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1

Effect of beta-1,3-1,6-D-glucan on the phagocytic activity and oxidative metabolism of pripheral blood granulocytes and monocytes in rats

100%
Wojcik R., Janowska E., Malaczewska J., Siwicki A. K.
Bulletin of the Veterinary Institute in Puławy
|
2009
|
tom 53
|
nr 2
241-246
The experiment was performed on 20 adult Wistar rats aged 12 weeks, divided into two equal groups (control and experimental), each comprised of five males and five females. From the first day of the experiment, the experimental group rats were fed Murigran feed supplemented with ß-l,3/l,6-D-glucan at a dosage of 12-19 mg/rat/d, subject to body weight, while the control- group was administered the same feed without any additives. At the beginning of the experiment and then after 14 days, arterial blood samples were collected from the rats and diluted with heparin to measure and compare the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes by flow cytometry. Statistically higher levels of the activity were observed in the group of rats administered glucan than in controls, expressed in terms of the percentage of phagocytic cells as well as average fluorescence intensity. ß-l,3/l,6-D-glucan also had a positive effect on the oxidative metabolism of both granulocytes and monocytes after stimulation with E. coli, and on the oxidative metabolism of granulocytes after stimulation with PMA.
2
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Content available

Effect of conglutinin on phagocytic activity of bovine granulocytes

100%
Dec M., Wernicki A., Puchalski A., Urban-Chmiel R., Radej S.
Polish Journal of Veterinary Sciences
|
2012
|
tom 15
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nr 3
In the present study we investigated the effect of bovine conglutinin on the phagocytic activity of leukocytes. We measured both the chemotactic activity of conglutinin and its effect on the internalization of zymosan particles and E. coli by granulocytes. We also assessed the binding of conglutinin to various microorganisms isolated from clinical cases in cattle. We showed that conglutinin binds strongly to the surface of yeast cells and to mannan-rich zymosan particles, while weak binding was observed in the case of the bacterial strains tested, including those whose O antigen is composed of mannan. Conglutinin (1-10 μg/ml) neither acts as a chemotactic factor for peripheral blood leukocytes nor affects ingestion of E. coli by granulocytes. However, as flow cytometry based assay showed, conglutinin (0.1-1 μg/ml) increased ingestion of zymosan expressed as mean fluorescence intensity (MFI) of positive cells.
3

Morphology and phagocytic activity of heterophilic granulocytes in the rat and quail

100%
Kaczanowska-Taraszkiewicz E., Gromysz-Kalkowska K.
Zoologica Poloniae
|
1997
|
tom 42
|
nr 1-4
4

Polymyxin B, gentamycin and neomycin inhibit phagocytic activity of Tetrahymena pyriformis

84%
Krawczynska W.
Acta Protozoologica
|
1990
|
tom 29
|
nr 3
5

Cell-mediated immunity in yellow forms in rainbow trout

84%
Siwicki A., Dobosz S., Goryczko K., Kuzminski H., Kohlmann K., Trapkowska S., Kazun B.
Polish Journal of Veterinary Sciences
|
2003
|
tom 06
|
nr 3 Suppl.
6

Influence of naturally acquired feline leukemia virus (FeLV) infection on the phagocytic and respiratory burst activity of neutrophils and monocytes of peripheral blood

84%
Hoffmann-Jagielska M., Winnicka A., Jagielski D., Micun J., Zmudzka M., Lechowski R.
Polish Journal of Veterinary Sciences
|
2005
|
tom 08
|
nr 2
7

The influence of liposomes containing cholesterol on phagocytic activity and proliferation of Tetrahymena cells

84%
Fabczak H.
Acta Protozoologica
|
1988
|
tom 27
|
nr 2
8

High L-ascorbic acid content of diet modulates the non-specific immunity in rats

84%
Niemiec T., Sawosz E., Grodzik M., Chwalibog A.
Animal Science Papers and Reports
|
2009
|
tom 27
|
nr 2
149-157
This study was designed to examine the effect of high dose of L-ascorbic acid (L-AA) as a dietary supplement for rats on the phagocytic activity and oxidative burst of non-specific immune cells. Thirty Wistar rats (225-245 g initial body weight) were divided into three groups fed diet supplemented with 0.0, 0.3, and 0.6% L-AA (0, 187 and 375 mg L-AA/kg feed) for 41 days. At the end of the experiment, blood samples were analysed for selected indicators of non-specific immunity. The application of 0.6% of LAA, as compared to the remaining two groups reduced phagocytic activity measured as a number of opsonized E. coli cells consumed by neutrophiles and monocytes. However, 0.6% of dietary AA supplementation led to increased phagocytic monocytes and neutrophiles percentage,which produced reactive oxygen species after stimulation with opsonized bacteria (E. coli), phorbol 12-myristate 13-acetate (PMA) and the chemotactic peptide N-formyl-Met-Leu-Phe (fMLP).These results indicate that megadose (0.6%) of dietary AA can reduce an oxygen-independent phagocytosis, but increase the number of neutrophiles and monocytes representing the oxygendependent mechanism of killing.
9

Study of insulin-provoked endocytosis in different tava of Tetrahymena

84%
Kovacs P., Csaba G.
Acta Protozoologica
|
1990
|
tom 29
|
nr 3
10

A note on the effect of feeding genetically modified potatoes on selected indices of non-specific resistance in rats

84%
Winnicka A., Sawosz E., Klucinski W., Kosieradzka I., Szopa J., Malepszy S., Pastuszewska B.
Journal of Animal and Feed Sciences. Supplement
|
2001
|
tom 10
|
nr 2
11

Effect of cardiopulmonary bypass on neutrophil activity in pediatric open-heart surgery

84%
Pasnik J., Siniewicz K., Moll J. A., Moll J., Baj Z., Sysa A., Zeman K.
Archivum Immunologiae et Therapiae Experimentalis
|
2005
|
tom 53
|
nr 3
12
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Content available

Effect of Biolex Beta-HP on phagocytic activity and oxidative metabolism of peripherial blood granulocytes and monocytes in rats intoxicated by cyclophosphamide

67%
Wojcik R.
Polish Journal of Veterinary Sciences
|
2010
|
tom 13
|
nr 1
181-188
The objective of this study was to determine the effect of ß-l,3/l,6-D-glucan (Biolex-Beta HP) on the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in rats intoxicated by cyclophosphamide. The experimental material comprised 40 adult Wistar rats aged 14 weeks, divided into two equal groups, a control group and an experimental group, each of 20 adult rats, including 10 males and 10 females. In the course of 3 successive days, 20 rats from the experimental group were administered cyclophosphamide intramuscularly at a dose of 50 mg/kg body weight/day. On the 8th day of the experiment, 10 control group (K) rats and 10 experimental group (C) rats were sacrificed. Arterial blood samples were collected and diluted with heparin to determine and compare the phagocytic activity (Phatogest) and oxidative metabolism (Bursttest) of peripheral blood granulocytes and monocytes by flow cytometry. Starting on the 8lh day of the experiment, the feed of the remaining 10 rats from the experimental group (C+G) and 10 rats from the control group (G) was supplemented with Biolex-Beta HP at a dose of 50 mg/kg body weight/day for 14 consecutive days. On day 22, arterial blood samples were collected from all (C+G) and (C) group rats, diluted with heparin to determine and compare the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes by flow cytometry. The results showed that Biolex-Beta HP modulated the phagocytic activity and oxidative metabolism of blood neutrophils and monocytes suppressed by cyclophosphamide in rats. The immunorestoring activity of Biolex-Beta HP was observed in this study.
13

Wplyw preparatow dwuskladnikowych na aktywnosc granulocytow krwi obwodowej szczurow

51%
Latuszynska J., Luty S., Przylepa E., Haratym-Maj A.
Bromatologia i Chemia Toksykologiczna
|
2000
|
tom 33
|
nr 1
23-28
Zbadano wpływ dwóch pestycydów (Decis Prime 415 EC i Nurelle D 550 EC) podawanych naskórnie na aktywność fagocytarną i bakteriobójczą neutrofili krwi obwodowej. Obydwa preparaty wpływają stymulujące na aktywność fagocytarną i bakteriobójczą neutrofili.
14

Effects of immunomodulator FK-156 applied in lambs during the postnatal life

51%
Czarnecki A., Rybka A., Studzinski T., Winiarczyk S.
Advances in Agricultural Sciences
|
1992
|
tom 01
|
nr 1
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