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The activity of IgG, IgM and IgE antibodies to somatic antigen of Trichinella spiralis in the sera of patients with trichinellosis at various intervals after infection was examined by means of ELISA. Mathematical analysis of the dose-response curves was used. Elevated level of IgG and IgM antibodies of relatively high avidity and of rather low IgE avidity was documented. Amount or avidity ofIgG antibodies was found to be most useful for the diagnosis of trichinellosis (85% positive results in patients' sera). The isotype of IgM avidity constitutes a better diagnostic value than the amount of it (60% and 35% of positive results, respectively).
Frequency of human parasites in selected populations of Silesian region. Epidemiological evaluation of the most frequent human parasitoses in the group of children at age of seven years, in adult patients and in group of Polish citizens coming back from tropics between 1999-2003 was performed. All examined people were Silesian region inhabitants. The biggest average infection prevalence in children from all examined years concerned Enterobius vermicularis, in adult - Giardia intestinalis, while in group of population coming back from tropics - Entamoeba histolytica-like. High percentage of anti-Toxocara canis and Toxoplasma gondii antibodies presence can result from non randomly study group recruitment.
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Wrażliwość Trichomonas vaginalis na wysychanie

75%
Twelve strains of T. vaginalis were tested for their sensitivity to dessication at room temperature. Each of them, in the quantity of 0.5 cm³ of 36 hrs old culture (containing 2.5 mln cells) was placed in 5 sterilized tubes containing 2:5 cm³ of: sterilized Roiron medium, urine, 0.9% NaCl, tap water and distilled water. After shaking, the suspension of Trichomonas of each tube in the quantity of 0.15 cm³ was placed on depression slides. The slides were then placed in sterilized, not tightly covered Petri dishes, which were kept at room temperature. The suspension of 3 slides was examined under microscope and inoculated on Roiron medium prior to complete dessication. The same procedure was followed with respect to the fourth slide but only after the suspension had, dried. The suspension remaining in the tube (2.4 cm³) served as control. The experiment showed that in a drop of 0.9% NaCl, urine and Roiron medium, Trichomonas remains active for 120 min. When transferred to Roiron medium within that time, Trichomonas reproduces in a normal way which was not observed after a transfer from the suspension dried up. In a drop of water Trichomonas loses its ability to reproduce within a time shorter than 120 min. The results speak in favour of a possibility of a non-sexual infection with Trichomonas.
The article reviews the problems on epidemiology of cryptosporidiosis and gives a detailed description of the massive waterborne outbreak in Milwaukee (Wisconsin, USA) associated with a break in filtration capacity of a public water supply. The authors emphasize the need for high-quality diagnostic procedures as current coprodiagnostic microscopical techniques seemed to fail to detect Cryptosporidium oocysts.
From 1976 to 2000, were examined 2828 children for the presence of gastrointestinal parasites. Investigations were made by means of a standard coprological methods, Graham's method and immunoenzymatic method (Prospect Giardia) for the presence of coproantigen of Giardia. The following parasites were detected in 1299 children: Giardia lamblia 1,3%, Ascaris lumbricoides 3,0%, Trichuris trichiura 7,8%, Enterobius vermicularis 40.5%. The parasites were found most frequently in children from a home for mentally handicapped. The number of infected among children inhabiting country was higher than among those from the towns. There was no significant difference between boys and girls.
Up to now, the co-occurrence of parasites sensu stricto and fungi were confirmed mostly in the orał cavity. The aim of the present parallel parasitological and mycological investigations was to determine co-occurrence of protozoa, helminths and fungi (parasites sensu lato) both in smears from cavity and in stool samples of 93 patients undergoing the non-parasitological treatment in two hospitals of Lower Silesia (Poland). Among the examined patients parasites sensu lato (13 species) were found in 62 cases (63%). Trofozoites of Trichomonas tenax and/or Entamoeba gingivalis as well as fungi (Candida albicans, C. krusei, C. tropicalis and C. pseudotropicalis) occurred in 10% of the examined patients. Almost all infected cases (90%) were joined with infection of common invasion of protozoa and fungi. In stool common invasion of parasites sensu lato (Giardia intestinalis, Entamoeba coli, Ascaris lumbricoides, Taenia sp., Enterobius vermicularis as well as Candida albicans, C. krusei, C. pseudotropicalis, C. guilliermondii and Rhodotorula sp.) were found in 12% of the patiens. The co-occurrence of parasites and fungi was stated in the half of invasions. C. albicans was the most often co-occurring species in alimentary tract.
General data in the world of Strongyloides stercoralis infection in human hosts, were presented. In addition, particular distribution of parasite in Asia, Oceania, Australia and America were described.
Przedstawiono opis procedur zaadaptowanych do izolacji i wykrywania DNA pasożytów z rodzaju Dirofilaria w materiale izolowanym od ludzi i przechowywanym w: etanolu (24 tygodnie), formalinie (46 tygodni) lub zatopionym w bloczkach parafinowych (25 tygodni). Najwyższą czułość PCR obserwowano dla próbek DNA uzyskanych z robaków przechowywanych w etanolu, a najniższą dla próbek pochodzących z materiału przechowywanego w formalinie. Izolacja DNA z pasożyta utrwalonego w formalinie była trudniejsza niż w wypadku pozostałych preparatów, a produkty jego powielania były wykrywalne tylko w Real Time PCR. Uzyskane wyniki pokazują, że metody molekularne są stosunkowo łatwym w użyciu sposobem identyfikacji nicieni z rodzaju Dirofilaria obecnych w materiale klinicznym utrwalonym na różne sposoby. Wskazane jest niestosowanie formaliny do przechowywania materiału, który ma być użyty do diagnostyki opartej o PCR.
Two commercial faecal antigen tests, based on Giardia specific antigens, were compared with traditional microscopical examination of faeces in 40 clinical patients, including 14 patients with a confirmed Giardia infection as well as in a survey among 96 children in four day care centers. The results obtained with these faecal antigen tests were not much better than by using traditional microscopy by a well trained parasitologist. Specific Giardia antigen (GSA 65) has been still detected in faeces up to day 4th after treatment of giardiosis, when microscopical examination of faeces was already for a few days negative. The Giardia antigen detection test was not very useful in examining the unconcentrated duodenal content.
Familial infections with Trichomonas tenax (O.F. Müller, 1773), Dobel, 1939. Aim of the study was to research familial occurrence of Trichomonas tenax and relationship between infection with the protozoan and both a state of parodontium and hygiene of oral cavity. The study involved 10 families (22 individuals). Trichomonas tenax was found in 16 patients. Simultaneously izolates of protozoans were examined with the methods of molecular biology. The study showed the dependence of infection with Trichomonas tenax on both bad hygiene of oral cavity and occurrence of pathological lesions in the paradontium.
The morphological and anatomical features of developmental forms of parasitic and free-living generation of Strongyloides stercoralis were described.
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