Wyniki wyszukiwania

1

Peroxynitrite-induced hemolysis of human erythrocytes and its possible mechanism

100%

Soszynski M., Klimczak A., Grzelak A., Bartosz G., Zylinska L.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

2

Does the peroxidase-like activity of sodium dodecyl sulphate-modified cytochrome c increase after peroxynitrite or radiation treatment?

100%

Gebicka L., Didik J.

Acta Biochimica Polonica

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2005

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tom 52

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nr 2

The peroxidase-like activity of cytochrome c is considerably increased by unfolding of the protein. The enhancement of the activity is due to the higher reaction rate of unfolded cytochrome c with hydrogen peroxide, which is the rate-determining step in the peroxidase cycle of cytochrome c (Gębicka, L., 2001, Res Chem Intermed 27, 717–23). In this study we checked whether combined action of two unfolding factors, SDS and peroxynitrite or radiation (hydroxyl radicals), increases the peroxidase-like activity of cytochrome c more than any single treatment alone. Peroxynitrite reacts with SDS-modified cytochrome c in the same way as with native cytochrome c, via intermediate radical products, •OH/•NO2, arising from peroxynitrite homolysis. We found that SDS-modified cytochrome c is much more sensitive to oxidative damage than the native protein. Partial unfolding of cytochrome c by SDS causes the peroxide substrate to have a better access to the heme center. On the other hand, the amino acids located in the vicinity of the active site and/or heme group become accessible for oxidizing radicals. The overall effect observed is that the peroxidase-like activity of SDS-modified cytochrome c decreases with an increase of the concentration of the oxidizing species (peroxynitrite or radiolytically generated hydroxyl radicals). The damage of SDS-modified cytochrome c caused by irradiation is much more significant than that observed after peroxynitrite treatment.

3

The effect of peroxynitrite and some antioxidants on the rate of osmotic hemolysis of erythrocytes

100%

Wrobel A., Lukaszynska B., Kedzierska J.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

4

Biochemical targets of nitric oxide-induced toxicity

100%

Grudzinski I. P.

Roczniki Państwowego Zakładu Higieny

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2003

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tom 54

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nr 1

Nitric oxide (NO) has become one of the most intensively studied molecules in recent years. Although its beneficial role has been well established, a large body of adverse effects was also attributed to NO and/or its red-ox derivatives in biological systems. Peroxynitrite (ONO-), a product of reaction between NO and superoxide anion (O2•-) was recognized as a potent pro-oxidant endogenous toxicant. The agent was found to induce DNA and protein oxidative damages leading to increased risk(s) of severe human pathologies including cancer. In this review, the discrete chemical aspects of both nitric oxide and peroxynitrite have been discussed in an attempt to elucidate the major biochemical target(s) of NO-and/or peroxynitrite-induced toxicity.

5

Decrease in 2,2,6,6-tetramethyl-piperidine-1-oxyl [TEMPO] EPR signal in peroxynitrite-treated erythrocyte membranes

100%

Wrobel A.

Cellular and Molecular Biology Letters

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2001

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tom 06

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nr 2

6

The role of reactive nitrogen species and cigarette smoke in activation of transcription factor NF-kappaB and implication to inflammatory processes

84%

Bar-Shai M., Hasnis E., Wiener-Megnazi Z., Reznick A. Z.

Journal of Physiology and Pharmacology. Supplement

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2006

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tom 57

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nr 4

39-44

Using the electromobility shift assay (EMSA) in the rat myoblast system, the activation of transcription factor NF-B by reactive nitrogen species was evaluated. Two distinct patterns of activation were demonstrated. Whereas NO donor, SNAP, activated NF-B in the classical pathway, which led to a transient response, NF-B activation by peroxynitrite donor, SIN-1, was mediated by an alternative pathway, which has been demonstrated in previous works to involve tyrosine nitration of the NF-B inhibitory protein I-Balpha. This led to a constitutive non-transient activation of NF-B and a prolonged inflammatory reaction. Lymphocytes exposed to mild intensity of cigarette smoke for 8 h, which activated NF-B, exhibited a decrease in the fraction of apoptotic cells from 27% to 19% compared with lymphocytes exposed to atmospheric air, using the FACS Annexin V assay. This also has been shown in previous works to be mediated by peroxynitrite. Thus, mild exposure to cigarette smoke induces NF-B activation, which can attenuate apoptosis in human lymphocytes and lead to prolonged inflammatory response. A possible proposed mechanism for induction of chronic inflammatory response may involve peroxynitrite-induced activation of NF-B.

7

Atorvastatin enhanced nitric oxide release and reduced blood pressure, nitroxidative stress and RANTES levels in hypertensive rats with diabetes

84%

Mason R. P., Corbalan J. J., Jacob R. F., Dawoud H., Malinski T.

Journal of Physiology and Pharmacology

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2015

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tom 66

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nr 1

8

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The reactive nitrogen species affect potato immunity to Phytophthora infestans

84%

Arasimowicz-Jelonek M., Floryszak-Wieczorek J., Abramowski D., Izbianska K.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

9

The antioxidant properties of carnitine in vitro

84%

Solarska K., Lewinska A., Karowicz-Bilinska A., Bartosz G.

Cellular and Molecular Biology Letters

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2010

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tom 15

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nr 1

90-97

Many of the effects of carnitine are ascribed to its antioxidant properties. The aim of this study was to evaluate the antioxidant properties of carnitine in vitro. Carnitine was found to decolorize ABTS•+, and to protect fluorescein against bleaching induced by AAPH-derived peroxyl radicals and peroxynitrite, thiol groups against oxidation induced by hydrogen peroxide, peroxyl radicals, hypochlorite and peroxynitrite, and erythrocytes against hemolysis induced by peroxyl radicals and hypochlorite. These results show that carnitine has a direct antioxidant action against physiologically relevant oxidants.

10

Lipid peroxidation, reactive oxygen species and antioxidative factors in the pathogenesis of gastric mucosal lesions and mechanism of protection against oxidative stress - induced gastric injury

84%

Kwiecien S., Jasnos K., Magierowski M., Sliwowski Z., Pajdo R., Brzozowski B., Mach T., Wojcik D., Brzozowski T.

Journal of Physiology and Pharmacology

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2014

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tom 65

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nr 5

11

Time-dependent supplementation of vitamin E influences leptin expression in the aortic layers of rats fed atherogenic diet

84%

Krawczynska A., Olczak E., Rembiszewska A., Herman A. P., Gromadzka-Ostrowska J.

Journal of Physiology and Pharmacology

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2014

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tom 65

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nr 1

12

Peroxynitrite mediator of the toxic action of nitric oxide

84%

Bartosz G.

Acta Biochimica Polonica

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1996

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tom 43

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nr 4

Peroxynitrite (oxoperoxonitrate(-l)), anion of peroxynitrous acid, is thought to mediate the toxic action of nitric oxide and superoxide anion. Peroxynitrite is formed in a fast reaction between these species, reacts with all classes of biomolecules, is cytotoxic, and is thought to be involved in many pathological phenomena. Its main reactions involve one- and two-electron oxidation and nitration. Protein nitration is often used as a footprint of peroxynitrite reactions in vivo. Nitration of tyrosine and of tyrosyl residues in proteins may be an important mechanism of derangement of biochemical signal transduction by this compound. However, apparently beneficial effects of peroxynitrite have also been described, among them formation of nitric oxide and nitric oxide donors in reactions of peroxynitrite with thiols and alcohols.

13

Endothelial NADH-NADPH-dependent enzymatic sources of superoxide production: relationship to endothelial dysfunction

84%

Kalinowski L., Malinski T.

Acta Biochimica Polonica

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2004

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tom 51

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nr 2

There is growing evidence that endothelial dysfunction, which is often defined as the decreased endothelial-derived nitric oxide (NO) bioavailability, is a crucial factor leading to vascular disease states such as hypertension, diabetes, atherosclerosis, heart failure and cigarette smoking. This is due to the fact that the lack of NO in en- dothelium-dependent vascular disorders contributes to impaired vascular relaxation, platelet aggregation, increased vascular smooth muscle proliferation, and enhanced leukocyte adhesion to the endothelium. During the last several years, it has become clear that reduction of NO bioavailability in the endothelium-impaired function disorders is associated with an increase in endothelial production of superoxide (O2 ). Because O2 - rapidly scavenges NO within the endothelium, a reduction of bioactive NO might occur despite an increased NO generation. Among many enzymatic systems that are capable of producing O2 -, NAD(P)H oxidase and uncoupled endothelial NO synthase (eNOS) apparently are the main sources of O2- in the endothelialcells. It seems that O2– generated by NAD(P)H oxidase may trigger eNOS uncoupling and contribute to the endothelial balance between NO and O2–. That is maintained at diverse levels.

14

Nitrite mitigates lipid peroxidation in rat gastric mucosa

84%

Grudzinski I. P., Frankiewicz-Jozko A., Szarska E.

Roczniki Państwowego Zakładu Higieny

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2005

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tom 56

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nr 1

Sodium nitrite, a nitric oxide (NO) donor in the acid pH, has been found to mitigate lipid peroxidation in rat gastric mucosa, and it elevated both Cu, Zn- and Mn-forms of the superoxide dismutase in this tissue. Putrescine, a simple p oly amin e of anti-oxidant properties has been shown to normalize lipid peroxidation levels in nitrite-treated animals.

15

The effect of peroxynitrite and some antioxidants on the rate of osmotic hemolysis of bovine erythrocytes

84%

Wrobel A., Lukaszynska B., Kedzierska J.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 2

Bovine erythrocytes treated with peroxynitrite (ONOO-), a cytotoxic species formed in vivo via the reaction of nitric oxide (NO*) and the superoxide anion (O2-*), show an increased rate of hemolysis on sudden osmotic stress. The increase in the rate was peroxynitrite concentration dependent. In the presence of some antioxidants (uric acid, ascorbic acid, glutathione, melatonin and albumin), this effect was significantly lower, with ascorbic acid as the most efficient antioxidant.

16

Studies on antioxidant properties of polyphenol-rich extract from berries of Aronia melanocarpa in blood platelets

67%

Olas B., Wachowicz B., Nowak P., Kedzierska M., Tomczak A., Stochmal A., Oleszek W., Jeziorski A., Piekarski J.

Journal of Physiology and Pharmacology

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2008

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tom 59

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nr 4

823-835

The antioxidant properties of extract from berries of Aronia melanocarpa (chokeberry) containing: anthocyanidines, phenolic acids and quercetine glycosides on oxidative/nitrative stress induced by peroxynitrite (ONOO-, a powerful physiological oxidant, nitrating species and inflammatory mediator) in human blood platelets were studied in vitro. The extract from A. melanocarpa (5 – 50 µg/mL) significantly inhibited platelet protein carbonylation (measured by ELISA method) and thiol oxidation estimated with 5,5’-dithio-bis(2-nitro-benzoic acid) (DTNB) induced by peroxynitrite (0.1 mM) (IC50 - 35 µg/mL for protein carbonylation, and IC50 - 33 µg/mL for protein thiol oxidation). The tested extract only slightly reduced platelet protein nitration (measured by C- ELISA method). The extract also caused a distinct reduction of platelet lipid peroxidation induced by peroxynitrite. Moreover, in our preliminary experiments we observed that the extract (50 µg/mL) reduced oxidative/nitrative stress in blood platelets from patients with breast cancer. The obtained results indicate that in vitro the extract from A. melanocarpa has the protective effects against peroxynitrite – induced oxidative/nitrative damage to the human platelet proteins and lipids. The extract from A. melanocarpa seems to be also useful as an antioxidant in patients with breast cancer.

17

Mechanisms underlying cigarette smoke-induced NF-kappaB activation in human lymphocytes: the role of reactive nitrogen species

67%

Hasnis E., Bar-Shai M., Burbea Z., Reznick A. Z.

Journal of Physiology and Pharmacology. Supplement

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2007

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tom 58

|

nr 5

18

Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)

67%

Olchowik E., Lotkowski K., Mavlyanov S., Abdullajanova N., Ionov M., Bryszewska M., Zamaraeva M.

Cellular and Molecular Biology Letters

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2012

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tom 17

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nr 3

Erythrocytes are constantly exposed to ROS due to their function in the organism. High tension of oxygen, presence of hemoglobin iron and high concentration of polyunsaturated fatty acids in membrane make erythrocytes especially susceptible to oxidative stress. A comparison of the antioxidant activities of polyphenol-rich plant extracts containing hydrolysable tannins from sumac leaves (Rhus typhina L.) and condensed tannins from grape seeds (Vitis vinifera L.) showed that at the 5–50 μg/ml concentration range they reduced to the same extent hemolysis and glutathione, lipid and hemoglobin oxidation induced by erythrocyte treatment with 400 μM ONOO− or 1 mM HClO. However, extract (condensed tannins) from grape seeds in comparison with extract (hydrolysable tannins) from sumac leaves stabilized erythrocytes in hypotonic NaCl solutions weakly. Our data indicate that both hydrolysable and condensed tannins significantly decrease the fluidity of the surface of erythrocyte membranes but the effect of hydrolysable ones was more profound. In conclusion, our results indicate that extracts from sumac leaves (hydrolysable tannins) and grape seeds (condensed tannins) are very effective protectors against oxidative damage in erythrocytes.

19

Antioxidant properties of PF9601N, a novel MAO-B inhibitor: assessment of its ability to interact with reactive nitrogen species

67%

Bellik L., Dragoni S., Pessina F., Sanz E., Unzeta M., Valoti M.

Acta Biochimica Polonica

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2010

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tom 57

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nr 2

The novel MAO-B inhibitor PF9601N, its cytochrome P450-dependent metabolite FA72 and l-deprenyl were studied as potential peroxynitrite (ONOO-) scavengers and nitric oxide synthase (NOS) inhibitors. The scavenging activity of these compounds was evaluated by measuring the oxygen consumption through peroxynitrite-mediated oxidation of both linoleic acid and brain homogenate. FA72, PF9601N and l-deprenyl caused a concentration-dependent inhibition of ONOO--induced linoleic acid oxidation with an IC50 value of 60.2 μM, 82.8 μM and 235.8 μM, respectively. FA72 was the most potent also in inhibiting ONOO--induced brain homogenate oxidation with an IC50 value of 99.4 μM, while PF9601N and l-deprenyl resulted weaker inhibitors in the same experimental model, showing an IC50 value of 164.8 and 112.0 μM, respectively. Furthermore, both the novel MAO-B inhibitor as well as its metabolite were able to strongly inhibit rat brain neuronal NOS (IC50 of 183 μM and 192 μM, respectively), while l-deprenyl at the highest concentration used (3 mM), caused only a slight decrease of the enzyme activity. Moreover, inducible NOS was strongly inhibited by FA72 only. All these results suggest that PF9601N could be a promising therapeutic agent in neurodegenerative disorders such as Parkinson's disease.

20

Reactive oxygen species [ROS] and reactive nitrogen species [RNS] alter metabolic effect of insulin in rat L6 satellite cells

67%

Orzechowski A., Grzelkowska K.

Polish Journal of Veterinary Sciences

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2000

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tom 03

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nr 1

This study investigates the effect of superoxide anion radical (02); hydrogen peroxide (H202), nitric oxide (NO) and peroxynitrite (ONOO), which often accompany inflamed, endotoxic or exercised muscle on insulin action in DTsatellite cells. In order to induce quiescence, rat L6 myoblasts were subjected to transition from G2/M to Gl phase by the application of serum-reduced medium. Insulin stimulating effect on cell mitogenicity and anabolism was dose-dependent and hormetic. Application of H202 alone enhanced protein synthesis with dose-dependency but had no effect on mitogenicity. While insulin and H202 were used together, (i.e. at low H202 dose) insulin action was not affected regardless of the combination used, except the loss of dose- dependency on protein synthesis, but for 100 μM of H202 insulin action ceased abruptly and totally. Since there were no additive effects of both factors, we conclude that H202 may contribute to the insulin-induced anabolic reaction, however, below 100 The application of 02- donor stimulated protein synthesis and slightly inhibited [cell proliferation] though dose-response pattern was not observed suggesting apparent limitations to 02- diffusion into the cell. Moreover 02- inhibited both insulin-enhanced mitogenicity and protein synthesis by abrogating dose-response fashion of insulin action. The introduction of NO and ONOO- donors alone to control systems inhibited cell proliferation in a dose-dependent manner having no effect on protein synthesis (except the low doses of SIN-1). Insulin-stimulated syntheses of both DNA and protein were inhibited in a dose- dependent manner by SIN-1 (NO and 02' donor). In the presence of SNP (NO donor) mitogenic effect of insulin was abolished whereas protein synthesis was diminished only by the highest SNP concentration used (0.5 mM). Taken together, these results have shown that hydrogen peroxide (H202), nitric oxide (NO) and peroxynitrite (ONOO ) provide a good explanation for developing resistance to growth promoting activity of insulin in satellite cells under conditions of oxidant stress.

Ograniczanie wyników

Peroxynitrite-induced hemolysis of human erythrocytes and its possible mechanism

Does the peroxidase-like activity of sodium dodecyl sulphate-modified cytochrome c increase after peroxynitrite or radiation treatment?

The effect of peroxynitrite and some antioxidants on the rate of osmotic hemolysis of erythrocytes

Biochemical targets of nitric oxide-induced toxicity

Decrease in 2,2,6,6-tetramethyl-piperidine-1-oxyl [TEMPO] EPR signal in peroxynitrite-treated erythrocyte membranes

The role of reactive nitrogen species and cigarette smoke in activation of transcription factor NF-kappaB and implication to inflammatory processes

Atorvastatin enhanced nitric oxide release and reduced blood pressure, nitroxidative stress and RANTES levels in hypertensive rats with diabetes

The reactive nitrogen species affect potato immunity to Phytophthora infestans

The antioxidant properties of carnitine in vitro

Lipid peroxidation, reactive oxygen species and antioxidative factors in the pathogenesis of gastric mucosal lesions and mechanism of protection against oxidative stress - induced gastric injury

Time-dependent supplementation of vitamin E influences leptin expression in the aortic layers of rats fed atherogenic diet

Peroxynitrite mediator of the toxic action of nitric oxide

Endothelial NADH-NADPH-dependent enzymatic sources of superoxide production: relationship to endothelial dysfunction

Nitrite mitigates lipid peroxidation in rat gastric mucosa

The effect of peroxynitrite and some antioxidants on the rate of osmotic hemolysis of bovine erythrocytes

Studies on antioxidant properties of polyphenol-rich extract from berries of Aronia melanocarpa in blood platelets

Mechanisms underlying cigarette smoke-induced NF-kappaB activation in human lymphocytes: the role of reactive nitrogen species

Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.)

Antioxidant properties of PF9601N, a novel MAO-B inhibitor: assessment of its ability to interact with reactive nitrogen species

Reactive oxygen species [ROS] and reactive nitrogen species [RNS] alter metabolic effect of insulin in rat L6 satellite cells