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Isolation and identification of vibrio sp. in the hepatopancreas of cultured white pacific shrimp (Litopenaeus vannamei)

100%

Shanmugasundaram S., Mayavu P., Manikandarajan T., Suriva M., Enswar A., Anbarasu R.

International Letters of Natural Sciences

2015

tom 46

The Vibrio is the most common genera with crustaceans often causing various diseases in Aquaculture and significant economic losses. Many Vibrio species are pathogenic to human and have been implicated in food borne diseases. The present study was carried out, the isolation and identification of pathogenic bacterial flora were isolated from infected in hepatopancreas of vannamei. The SPDS Oceanic farm, RS Aqua farm of and Valli vilas Aqua farm Vellar estuary, Cuddalore District, Tamil Nadu, during the period of (September 2013 to November 2013). The collected samples were plated on TCBS- (Thiosulfate-Citrate-Bile salt-Sucrose) agar medium. The present study, totally 253 green colonies were isolated from TCBS agar plates and among these, 175colonies were identified by using the biochemical tests showed the V. parahaemolyticus, V. mimicus, V. vulnificus, V. damsela and P. shigelloides. The maximum species was recorded in V. parahaemolyticus (83.4 %) and minimum was observed in V. mimicus (1.7 %).

Technika PCR i jej modyfikacje w identyfikacji patogenów ziemniaka

75%

Choluj J., Przewodowski W.

Ziemniak Polski

2014

tom 24

nr 3

Uproszczona metoda molekularnej identyfikacji nicieni z rodzaju Bursaphelenchus, należących do grupy xylophilus, z wykorzystaniem reakcji PCR ze starterami specyficznymi

75%

Filipiak A., Tomalak M.

Progress in Plant Protection

2010

tom 50

nr 1

Both native European species, Bursaphelenchus mucronatus and B. fraudulentus, are relatively common and harmless to trees. They belong to the xylophilus group, which includes also the quarantine pest B. xylophilus – the causal agent of the pine wilt disease. They can be difficult to distinguish morphologically from each other and from B. xylophilus. Therefore, reliable methods of taxonomic identification of these species are therefore of particular interest to plant quarantine services. PCR amplification with specific primers enables rapid and precise species identification, even from a single nematode. In 2004, Matsunaga and Togashi designed specific primers for B. xylophilus and B. mucronatus. The aim of this study was to design specific primers for B. fraudulentus. The specificity of the newly designed primers was tested on eight isolates of B. fraudulentus which originated from different parts in Europe (Austria, Germany, Poland, Russia and Ungarn). For all isolates, PCR amplification resulted in products of identical length of 617 bp, but no PCR products were generated for B. xylophilus and B. mucronatus. The PCR amplification with primer sets specific for B. xylophilus (XF and XR), B. mucronatus (MF and MR) and those for B. fraudulentus (FF and FR) designed in this study resulted in amplicons of different lengths (557, 210 and 617 bp, respectively), which can be easily distinguished in agarose gels.

Ograniczanie wyników

1 International Letters of Natural Sciences

1 Progress in Plant Protection

1 Ziemniak Polski

1 Anbarasu R.

1 Choluj J.

1 Enswar A.

1 Filipiak A.

1 Manikandarajan T.

1 Mayavu P.

1 Przewodowski W.

1 Shanmugasundaram S.

1 Suriva M.

1 Tomalak M.

1 2015

1 2014

1 2010

Wyniki wyszukiwania

Isolation and identification of vibrio sp. in the hepatopancreas of cultured white pacific shrimp (Litopenaeus vannamei)

Technika PCR i jej modyfikacje w identyfikacji patogenów ziemniaka

Uproszczona metoda molekularnej identyfikacji nicieni z rodzaju Bursaphelenchus, należących do grupy xylophilus, z wykorzystaniem reakcji PCR ze starterami specyficznymi