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1

Biodegradation of phenol in batch culture by pure and mixed strains of Paenibacillus sp. and Bacillus cereus

100%
Singh S., Singh B. B., Chandra R.
Polish Journal of Microbiology
|
2009
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tom 58
|
nr 4
319-325
The bacterial strains, Paenibacillus sp. (AY952466) and Bacillus cereus (DQ002384), have proven capacity to degrade lignin and pentachlorophenol. In the present study, both strains were screened at different concentrations of phenol on mineral salt agar medium in the presence of glucose. At optimized condition (pH 7.5 ± 0.2,37 ± 1°C, 120 rpm, 1% glucose w/v), it was observed that both Paenibacillus sp., B. cereus and its mixed culture degraded phenol (500 mg/l) up to 53.86%, 91.63% and 67.76% within 168 h of incubation, respectively. Phenol degradation was routinely monitored spectrophotometrically and further confirmed by HPLC. Catechol and 2-hydroxy muconic semialdehyde were identified as intermediate products from degraded samples using GC-MS. It was also observed that, in the absence of glucose, bacterial strains were unable to utilize phenol indicating the phenomenon of co-metabolism.
2

Polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and pentachlorophenol (PCP) in bottom sediments of the Port of Gdansk

100%
Lewandowski K., Witt M., Kobusinska M., Niemirycz E.
Oceanological and Hydrobiological Studies
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2014
|
tom 43
|
nr 3
3

Factors determining the accumulation of pentachlorophenol − a precursor of dioxins in bottom sediments of the Gulf of Gdansk (Baltic Sea)

100%
Kobusinska M., Skauradszun M., Niemirycz E.
Oceanological and Hydrobiological Studies
|
2014
|
tom 43
|
nr 2
4

Immunoassays and environmental studies

80%
Plaza G., Ulfig K., Tien A. J.
Polish Journal of Environmental Studies
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2000
|
tom 09
|
nr 4
Immunoassays (immunochemical methods - IMAs) are now being seen as useful analytical tools, and supplements to conventional analytical methods: gas chromatography - GC and high performance liquid chromatography - HPLC. Immunochemical methods provide rapid, sensitive, and cost effective analyses for a variety of environmental contaminants. The development of the methods is multidisciplinary. IMAs combine principles of immunology and chemistry into tests that are used by scientists in practically every disciplines, including fields as diverse as molecular biology and environmental science. All immunoassays rely on the interaction between an antibody as analytical reagents and a target analytes (antigen). Environmental immunoassays have been developed and evaluated for analytes including major classes of pesticides, organic compounds as polychlorinated biphenyls (PCBs), polyaromatic hydrocarbons (PAHs), pentachlorophenols (PCPs), BTEX (benzene, toluene, ethylbenzene, and xylene), dioxins and furans, and some inorganics, for example cadmium, lead, mercury, and microbial toxins. This paper provides an overview on the possibilites of immunoassays as a detection method for environmental contaminants. The principles and the history of the immunoassay methodology are reviewed.
5

Genotoxicity of pentachlorophenol revealed by Allium chromosome aberration assay

80%
Pavlica M., Vasilevska J., Papes D.
Acta Biologica Cracoviensia. Series Botanica
|
1998
|
tom 40
Pentachlorophenol (PCP) is known to be a very effective biocide, used very widely a few years ago. Eventually its use was stopped in many countries when it was shown that PCP is highly toxic, especially to water organisms, and to some land animals and plants. However, PCP is still present in the environment, mostly as a metabolic product of some pesticides. Roots of shallot (Allium ascalonicum auct.) were exposed to different concentrations of PCP: 0.001, 0.01, 0.1 and 1 mg/dm3 for 24 h. The tested solutions were adjusted to pH 3.0, 6.0 or 8.0. Root tip cells were analyzed either after 24 h of treatment or after treatment and 48 h recovery in water. Genotoxic effects of PCP in root tip cells were noted, the extent of which depended on the PCP concentration and pH. Chromosome and mitotic aberrations were found, including the significant occurrence of micronuclei, chromosome bridges and multipolar cells.
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