Wyniki wyszukiwania - AGRO

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Micropropagation of Harpagophytum procumbens [Burch.] DC. ex Meisn.: the effect of cytokinins on shoot multiplication

100%

Grabkowska R., Wysokinska H.

Herba Polonica

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2009

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tom 55

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nr 3

244-250

The procedure of in vitro propagation of Harpagophytum procumbens using shoot tips was developed. Shoot tips were cultured on Schenk and Hildebrandt (SH) agar medium supplemented with 0.57 µM indole-3-acetic acid (IAA) in combination with various cytokinins: 6-benzylaminopurine (BAP), thidiazurone (TDZ), kinetin or zeatin at four concentrations (2, 4, 6 or 8 µM). The best shoot multiplication rate (11.2 shoots/explant for 5 weeks) was achieved in the presence of 6 µM TDZ. The shoots were small and their elongation on SH medium supplemented with gibberellic acid (GA3 ) was necessary. Shoots of H. procumbens were rooted on full-strength or half-strength Murashige and Skoog (MS) agar medium either with or without auxin. Plantlets were transferred into pots and maintained in the greenhouse. After 1 month, the overall survival of plants was 83% but it decreased to 27% after 6 months.

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Rhaponticum carthamoides regeneration through direct and indirect organogenesis, molecular profiles and secondary metabolite production

100%

Skala E., Grabkowska R., Sitarek P., Kuzma L., Wysokinska H.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

3

Effects of site conditions on sexual dimorphism and sex ratio in lowland populations of Aruncus sylvestris Kostel. (Rosaceae)

84%

Marciniuk J., Marciniuk P., Rymuza K.

Polish Journal of Ecology

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2010

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tom 58

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nr 2

Studies on sexual dimorphism in reference to vegetative features and on the sex ratio in different environmental conditions were carried out in lowland populations of dioecious species Aruncus sylvestris. It was shown that female individuals of Aruncus sylvestris produced higher flowering shoots, longer inflorescences, more leaves and longer and broader leaves than the male ones. All listed differences were statistically significant. In populations of Aruncus sylvestris growing in optimum ecological conditions (fertile and wet soil, half-light) the sex ratio M/F is close to 1:1. Changes of ecological conditions along the gradient of fertility, habitat moisture (estimated by the types of phytocoenoses) and intensity of solar radiation caused marked drift of sex structure towards the male domination. Light intensity was the crucial factor for the sex ratio in studied populations. Populations growing in half-light showed sex ratio M/F ≈ 1:1 irrespective of habitat. Those growing in shade and in full light showed significant shift to male dominance. The largest deviation from 1:1 sex ratio (M/F ≈ 1.0:0.2) was observed in extremely unfavorable habitat conditions (full sun irradiance, dry soil, strong competition from Elymus repens (L.) Gould). Obtained results demonstrated that female individuals of Aruncus sylvestris had markedly higher habitat requirements compared with the male ones.

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Response of three ornamental perennials to drought stress

84%

Chylinski K., Lukaszewska A.

Annals of Warsaw University of Life Sciences - SGGW. Horticulture and Landscape Architecture

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2010

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tom 31

5

Biomass accumulation and diterpenoids biosynthesis in Salvia austriaca transformed roots grown in a bioreactor

84%

Kuzma L., Klos K., Wysokinska H.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

6

The effect of corm planting date and depth on Acidanthera bicolor Hochst. cormel yield

84%

Laskowska H., Kocira A.

Folia Horticulturae

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2004

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tom 16

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nr 1

147-152

7

Micropropagation of Carlina acaulis L.

84%

Trejgell A., Bednarek M., Tretyn A.

Acta Biologica Cracoviensia. Series Botanica

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2009

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tom 51

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nr 1

97-103

An efficient shoot propagation system for Carlina acaulis was developed in this study. The experimental material consisted of shoot tips and fragments of hypocotyls excised from 10-day-old seedlings. The explants were transferred to proliferation medium supplemented with different types of cytokinins: 6-benzylaminopurine (BA, 4.4 or 13.3 µM), kinetin (Kn, 4.7 or 13.9 µM) and zeatin (Zea, 4.6 or 13.7 µM) in combination with naphthaleneacetic acid (0.54 µM NAA). The morphogenetic response was best in culture on medium supplemented with 13.3 µM BA, and shoot organogenesis frequency was highest for shoot tips (100%). On average, 7.5 shoots were induced per explant of the initial material, and the multiplication rate in five subsequent subcultures was 6.1. Shoot length was lower in culture with BA in the medium than with Kn or Zea. Plantlets rooted with 60% frequency in vitro on full-strength MS medium and with 55.3% frequency ex vitro. Reduction of the mineral salt concentration (1/2MS) stimulated rhizogenesis. Addition of auxins stimulated both the frequency and number of roots per shoot, but only in combination with full-strength MS medium. Regenerated plants were able to flower and gave viable seeds.

8

A protocol for synthetic seeds from Salvia officinalis L. shoot tips

84%

Grzegorczyk I., Wysokinska H.

Acta Biologica Cracoviensia. Series Botanica

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2011

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tom 53

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nr 1

Shoot tips excised from shoot culture of Salvia officinalis were encapsulated in 2% or 3% (w/v) sodium alginate and exposed to 50 mM calcium chloride for complexation. Immediately or after 6, 12 or 24 weeks of storage at 4°C, the synthetic seeds were cultured for 6 weeks on half-strength MS medium supplemented with indole-3- acetic acid (IAA) (0.1 mg/l) and solidified with 0.7% agar. The frequency of shoot and root emergence from encapsulated shoot tips was affected by the concentrations of sodium alginate and additives in the gel matrix (sucrose, gibberellic acid, MS nutrient medium) as well as duration of storage. The frequency of shoot and root induction of non-stored synthetic seeds was highest with shoot tips encapsulated with 2% sodium alginate containing 1.5% sucrose and 0.5 mg/l gibberellic acid (GA3). Shoot tips maintained their viability and ability to develop shoots even after 24 weeks of storage when they were encapsulated in 3% alginate with 1/3 MS medium, sucrose (1.5%) and GA3 (0.25 mg/l). Root formation tended to decrease with storage time. Overall, 90% of the plantlets derived from stored and non-stored synthetic seeds survived in the greenhouse and grew to phenotypically normal plants. This procedure can enable the use of synthetic seed technology for germplasm conservation of S. officinalis, a plant species of high medical and commercial value.

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Variation in stage structure and fitness traits between road verge and meadow populations of Colchicum autumnale [Liliaceae]: effects of habitat quality

84%

Mroz L.

Acta Societatis Botanicorum Poloniae

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2006

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tom 75

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nr 1

69-78

The frequency distribution and density of three life stages as well as fitness components of the perennial plant Colchicum autumnale growing in the unmanaged road verges and in the extensive mown and grazed meadows in the Sudeten Mts. were studied. Furthermore, investigated were the effects of population size and plant size (measured as number of flowers) on reproductive success and explored if variation in reproductive and vegetative traits of adults could be associated with soil characteristics. The t-test indicated that proportions of subadults and reproductive adults were significantly lower in verge than in meadow populations, and of vegetative adults significantly higher. The plant density of reproductive adults and the reproductive adults to all adults ratio were significantly lower in verge populations compared to meadow populations. Although habitat type accounted for significant variation in stage structure, no significant difference was found between vegetative and reproductive traits in adult plants, except for the number of flowers. In verge populations the number of flowers was significantly lower as compared to meadow populations. The traits related to reproduction were not significantly influenced by population size. However, the proportion of flowers setting fruit decreased significantly with increasing number of flowers. The stepwise multiple regression revealed significant relationships between soil characteristics and number of fruits per plant, fruit set, number seeds per plant and number of leaves in vegetative adults. The results suggest that the creation of the low and relatively open vegetation cover could increase the chance of persistence of C. autumnale living in verge habitats by promoting of seed germination, seedling establishment and flowering, and they also show that the reproductive success and vegetative components of fitness are most likely influenced by habitat quality.

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Allometric partitioning theory versus optimal partitioning theory: the adjustment of biomass allocation and internal C-N balance to shading and nitrogen addition in Fritillaria unibracteata (Liliaceae)

84%

Guo H., Xu B., Wu Y., Shi F., Wu C., Wu N.

Polish Journal of Ecology

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2016

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tom 64

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nr 2

Fritillaria unibracteata is a classic perennial alpine herb. In this study, we examined it's responses to shading (SH) and nitrogen addition (NA), as well as its correlation with internal C-N balance to detect how it adjusted to the changes of habitat conditions. Randomized block experiment was carried out in the field in Chuanbeimu Research Station in Songpan County, Sichuan Province, China (32°09′54″N, 103°38′36″E, altitude 3300 m a.s.l.). Two growing seasons after NA and SH, Fritillaria unibracteata's total plant biomass decreased significantly, with the proportion of biomass allocated to aboveground significantly increased. In addition, in this study, under both SH and NA treatments, Fritillaria unibracteata increased its biomass allocation to above-ground, which consisted with optimal partitioning theory. Moreover, Fritillaria unibracteata's biomass allocation was significantly correlated with its internal C-N status, regardless of nitrogen and light condition. We conclude that Fritillaria unibracteata optimizes its biomass allocation between root and shoot by adjusting its internal C-N balance, which would not be changed by the specialized resource storage organ-bulb.

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In vitro root cultures of Lychnis flos-cuculi L. as a potential source of triterpenoid saponins and ecdysteroids

84%

Malinski M., Sienicka J., Thiem B.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

12

Impact of herbivorous dock leaf beetle [Gastroidea viridula Deg.] releasing on the development of mossy sorrel [Rumex confertus Willd.]

84%

Piesik D., Lamparski R.

Electronic Journal of Polish Agricultural Universities. Series Biology

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2006

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tom 09

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nr 4

13

Possibilities of using perennial energy crops in reclamation of degraded land

84%

Radwanska K., Zadrozniak B., Mystkowska I., Baranowska A.

Economic and Regional Studies

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2016

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tom 09

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nr 4

Subject and purpose of work: The work is of a review character. The present paper aims to introduce basic definitions related to degradation and reclamation of the areas with lower use value, and also to present the most important species of perennial energy crops, which can be possibly used in the reclamation of degraded soils. Materials and methods: This work is based on collected information concerning soil degradation, perennial energy crops and their use in the reclamation. Results and conclusions: Characteristics of recommended species of perennial energy crops to a reclamation of degraded soils: willow, Virginia fanpetals, the Jerusalem artichoke, giant miscanthus, amur silver-grass, prairie cordgrass, rosa rugose, black locust, ashleaf maple has been presented. The analysis of the soil degradation in Lublin Voivodeship has been based on the professional literature. On that basis, the plants which would be the best in the reclamation of this area have been chosen. This work also takes into account the estimated costs of establishing plantations of these plants

14

Large-scale vs. small-scale factors affecting flowering patterns in Senecio macrophyllus M.BIEB., a long-lived perennial

84%

Czarnecka B.

Acta Agrobotanica

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2006

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tom 59

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nr 1

The aim of the study was to establish which of environmental factors affect flowering patterns in a long-lived herbaceous plant Senecio macrophyllus. The study was conducted in the population of Biała Góra (White Mountain) near Tomaszów Lubelski in the years 1989-2004. The results allow to conclude that in the reproductive patterns of the analysed population small-scale environmental factors (soil moisture and trophism, light intensity) are more important than large-scale climatic factors (temperature, precipitation), which to a comparable extent affect the plants in the whole population area.

15

Algorithm for determination of relative age of above-ground parts of Rhododendron hirsutum and Rhododendron ferrugineum [Ericaceae]

84%

Tumidajowicz D., Dawidowicz A. L.

Polish Botanical Studies

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1993

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tom 05

16

First report of twig blight of ban tulsi (Croton bonplandianus Bail.) caused by Choanephora cucurbitarum (Berk.and Ravenel) Thaxt. in India

67%

Das S., Dutta S., Ray S. K.

Journal of Plant Protection Research

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2017

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tom 57

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nr 4

In August 2015, twig blight disease of ban tulsi (Croton bonplandianus Bail.) caused by Choanephora cucurbitarum (Berk. & Ravenel) Thaxt. was observed for the first time, in the Gangetic alluvial region of West Bengal, India. A severe disease incidence (40–50%) showed twig blight symptoms starting with shoot apical meristem (SAM), leaf, and blossom blight symptoms. Typical symptoms were characterized by over-projecting black pin head like emerging sporangiola which formed mycelial cushion on the infected surface. The present report describes the identification of the causal pathogen as C. cucurbitarum based on its morphology and the internal transcribed spacer of its ribosomal DNA (rDNA – ITS) sequences with 100% identity of NCBI-GenBank published Choanephora database. Optimum temperatures, 28–30°C, coupled with high relative humidity (80–90%) during the monsoon season enhances the disease’s progress. To the best of our knowledge this is the first report of twig blight of C. bonplandianus, caused by C. cucurbitarum, in India as well as globally.

17

Cytological studies on Microdochium nivale infection in Lolium perenne (L.) plants

67%

Dubas E., Marzec K., Plazek A.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2010

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tom 545

Winter hardiness of plants is limited mainly due to sensibility to Iow temperature and winter fungus damages. Snow mould is a disease that affects many species of winter cereals, turf and forage grasses. In Poland it is caused mainly by the common and economically important fungal pathogen Microdochium nivale causing pink snow mould, destructive disease of seedlings, stem bases and ears. The aim of the study was to investigate the dynamie of Microdochium nivale infection and cytological changes in cold-hardened Lolium perenne Danish cv. Darius. Plants were inoculated with mycelium. The studied plants demonstrated fast defence response to fungus attack, accumulating phenolics already one day after inoculation, however no infecting hyphae within leaf tissue at this time were detected. The first infection hyphae in plants were observed two days after inoculation. Microscopy observations revealed sheaths of the youngest leaves located in the crowns as the main infection way. These observations showed that Microdochium nivale hyphae uptake nutrients from the host by haustoria damaging the protoplasts of mesophyll cells causing their strong plasmolysis. This type of feeding is characteristic for biotrophic and hemibiotrophic parasites. Our observation proved that Microdochium nivale is a hemibiotrophic pathogen and inoculation with its mycelium is very effective and fast and causes serious damages of Lolium perenne cells.

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Assessment of farmers’ potential decisions on setting up perennial plantations of energy plants

67%

Osiak J., Skudlarski J., Izdebski W.

Teka Komisji Motoryzacji i Energetyki Rolnictwa

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2009

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tom 09

19

Gatunki rodzaju Phytophthora jako czynniki ograniczajace plon i jakosc roslin ozdobnych

67%

Orlikowski L. B.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2006

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tom 510

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nr 2

407-411

Phytophthora cinnamomi, P. citrophthora i P. cryptogea izolowano z około 3-80% zamierających roślin cyprysika Lawsona, lawendy, rojnika, skalnicy i ubiorka. W naturalnych warunkach uprawy pojemnikowej cyprysika i bylin fytoftoroza pojawiała się na roślinach już po 4 tygodniach uprawy, a w okresie 4-6 miesięcy uprawy straty spowodowane zamieraniem roślin wahały się od kilku do 71%. Źródłem Phytophthora spp. mogły być sadzonki oraz woda.

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Kolorowe jak pisanki

67%

Narkiewicz M.

Kwietnik

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2006

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nr 04

18-19

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