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The aim of the study was to determine plasma levels of vitamin C and reduced glutathione (GSH) in sows between the day 14 prepartum and day 14 postpartum. The study involved twenty-four sows of three breeds - Polish Large White (PLW), Polish Landrace (PL) and PLWxPL aged 1-3 years. All the animals were from one closed-cycle production farm. The mean vitamin C level on days 13-14 prepartum reached 0.49 ±0.19 mmol/g of protein and decreased significantly (P<05) at 24-48 h postpartum to 0.33 ±0.19 mmol/g of protein. On days 6-7 and 13-14 postpartum, the vitamin C level further decreased to 0.17 ±0.006 and 0.15 ±0.007 mmol/g of protein, respectively. The mean GSH level on days 13-14 before delivery was 0.071 ±0.009 mmol/g of protein and decreased significantly (P0.05) at 24-48 h before delivery to 0.062 ±0.018 mmol/g of protein. In this period, the mean GSH level was similar to that observed during the first 24-48 h postpartum. On day 6-7 after delivery, the level of GSH reached the values observed on days 13-14 and 6-7 prepartum. On days 13-14 postpartum, the level of GSH was found to be 0.115 ±0.029 mmol/g of protein and was significantly higher (P<0.001) compared to that on days 13-14 prepartum. The findings suggest that porcine levels of vitamin C and glutathione decrease during the periparturient period, which may lead to a decreased antioxidant defence system and an imbalance in redox homeostasis.
The aim of the present study was the description of the dynamics in erythrocyte GSH-Px activity and serum selenium content changes in sows during 14 days before and 14 days after parturition. The experiment was performed on 36 gilts of 1-3 years old. All animals came from the same farm with a closed production system and were fed the same diet of unknown selenium content. Blood was collected 8 times: 13-14 days before parturition, 6-7 days before parturition, 48-72 hours before parturition, and 12-24 hours before parturition, as well as 12-24 hours after parturition, 48-72 hours after parturition, 6-7 days after parturition and 13-14 days after parturition, always after feeding. Blood was centrifuged to obtain serum and erythrocytes were hemolysed. Erythrocyte GSH-Px activity was determined by the Paglia and Valentine method and expressed in U per g Hb. Serum selenium content was determined by the atomic absorption method. GSH-Px activity 14 days before parturition reached 13.38 U/g Hb and significantly decreased around the 7th day before parturition to 11.26 U/g Hb. 72 hours before parturition the activity of the enzyme increased to 16.85 U/g Hb. Between the 72nd hour before and the 14lh day after parturition GSH-Px activity remained constant. Serum selenium content did not differ significantly in the examined gilts and ranged between 88.9 ug/L and 121.87 ug/L. 24 hours before and 24 hours after parturition the selenium content reached 96.55 ug/L and 88.91 ug/L respectively.
Experiment was conducted with 36 dairy cows (in II-VI lactation), divided as analogues to three groups per 12 weeks (3 weeks before calving to 0 day – day of calving and second period first 70 days of lactation): group I – without glycerin supplement, group II – 300 ml·head-1·day-1 and III – 500 ml·head-1·day-1 supplemented to daily feed ration. All cows were fed two kinds of TMR, according to DLG requirements for each physiological phases: for dry period and for the first 100 days of lactation. The body condition, feed intake, milk production, milk chemical composition and main blood metabolites as glucose, BHB, NEFA were analyzed in experimental period. Total milk production or mean daily milk yield in group II and III was higher in 10 weeks of lactation in comparison to control group about 14.6 and 12.5% respectively. With increase dose of glycerin the protein level in milk was increased (P<0.05). In group III in 3rd week of lactation the concentration of BHB in blood serum was higher but glucose lower in comparison to other groups. The concentration of NEFA in blood serum in group III in 1st and 3rd weeks of lactation and in group II in 3rd week after calving was lower in comparison to control (I) group. The feed intake (in DM) of TMR was better in both groups with supplemented glycerin in comparison to control, especially between 4. and 9. week of lactation (P<0.05). At 70 days in cows fed TMR with glycerin better body score condition index was observed in comparison to control (P<0.05).
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