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An extensive survey of downy mildew (Sclerospora graminicola) incidence on pearl millet (Pennisetumglaucum) grown in farmers’ fields was undertaken in 2002 in pearl millet-growing areas of Nigeria – the Sahel, Sudan Savanna, northern Guinea Savanna and southern Guinea Savanna agroecological zones. Downy mildew incidence was assessed on two types of pearl millet “gero” and “maiwa” and in 32 locations in these zones. Incidence in “gero” millet was the highest in Sudan Savanna and the lowest in the Sahel zone. Cropping pattern and seed rate affected incidence of the disease which was highest in the fields with pure stand than mixed stand cropped with legumes..
The obligate oomycete Sclerospora graminicola (Sacc.) Schroet, is the incitant of downy mildew disease, which is the main constraint in pearl millet production worldwide. Different elicitors from Trichoderma hamatum UOM 13, e.g. mycelial extract and cell wall glucans, were assessed for their resistance elicitation efficiency and the possible underlying mechanisms. Both mycelial extract and cell wall glucans of T. hamatum UOM 13 positively influenced seed quality parameters of pearl millet, significantly enhanced seed germination and seedling vigor in comparison to the untreated control. Seed priming with cell wall glucan elicitors of T. hamatum UOM 13 suppressed downy mildew on susceptible pearl millet seedlings under greenhouse conditions by induction of systemic host resistance. Of the different elicitor delivery methods tested, transplant root dip was more effective than seed treatment and foliar spray. A combination of transplant root dip + seed treatment + foliar spray was significantly more effective than the single delivery methods. The induced resistance corresponded to up regulation of genes of important defense proteins upon pathogen inoculation. Transcripts of genes of defense enzymes glucanase, phenylalanine ammonia lyase, peroxidase and polyphenoloxidase were significantly increased due to the T. hamatum UOM elicitor effect. Expression of hydroxyproline-rich glycoprotein genes, known to play an important role in cell wall cross-linking, were also up regulated in response to T. hamatum UOM cell wall glucan treatment. This study emphasizes the role of T. hamatum UOM as a potential elicitor of downy mildew resistance in pearl millet and presents novel insights into the involvement of important defense proteins mediating such as resistance trigger.
Three separate field experiments were conducted in 2001, 2002 and 2003 to determine the efficacy of combining sowing date, seed treatment with metalaxyl and use of host plant resistance for the control of pearl millet downy mildew. Early sowing gave lower disease incidence and higher grain yield than late sowing. The disease was controlled when metalaxyl treated seeds were sown early. The highest disease incidence and the lowest grain yields were obtained when untreated seeds were sown late. Use of resistant pearl millet cultivar along with seed treatment using metalaxyl at the dose of 2 g a.i./kg seeds greatly reduced disease incidence and increased grain yield in comparison with the seed treatment of susceptible cultivar.
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