Wyniki wyszukiwania

1

Spectral properties of phthalocyanines incorporated into resting and stimulated human peripheral blood cells

100%

Waszkowiak A., Frackowiak D., Wiktorowicz K., Miyake J.

Acta Biochimica Polonica

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2002

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tom 49

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nr 3

Human peripheral blood cells stimulated by phytohemagglutinin (which serve as a model of cancerous cells) and resting cells were incubated in dimethyl sulfoxide solutions of various phthalocyanines. In order to diminish the influence of atmospheric oxygen the cells were embedded in a polymer (polyvinyl alcohol) film. Fluorescence spectra of the samples were measured over two regions of excitation wavelengths: at 405 nm (predominant absorption of the cell material) and in the regions of strong absorption of phthalocyanines (at about 605 nm and 337 nm). The intrinsic emission of cell material became changed as a result both of cells' stimulation and of incubation of cells in dye solution. In most cases the stimulated cells when stained by dye exhibited higher long wavelength fluorescence intensity than resting cells. This suggests higher efficiency of dye incorporation into cancerous cells than into healthy cells. The absorption spectra of samples were also measured. The spectra of various phthalo- cyanines in incubation solvent, in polymer and in the cells embedded in polymer, were compared. The comparison of properties of the cells stimulated for different time periods enabled to establish the conditions of stimulation creating a population of cells incorporating a large number of sensitizing molecules.

2

Selected peripheral blood cell parameters in twelve inbred strains of laboratory mice

84%

Wirth-Dzieciolowska E., Karaszewska J., Pysniak K., Smolinska M., Gajewska M.

Animal Science Papers and Reports

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2009

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tom 27

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nr 1

69-77

In 230 mice from 12 inbred strains (A.CA/W, AKR/W, BALB/cW, BN/aW, CBA/W, CBA-T6/W,C3H/W, C57BL/6W, C57BL/10W, DBA2/W, 129S1/SvW, HLB219/J) the following parameters were measured: the total number of white (WBC) and red (RBC) blood cells, haemoglobin concentration (HGB), haematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) and platelets (PLT) number. Highly significant interstrain differences were shown within RBC, HGB, HCT, MCV and PLT.

3

Effect of granulocyte-macrophage colony-stimulating growth factor on interferon and tumor necrosis factor production in whole blood cell cultures of patients with acute myelogenous leukemia

84%

Kaminska T., Hus I., Dmoszynska A., Kandefer-Szerszen M.

Archivum Immunologiae et Therapiae Experimentalis. Supplement

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2001

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tom 49

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nr 2

83-87

4

Gene expression profiling in peripheral blood nuclear cells in patients with refractory ischaemic end-stage heart failure

84%

Szmit S., Jank M., Maciejewski H., Grabowski M., Glowczynska R., Majewska A., Filipiak K. J., Motyl T., Opolski G.

Journal of Applied Genetics

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2010

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tom 51

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nr 3

Functional analysis of up- and down-regulated genes might reveal whether peripheral blood cells may be considered as a material of diagnostic or prognostic value in patients with end-stage heart failure (HF). The aim of the present study was to compare the transcriptomic profile of peripheral blood nuclear cells from 6 male patients with ischaemic end-stage HF with those of 6 male patients with asymptomatic cardiac dysfunction. The expression of genes in peripheral blood nuclear cells in both groups of patients was measured using whole-genome oligonucleotide microarrays utilizing 35 035 oligonucleotide probes. Microarray analyses revealed 130 down-regulated genes and 15 up-regulated genes in the patients with end-stage HF. Some of the down-regulated genes belonged to the pathways that other studies have shown to be down-regulated in cardiomyopathy. We also identified up-regulated genes that have been correlated with HF severity (CXCL16) and genes involved in the regulation of expression of platelet activation factor receptor (PTAFR, RBPSUH, MCC, and PSMA7). In conclusion, the identification of genes that are differentially expressed in peripheral blood nuclear cells of patients with HF supports the suggestion that this diagnostic approach may be useful in searching for the molecular predisposition for development of severe refractory HF in patients with post-infarction asymptomatic abnormalities and remodelling of the left ventricle. These results need further investigation and validation.

5

Functional eicosanoid test and typing [FET] of peripheral blood cells in eicosanoid related diseases

84%

Schafer D., Baenkler H. W.

Journal of Physiology and Pharmacology. Supplement

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2005

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tom 56

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nr 5

103-118

Monitoring of eicosanoid synthesis in peripheral blood cells has significant potential for improving the diagnosis and therapy of many human diseases. The quantitative relation between concentrations of prostaglandins and leukotrienes is central to the physiologic function of the eicosanoid network. Here we show that this regulation, which we call the functional eicosanoid typing (FET), fluctuates dynamically in individual living blood cells from patients, thereby limiting the accuracy with which concentration circuits of eicosanoids can transfer metabolic information. Using living cells in functional cell testing, we characterised the eicosanoid pattern score (EPS). A novel technique based on binomial errors on lipid mediator partitioning enabled calibration of in vivo biochemical parameters in molecular units. We found that eicosanoid production rates fluctuate over a time scale of about twenty minutes, while intrinsic noise decays rapidly. Thus, biochemical eicosanoid parameters, noise, and slowly varying cellular states together determine the effective FET. These results can form a basis for quantitative modelling of natural eicosanoid circuits in diagnosis of eicosanoid related diseases and design of synthetic ones for the prediction other diseases.

6

Reactivity of monoclonal antibodies OK-CLL [anti-CD5] with peripheral blood cells of patients with B cell lymphoproliferative disorders

84%

Milosevic D., Marinkovic M., Jelic S., Colovic M., Spuzic I.

Archivum Immunologiae et Therapiae Experimentalis

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1995

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tom 43

|

nr 1

7

Interferon production by peripheral blood cells of patients with alcoholic liver disease [ALD]

84%

Daniluk J., Kandefer-Szerszen M.

Archivum Immunologiae et Therapiae Experimentalis

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1994

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tom 42

|

nr 3

8

Hematopoiesis in snakes [Ophidia]

84%

Dabrowski Z., Tabarowski Z., Sano-Martins I. S., Spadacci-Morena D. D., Witkowska-Pelc E., Krzysztofowicz E., Spodaryk K.

Folia Histochemica et Cytobiologica

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2002

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tom 40

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nr 2

9

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Human cytomegalovirus infection as a lifelong health problem

67%

Figlerowicz M., Modlinska-Cwalinska A., Mania A., Mazur-Melewska K., Kemnitz P., Jonczyk-Potoczna K., Sluzewski W.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2011

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tom 92

|

nr 4

10

Deviations in peripheral blood cell populations are associated with the stage of primary biliary cholangitis and presence of itching

67%

Cuchoz-Lach H., Grywalska E., Michalak A., Kowalik A., Mielnik M., Rolinski J.

Archivum Immunologiae et Therapiae Experimentalis

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2018

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tom 66

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nr 6

Ograniczanie wyników

Spectral properties of phthalocyanines incorporated into resting and stimulated human peripheral blood cells

Selected peripheral blood cell parameters in twelve inbred strains of laboratory mice

Effect of granulocyte-macrophage colony-stimulating growth factor on interferon and tumor necrosis factor production in whole blood cell cultures of patients with acute myelogenous leukemia

Gene expression profiling in peripheral blood nuclear cells in patients with refractory ischaemic end-stage heart failure

Functional eicosanoid test and typing [FET] of peripheral blood cells in eicosanoid related diseases

Reactivity of monoclonal antibodies OK-CLL [anti-CD5] with peripheral blood cells of patients with B cell lymphoproliferative disorders

Interferon production by peripheral blood cells of patients with alcoholic liver disease [ALD]

Hematopoiesis in snakes [Ophidia]

Human cytomegalovirus infection as a lifelong health problem

Deviations in peripheral blood cell populations are associated with the stage of primary biliary cholangitis and presence of itching