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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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An attempt to evaluate the efficacy of vaccines against pasteurellosis in rabbits

100%
Borkowska-Opacka B., Rutkowska-Jurga I., Truszczynski M., Kozaczynski W.
Bulletin of the Veterinary Institute in Puławy
|
1996
|
tom 40
|
nr 1
The goal of the studies was to evaluate the protective value of vaccines against pasteurellosis in rabbits. The vaccines comprised antigens to serotypes 3 and 12 of Pasteurella multocida with two different adjuvants: emulsigen and aluminium hydroxide gel. The two vaccines given subcutaneusly either once or twice at a dose of 1 ml protected rabbits markedly against intranasal infection with the virulent strains of examined serotypes. A substantial efficacy of these vaccines was supported by the occurrence of specific precipitins against somatic antigens of serotypes 3 and 12 present in the sera of immunised rabbits even 5 weeks after vaccination
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Effect of various adjuvants on the efficacy of the vaccines against pasteurellosis in rabbits

100%
Borkowska-Opacka B., Rutkowska-Jurga I., Truszczynski M., Kozaczynski W.
Bulletin of the Veterinary Institute in Puławy
|
1995
|
tom 39
|
nr 2
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Field trial of the active immunity in rabbits against pasteurellosis

86%
Borkowska-Opacka B., Truszczynski M., Rutkowska-Jurga I., Kedrak A.
Bulletin of the Veterinary Institute in Puławy
|
1996
|
tom 40
|
nr 1
The goal of the study was to evaluate under field conditions the protective value of the vaccines comprising antigens to serotypes 3 and 12 of P. multocida with emulsigen (Cunipastivac E) and aluminium hydroxide gel (Cunipastivac A) against pasteurellosis in rabbits. Both vaccines were given subcutaneously at a dose of 1 ml. This study revealed: 1) No harmful effects of these vaccines and a beneficial influence on body weight gains, 2) An effective protection of rabbits against the acute form of pasteurellosis in a rabbitry with a high mortality rate resulting from this disease.
4
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Use of the ELISA for determination of IgG anti-Pasteurella multocida serum level in geese vaccinated against pasteurellosis

86%
Kedrak A., Borkowska-Opacka B., Samorek-Salamonowicz E.
Bulletin of the Veterinary Institute in Puławy
|
2000
|
tom 44
|
nr 2
The ELISA was used to measure anti-Pasteurella multocida IgG levels in the serum of 30 geese collected randomly from a 300-goose flock aged 2 months and vaccinated against pasteurellosis with the Pastivac E vaccine comprising Polish isolates of serotypes 1 and 3 with oil adjuvant. The blood was sampled every 14 days for 2 months from two groups of 10 birds each vaccinated singly and doubly and from non-vaccinated considered the controls. The ELISA kits were prepared by the author's procedure because no commercial kits for goose blood were available. An increase in anti-P. multocida IgG level was found after each vaccination.
5

Immunological response to outer membrane proteins of Pasteurella multocida serotype A:3 in calves

72%
Kedrak A., Borkowska-Opacka B.
Bulletin of the Veterinary Institute in Puławy
|
2003
|
tom 47
|
nr 2
6
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Immunogenic properties of Pasteurella multocida strains isolated from rabbits evaluated in white mice

72%
Borkowska-Opacka B., Rutkowska-Jurga I., Truszczynski M.
Bulletin of the Veterinary Institute in Puławy
|
1995
|
tom 39
|
nr 2
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Application of the ELISA for determination of anti-Pasteurella multocida IgG in the sera of rabbits veccinated against pasteurellosis under field conditions

72%
Borkowska-Opacka B., Kedrak A., Truszczynski M.
Bulletin of the Veterinary Institute in Puławy
|
1997
|
tom 41
|
nr 1
The ELISA was used to measure the level of specific lgG to P. multocida in the sera of rabbits vaccinated against pasteurellosis on a farm at Ch. The vaccines comprised the antigens of serotypes 3 and 12 with two adjuvants: emulsigen or aluminium hydroxide gel. Eight-week-old rabbits were injected subcutaneously with a 1-ml dose. Animals were observed for about 2 months until slaughter. A statistically significant increase in IgG level was found in rabbits challenged one month after immunisation in comparison to non-vaccinated animals. The increase was maintained without any marked alterations for 2 months. Results indicate that the ELISA may be used to evaluate the vaccine effectiveness instead of experimental injections of animals.
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Use of the ELISA for determination of IgG anti-Pasteurella multocida serum level in rabbits vaccinated against pasteurellosis

72%
Borkowska-Opacka B., Kedrak A., Truszczynski M.
Bulletin of the Veterinary Institute in Puławy
|
1996
|
tom 40
|
nr 2
The aim of the experiment was to determine, using the ELISA and gel diffusion precipitin test, the antibody serum level in rabbits immunised with vaccines comprising P.multocida antigens of serotypes 3 and 12 with two adjuvants: oil (Cunipastivac E) and aluminium hydroxide gel (Cunipastivac A). The ELISA revealed an increase in specific lgG levels against P. multocida and the positive precipitin reaction with somatic antigens of both serotypes used for vaccine preparation. The ELISA titre did not alter during a three-month experimental period; however, the level of precipitins decreased. Comparison of the two methods revealed that the gel diffusion precipitin test is more sensitive but it is most likely that the methods used did not detect the same antibodies.
9

Wybrane choroby najczesciej zagrazajace krolikom i ich zapobieganie

58%
Kwiatanowska J.
Biuletyn Informacyjno-Handlowy. Ośrodek Doradztwa Rolniczego Boguchwała
|
2004
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nr 02
21-23
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Field examination of the boviseptivac vaccine and measurement of the IgG anti-Pasteurella multocida level by the ELISA

58%
Borkowska-Opacka B., Kedrak A., Truszczynski M., Rutkowska-Jurga I.
Bulletin of the Veterinary Institute in Puławy
|
1998
|
tom 42
|
nr 1
The goal of the experiment was to evaluate the Boviseptivac vaccine against cattle pasteurellosis manufactured from local isolates of P. multocida serotype B:2 with the addition of an oil adjuvant (Emulsigen). Field examinations were performed on 645 cows in the Ostroleka province. The vaccine effectively protected against the disease; however, 6 (93%) of examined cows revealed shock signs which were lessened with anti-shock preparations. The immunogenicity of the vaccine was determined by the agar gel diffusion precipitin test and ELISA. Examined blood was sampled from 10 cows before vaccination and 2, 4 and 8 months thereafter. The vaccine stimulated the production of specific precipitins to serotype 2 and increased levels of the IgG anti-P. multocida.
11
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Preliminary investigation into the immunisation of cows against pasteurellosis using boviseptivac vaccine

44%
Borkowska-Opacka B., Kedrak A., Truszczynski M.
Bulletin of the Veterinary Institute in Puławy
|
1997
|
tom 41
|
nr 2
Investigations aimed to evaluate the effectiveness of Boviseptivac, vaccine against bovine pasteurellosis prepared from the Polish isolates of serotype B:2 with an oil adjuvant (Emulsigen). The vaccine protective value obtained on white mice was 4.25 according to the method of Ose and Muenster. Field trials carried out on 120 cows in the Ostroleka province revealed advantageous properties of the vaccine, such as a high effectiveness and the lack of lesions at the site of injection. Moreover, no health disturbances in cows were observed. The vaccine immunogenicity was evaluated serologically. Blood collected from 10 cows was examined before vaccinaton and 1 month postinjection. Immunisation stimulated the production of precipitins specific for serotype 2 demonstrated by the precipitin diffusion reaction on agar gel. The ELISA enabled us to detect an increased lgG level after immunisation.
12

Jesienne dolegliwosci krolikow

37%
Zajac J.
Agrochemia
|
1999
|
nr 11
24-25
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