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The aim of the study was the analysis of the effect of calving season on the course of parturition and rearing results of Polish Holstein-Friesian calves of Black-and-White strain from birth to 3 months of age. Easy calvings (1;2) predominated on the farm. In spring, they accounted for 91.2% of all calvings occurring in this season. The respective values for summer, winter and autumn were 89.7%, 88.6% and 87.6%. The highest mean birth weight was characteristic of calves born in winter (40.8 kg and 44.2 kg for heifer calves and bull calves, respectively). The highest mean daily body weight gains in the whole rearing period were achieved by heifer calves born in winter (704 g) and bull calves born in spring (750 g). In the group of heifer calves and bull calves, statistically significant differences during the analysed rearing period were found (P≤0.01, P≤0.05). The highest percentage of ill calves was found in the autumn-winter season and the lowest one in spring and summer.
To examine the changes in dentine microstructure in spotted souslik Spermophilus suslicus (Giildenstaedt, 1770) during pregnancy, parturition and lactation, we investi­gated 39 females from the Odessa region. Thirty females were dead-trapped in March­-July, 1987-1997, nine females (lactating or pregnant) were caught in April, 1996, injected with tetracycline as a time marker and kept in captivity during 14-47 days. Their litters survived from 1 to 29 days. We analyzed cross stained sections as well as cross ground sections of upper incisors. In the lactating sousliks both in nature and in captivity the daily layers formed in the course of pregnancy were followed by formation of a specific dentine pattern. The pattern consisted of alternating contrastive hypo- chromatic and hyperchromatic bands of about 30-50 n wide. The beginning of the pattern formation coincided approximately with parturition and the formation con­tinued during lactation. Therefore, the parturition-lactation zone could be the correct name for the pattern. In captured females no correlation between the duration of the pattern formation and the lifespan of their litters was found. The pattern was absent in immature and barren females. We found no changes in the dentine formation during pregnancy.
The activity of monoamine oxidase (MAO) in cow serum undergoes considerable fluctuations in the course of the oestrous cycle. The aim of the study was to determine the activity of MAO and its isoenzymes A (MAO-A) and B (MAO-B) in cow serum near the parturition. The experiments were performed on 8 pregnant and clinically healthy cows of the Polish Black and White Breed. Peripheral blood samples were collected every 4 hours before, during and after parturition. MAO serum activity was determined with the use of the spectrofluorimetric technique according to Matsumoto et al. (1985) with own modifications. Analysis of serum samples showed that MAO activity increased rapidly near the delivery. MAO-B activity was significantly higher than MAO-A activity and determined the profile of total MAO (MAO-T) activity. It may be suggested that an increase in MAO activity observed near delivery protects against an excessive elevation of catecholamine concentration in blood. However, the control mechanism and physiological role of MAO during this period need further investigations.
On dairy farms with high milk production there are much health problems. One of the most important periods, the critical time in dairy cows production is calving. In controlling and resolving of these problems the great role have the farmer and the veterinarian. There are many varied methods of dairy farms supervising. Diagnostic monitoring in herds of dairy cattle has an important role in controls an acceptable level of health and productivity in the cows and their progeny. Being familiar with the internal environment of the animal enables early detection of any dangers to the health of the animal and enables one to react before any clinical signs appear. Thus management in periparturient dairy cows is very important for prevention of many disturbances. The aim of the study was to compare the values of selected blood parameters in dairy cows shortly pre and post partum. It had to answer on the question The materials consisted of 30 clinical healthy cows tested 3-7 days before calving and 3-5 days after calving in this same good environmental and nutritional conditions (borning room). Selected twenty one various biochemical and morphological parameters of the blood similar to standard metabolic profile were included in the study. Significant differences (α=0.05) were noted in total bilirubin, SGOT, inorganic phosphorus, Calcium and Chloride. Parturition is an important period in cows from the point of view of the physiological changes taking place which in turn produce measurable, significant changes in the diagnostic parameters of the blood. These differences are presented in the paper.
In clinical practice, the parturition date in bitches is calculated on the basis of the established ovulation date, using determination of the level of LH and P4. Additionally cytological examination of vaginal smears makes it possible to establish the onset of diestrus, from which the delivery occurs after about 57 days. The latest method used in bitches for this purpose is foetal biometry. Measurements of the inner chorionic cavity diameter (ICC) and biparietal diameter (BP) with formulas published by Luvoni and Grioni have been popularized and the most practically useful. In a group of six bitches the effectiveness obtained with the use of ICC was 66.67% with an accuracy of 1 day and 100% with an accuracy of 2 days. BP measurements gave better results, as the accuracy was respectively 83.33% and 100%. The effectiveness of predicting the delivery date on the base of method consisting in determining the LH surge was 66.67% with an accuracy of 1 day and 100% with an accuracy of 2 days. The best effectiveness was obtained while using the method based on P4 level determination (100% both with an accuracy of 1 day, as well as of 2 days). The lowest accuracy was obtained while using the cytological method. Generally, a comparison of the methods used for predicting the date of delivery in bitches confirms practical usefulness of fetometry. Its effectiveness was not lower than traditional methods used so far, while its advantage lies in the ease of performance and the long term of application during gestation. However, full confirmation of the practical usefulness of this method requires further research on a larger number of animals.
The aim of the study was the determination of IgA, IgM and IgG concentrations in porcine serum and colostrum, in order to evaluate their variations in the perinatal period, as well as to clarify whether there is a correlation between colostrum intake, initial level of immunoglobulins (Ig) in piglet serum and development of their own immunity. The mean IgA, IgM and IgG concentrations in sow serum 10 days before parturition were 1.58, 6.12 and 39.56 mg/ml, respectively. Seven days later only the IgG level was insignificantly lower (34.94 mg/ml, p=0.55), while concentrations of IgA and IgM increased to 2.25 and 7.25 mg/ml, respectively (p=0.23 and 0.62, respectively). The mean initial IgG concentration in colostrum at farrowing was 118.5 mg/ml and differed between sows. The average value of IgA in colostrum at birth was 23.8 mg/ml and decreased to 7.85 mg/ml at 6 hours (h) and to 4.59 mg/ml at 24 h after the onset of farrowing. IgM concentration at birth was 12.1 mg/ml and decreased to 4.23 mg/ml at 24 h postpartum. Positive relationships were found between concentrations of IgM and IgA in serum of piglets at 14 and 56 days of life (r = 0.41 and 0.80, respectively, p≤0.05) as well as for IgG concentration in the piglets serum at 7 days and 56 days of age (r = 0.48, p≤0.05). The above observations suggest that there is a correlation between the level of Ig in piglet serum in the first days of life and improvement of their own immunity.
Oxytocin (OXY) has been shown to attenuate some of the physiological and behavioral alterations appearing in stressed rats. Carbetocin (CBT), an oxytocin analog [deamino-1-monocarba-(2-O-methyltyrosine)-oxytocin], was designed to exert prolonged action. In the present study we investigated the impact of these peptides on the behavioral changes in rats exposed repeatedly to restraint stressors. Wistar male rats were exposed to restraint for 1 hour; saline or drugs were administered intraperitoneally immediately after stress termination. Recording of the exploratory activity in the open-field started 60 min later. To explore the possibility of persisting effects of stress and/or drugs, the procedure was repeated for three consecutive days. Restraint moderately suppressed locomotion and rearing, and increased grooming. OXY in 0.3 mg/kg dose showed a tendency to restore the suppressed exploratory activity. In contrast, 1 mg/kg dose potentiated the stress-induced behavioral deficit. Both OXY doses slightly increased grooming. CBT in the same two doses restored the stress-induced deficits in locomotion and rearing but did not influence grooming. The locomotor depression after 1 mg dose of OXY was found also in non-stressed rats in contrast to the increased activity after CBT. The data support the view that post-stress administered CBT exerts a significant effect on the stress-altered spontaneous behavior.
The length of gestation in Carthusian broodmares was calculated on the basis of 339 spontaneous full-term deliveries taking place in the 8-year period 1998-2005 from 158 broodmares and 29 stallions in a major farm of Spanish horses of Carthusian strain in southern Spain. Ultrasonography was used to determine follicular dehiscence, 1st day of pregnancy and to confirm conception in mares. Mean GL was 332.4 ± 12.1 days, and a normal interval of 297-358 days was established for this breed. GL records were grouped on the basis of foal sex (colts or fillies), mating month (between November and January; February and April; May and July), age of the mare (4 to 7 years; 8 to 12 years; 13 to 17 years), breeding year, stallion and parity (primiparous vs. multiparous). GLs were 12.9 days shorter in mares mated between May and July than those mated between November and January and 15.3 days in mares mated between February and April (p<0.001). Mares aged between 8-12 years had 5.3 days shorter GLs than those aged between 13-17 years (p<0.05). Pregnancy was significantly 5.7 days longer when the mare gave birth to colts than fillies (p<0.05). GL was 14.5 days longer in primiparous than in multiparous mares (p<0.001). No statistical differences in GL were found between the studied years. This study shows the influence of certain stallion on GL.
The aim of the study was to evaluate oxidative stress in cows undergoing normal parturition and cows suffering from dystocia. The erythrocytic glutathione peroxidase (GSH-Px) activity, plasma vitamin A and ß-carotene concentrations, and paraoxonase - PONI activity were lowered (P0.001, P<0.01, 0.05, and 0.05, respectively) in cows with dystocia compared to normal calving cows. The erythrocytic malondialdehyde (MDA) concentration was markedly increased in the dystocia group compared to normal calving group. However, erythrocytic glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) activites, and plasma vitamin E and MDA concentrations were not significantly changed in the dystocia-affected cows in comparison to eutocia cows. In the difficult calving cows, there were significant correlations between the activities of GSH-Px and SOD (r=-0.4l, P<0.01), plasma ß-carotene levels and paraoxonase activity (r=0.34, P<0.05), body condition score (BCS) and plasma MDA (r=0.46, P<0.05). Similarly in the eutocia cows there were significant correlations between CAT activity and MDA concentration (r=-0.76, P<0.01), levels of plasma ß-carotene and PONI (r=0.58, P<0.01), BCS and MDA concentrations (r=0.50, P<0.05), and BCS and vitamin E (r=0.53, P<0.05) concentrations. These results suggests that evaluation of plasma vitamin A and ß-carotene concentrations, PONI and GSH-Px activities, and MDA concentration seems to be useful in the assessment of dystocia in cows.
Eighteen purebred Arabian mares and six Anglo-Arabian mares were observed during first 4 h after foaling in one of the leading Polish stud farms in two foaling seasons. The aim of the research was to analyse the behaviour of mares and the condition of foals after parturition. Specific behavioural responses like licking, nuzzling, and touching a foal were identified as interactive activities supporting the process of creating the bond between the mare and the foal. The maternal behaviours started immediately after parturition and reached the biggest frequency in the first hour after foaling. The time spent by the mares on standing, resting, and eating has also been measured. The results of observations were compared in reference to mares' breeds and statuses. Aggressive maternal behaviour, both normal and abnormal (perceived as aberration) was noticed. Foal's condition after parturition was assessed on the basis of the first standing up, colostrum nursing, and meconium passing. On average, the foals made attempts to stand up in the 21st min after birth, and the first self-reliant getting up occurred in the 85th min. The share of time spent by the foals in the upright position increased with even hour. Furthermore, the time of the first colostrum nursing was associated with the first standing up. The duration of gestation influenced foals' colostrum nursing and meconium passing.
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