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Aspergillus parasiticus is one of the most common fungi which contaminates peanuts by destroying peanut shells before they are harvested and the fungus produces aflatoxins. The aim of this study was to evaluate the antifungal activities of seventeen essential oils on the growth of the aflatoxigenic form of A. parasiticus in contaminated peanuts from commercial outlets in Georgia. The agar dilution method was used to test the antifungal activity of essential oils against this form of A. parasiticus at various concentrations: 500; 1,000; 1,500; 2,000; 2,500 ppm. Among the seventeen essential oils tested, the antifungal effect of cinnamon, lemongrass, clove and thyme resulted in complete inhibition of mycelial growth. Cinnamon oil inhibited mycelial growth at ≥ 1,000 ppm, lemongrass and clove oils at ≥ 1,500 ppm and thyme at 2,500 ppm. However, cedar wood, citronella, cumin and peppermint oils showed partial inhibition of mycelial growth. Eucalyptus oil, on the other hand, had less antifungal properties against growth of A. parasiticus, irrespective of its concentration. Our results indicate that the aflatoxigenic form of A. parasiticus is sensitive to selected essential oils, especially cinnamon. These findings clearly indicate that essential oils may find a practical application in controlling the growth of A. parasiticus in stored peanuts.
Mutation breeding has been widely used for the improvement of plant characters in various crops. It is a powerful and effective tool in the hands of plant breeders. In any mutation breeding program, selection of an effective and efficient mutagen is very essential to produce high frequency of desirable mutation. Groundnut (Arachis hypogia) var. VRI-2. was treated with different concentration of physical and chemical mutagen namely gamma rays 10, 20, 30, 40, 50 and 60 KR and Ethyl methane sulphonate (EMS). For inducing mutation various concentration of EMS such as 0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 % for six hours were applied to 200 seed sample of each concentration and one respective control. The LD50 value was observed in 50% of gamma rays and 0.5 % of EMS. The morphological and yield characters were significantly reduced seed germination, seedling survival, days to first flower, plant height, number of leaves per plant, number of grains per plant, grain length and breath, 100 grains weight, grain yield per plant, fresh and dry weight per plant. The increasing doses/concentration of gamma rays and EMS decreased in phenotypic and yield characters in M1generation. The mutagenized populations showed significantly higher variability in the M2 generation. Mutant lines showing higher yield per plant than the respective parents and checks were isolated in M2 and subsequent generation were significantly more pod yield and yield components than the untreated plants.
The aim of the study was to evaluate the quality of peanuts available on the Olsztyn market. The quality of researched peanuts was evaluated by determining degree hydrolysis and oxidation of oils and composition of fatty acids. Out of the nine peanut samples under examination, the content of primary and secondary products of oxidation was very high in eight of them, which could indicate their unsuitability for consumption. A high content of secondary products of oxidation proves an advanced degree of peanut deterioration, which, considering the good use-by date for seven out of nine samples, leads to the suspicion that the purchased peanuts were old and damaged, as indicated by the high values of lipid oxidation indices. While purchasing peanuts for consumption, the customer is not able to learn their nutritional value, since it is provided laconically and very sparingly. The use-by date printed for peanuts turns out to be false since it is not accompanied by their quality. We believe that consumers are entitled to know the required information about the products, e.g. the country of origin of the raw material, its freshness status, production method (whether the peanuts were roasted in siliques and later shelled and packed), the parameters of roasting and frying, the type of oil used, etc.
The field experiment was conducted with four tillage and three nutrient management practices at Agronomy field unit, University of Agricultural Sciences, GKVK, Bangalore, Karnataka, India. The results of the study indicated that, higher groundnut pod yield (1307 and 1282 kg ha-1) and haulm yield (2733 and 2889 kg ha-1) was recorded with mechanical tillage + two intercultivations and integrated application of 50% organics and 50% inorganics, respectively as compared to rest of the treatments. Further, the same treatments noticed higher availability of N, P2O5 and K2O in soil after harvest of groundnut, but greater improvement in soil organic carbon content was observed with conventional tillage + one intercultivation (0.62%) as compared to rest of the tillage practices. The maximum net returns and benefit-cost ratio was recorded with mechanical tillage + two intercultivations (Rs. 11,287 ha-1 and 1.75, respectively) and integrated application of 50% organics + 50% inorganics (Rs. 10,462 ha-1 and 1.69, respectively). However, interaction effect of tillage and nutrient management practices were not influenced significantly on soil fertility and productivity of groundnut under rainfed farming system.
The field experiment was conducted in split design with three replicates in Arunagirimangalam village, Thiruvanamalai District to study the effect of groundnut haulm compost of single and combined inoculation of FYM, different biofertilizers (Azotobacter, Azospirillum, Phosphobacteria, Rhizobium) on growth, photosynthetic pigments, biochemical properties and yield of Arachis hypogeae L. The results of this experiment revealed that groundnut haulm with combined inoculation of biofertilizers significantly increased growth, photosynthetic pigments, biochemical and yield parameters of the test crop. Among various combinations, single inoculums of groundnut haulm performed better than control while groundnut haulm with combined inoculation of Rhizobium found better over single inoculation treatments. In general, it could be concluded that groundnut haulm compost and mixed with Rhizobium may be a good tool to improve the crop yield productivity and quantity and soil health.
The antifungal activity of the ethanolic and aqueous fruit extracts of Zingiber officinale, Tetrapleura tetraptera, Garcinia kola and Cola nitida on the green rot fungus (Penicillium sp.) isolated from infected groundnut (Arachis hypogaea) seeds in storage were investigated in vitro. Various concentrations of the aqueous and ethanolic fruit extract ranging from 5g/100ml, 10g/100ml, and 15g/100ml were separately added to Potato Dextrose Agar (PDA) media. The fungus was inoculated into the media and incubated for seven days. Results of the in vitro studies showed that the ethanolic extracts had a significant inhibitory effect (p<0.05) on the radial growth of Penicillium sp. at all levels of concentration tested. Complete inhibition of the fungus was at 15g/100ml of ethanolic extracts of Garcinia kola and Tetrapleura tetraptera. The aqueous extracts showed less inhibitory effect as compared to the ethanolic extracts. The effective inhibition of ethanolic extracts as compared to the aqueous extracts may be attributed to the efficiency of the extraction solvent on the phytochemical content of the plant extracts. The application of botanical extracts for disease management could be less expensive, easily available, non-polluting and ecofriendly. The ethanolic fruit extracts of Garcinia kola and Tetrapleura tetraptera at the higher concentrations can be produced in large quantities and used as spray in controlling the green rot fungus of Arachis hypogaea in storage.
Celem podjętych badań była kompleksowa analiza zmian jakości przechowywanych orzeszków ziemnych, pakowanych w nowoczesne laminaty w modyfikowanej atmosferze (MAP). Analizowano zmiany zawartości O2 w opakowaniu oraz zmiany cech sensorycznych i fizykochemicznych produktów w zależności od rodzaju opakowania i okresu przechowywania. Stwierdzono, że zmiany zawartości tlenu w stosowanych opakowaniach charakteryzowały się zbliżonym przebiegiem, niezależnie od rodzaju ich producenta. W czasie przechowywania zaobserwowano zmiany jakości tłuszczu w produkcie, czego przejawem były głównie wzrost liczby nadtlenkowej i pogorszenie walorów smakowo-zapachowych.
W pracy przedstawiono możliwość zastosowania testu immunoreaktywnego RIDASCREEN® Peanut firmy R-Biopharm do oznaczania zawartości podstawowego alergenu orzechów arachidowych, Ara h 1 w surowcach roślinnych i wyrobach cukierniczych. Ogółem analizie metodą sandwich ELISA poddano 19 próbek (9 stanowiły surowce roślinne zaś 10 czekolady, batoniki i kremy do smarowania pieczywa). Obecność Ara h 1 stwierdzono we wszystkich produktach cukierniczych, również w tych, w których składzie producent nie deklarował dodatku orzechów arachidowych. Wśród wybranych surowców roślinnych trzy z nich (migdały - Amygdalus communis, soja - Glycine max, owies - Avena) zareagowały z przeciwciałem skierowanym do Ara h 1.
The aim of the study was to analyse the potential pea-peanut cross-reactivity using the mice BALB/c as a biological in vivo model in the research on immune response to peanut proteins (PnE). BALB/c mice were three-fold sensitised (on days 1, 7, and 21) by oral or intraperitoneal (IP) administration of PnE in 0.5 mg or 1 mg dose, with or without adjuvant – aluminum hydroxide gel (Alum). Serum immunoglobulins (IgE, IgG, IgG1 and IgG2a) and level of cytokines (IL-4, IL-10, IFN- γ), secreted by the isolated lymphocytes were examined. The highest increase in total IgE and peanut-specific IgG1 was noted in the group sensitised by IP administration of PnE in the presence of Alum. Lymphocytes from peanut-sensitised (with and without Alum) mice showed a significantly high level of IL-4 and this cytokine was secreted to a much higher extent as compared to IFN-γ. Stimulation of a culture of lymphocytes with pea proteins resulted in high IFN-γ secretion. A weak reaction of peanut-specific IgG1 present in mice serum with pea globulins (vicilin – PV and legumin – PL) can suggest that the cross-reactivity between peanut and pea proteins results from the presence of proteins other than 7S and 11S globulins. Due to the demonstrated low cross-reactivity between peanut proteins and pea globulins, the possibility of applying pea proteins in peanut-allergy immunotherapy may be suggested.
Based on a three-year field experiment under controlled condition in Ji’nan, China, the effects of peanut growth on the variation in the abundance and community structure of ammonia oxidizing bacteria (AOB) and archaea (AOA) before and after peanut growth were investigated through quantitative PCR and cluster analysis of terminal-restriction fragment length polymorphism. Our results show that the community composition of AOA and AOB was greatly affected by the peanut growth leading to the decreased abundance of AOA and increased abundance of AOB. Furthermore, AOA and AOB community structures varied before and after peanut growth. Phylogenetic analysis indicated that all AOA and AOB community sequences were clustered into the uncultured group. Altogether, the results suggested that the abundance of AOA and AOB in soil and their community compositions can be greatly affected by the peanut growth.
Celem pracy było zbudowanie za pomocą skanera 3D modeli numerycznych owoców orzecha włoskiego bez okrywy zewnętrznej, orzechy podziemnej i leszczyny pospolitej, określenie cech geometrycznych owoców na podstawie tych modeli i porównanie z wynikami uzyskanymi za pomocą cyfrowej analizy obrazu, pomiarów suwmiarką oraz piknometrem cieczowym. Średnia masa owocu orzecha włoskiego jest większa od średniej masy owocu orzechy podziemnej o około 84% i od średniej masy owocu leszczyny pospolitej o około 83%. Na podstawie wyników uzyskanych po skanowaniu skanerem 3D wynika, że średnie pole powierzchni owocu orzecha włoskiego jest o 61% większe od średniego pola powierzchni owocu orzechy podziemnej i o 72% większe od średniego pola powierzchni owocu leszczyny pospolitej. Średnia objętość owocu orzecha włoskiego jest o 78% większa od średniej objętości owocu orzechy podziemnej i o 84% większa od średniej objętości owocu leszczyny pospolitej. Modele 3D owoców badanych gatunków uzyskane za pomocą skanera mogą służyć do analizy ich właściwości geometrycznych. Wykonane pomiary ukazują, że mierzenie długości, szerokości i grubości suwmiarką i skanerem 3D daje podobne wyniki, które nie różnią się istotnie od siebie przy założonym do obliczeń poziomie istotności α = 0,05. Wyniki pomiarów i obliczeń cech geometrycznych uzyskane na podstawie zdjęć badanych owoców orzechów różnią się istotnie w większości przypadków od wyników uzyskanych za pomocą suwmiarki oraz skanera 3D. Zbudowane modele numeryczne mogą zostać zarchiwizowane i w dowolnym momencie wykorzystane do dalszej analizy kształtu za pomocą odpowiedniego oprogramowania komputerowego.
W pracy przedstawiono ocenę metody chromatografii powinowactwa immunologicznego do oznaczania sumy aflatoksyn w orzechach arachidowych z wykorzystaniem testu AFLATEST firmy Vicám. Wyznaczono parametry analityczne metody: odzysk, precyzję i dokładność. Metoda ta może być stosowana do półilościowego oznaczania aflatoksyn w rutynowej kontroli żywności.
Peanut stunt virus (PSV) is a common legume pathogen present worldwide. It is also infectious for many other plants including peanut and some vegetables. Viruses of this species are classified at present into three subgroups based on their serology and nucleotide homology. Some of them may also carry an additional subviral element — satellite RNA. Analysis of the full genome sequence of a Polish strain — PSV-P — associated with satRNA was performed and showed that it may be classified as a derivative of the subgroup I sharing 83.9–87.9% nucleotide homology with other members of this subgroup. A comparative study of sequenced PSV strains indicates that PSV-P shows the highest identity level with PSV-ER or PSV-J depending on the region used for analysis. Phylogenetic analyses, on the other hand, have revealed that PSV-P is related to representatives of the subgroup I to the same degree, with the exception of the coat protein coding sequence where PSV-P is clustered together with PSV-ER.
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