including caffeic acid, vanillin, ferulic acid, sinapic acid, benzoic acid, ellagic acid, and cinamic acid, but only benzoic acid and ellagic acid were found in leaves of MNR2. In contrast, only vanillic acid and ellagic acid were observed in roots of Koshihikari, whilst ellagic acid and cinnamic acid were found in roots of MNR2. It was found that rice reduced amount of phenolic acids but promoted quantity of total phenolic content (TPC) and total flavonoid content (TFC) and level of antioxidant activity in chilling stress, although the level of responses varied between Japonica and Indica subtypes. Tolerant rice possessed greater bound flavonoids, phenolics and phenolic acids, but susceptible rice accumulated greater free TPC and TFC in reduced temperature. Findings of this study highlighted that phenolic constituents in bound forms of phenols, polyphenols, and flavonoids may play an active role in rice plants than phenolic acids under chilling stress but need further elaboration.
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