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Effect of cAMP analogues on glomerular inulin space of isolated rats renal glomeruli

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Van Bemmelen M. X. P., Szczepanska-Konkel M., Jastorff B., Jankowski M., Angielski S.

Journal of Physiology and Pharmacology

2005

tom 56

nr 1

Cyclic AMP has been generally recognised as activator of cAMP-dependent protein kinases. However, there is little evidence about role of cAMP-dependent protein kinase (PKA), in particular izoenzymes PKA-I and PKA-II, in glomeruli contractility. We measured changes of glomerular inulin space (GIS) as a marker of its contractility in the presence of phosphodiesterase resistance cAMP analogues; activators and inhibitors of PKA. Activator of PKA i.e. (Sp) 8-Cl-cAMPS (0.1-100 µM) decreased GIS. (Rp) 8-Cl-cAMPS (0.1-100 µM), inhibitor of PKA, was ineffective but shifted concentration-response curve of (Sp) 8-Cl-cAMPS to right at 50 µM. Specific A site activation by N6-benzoyl-cAMP decreased GIS with maximum at 0.1 µM. Activation of B site by 8-aminobutyloamino-cAMP (0.1-100 µM) had no effect. However, specific activation of both sites on PKA-I or PKA-II by site-selective analogue pairs e.g. 8-aminobutyloamino-cAMP plus 8-piperidino-cAMP or N6-benzoyl-cAMP plus 8-piperidino-cAMP respectively, significantly increased sensitivity of glomeruli to analogues. Our data suggest that activation of PKA-I or PKA-II might have an important role in the regulation of glomerular contractility.

Ograniczanie wyników

1 Journal of Physiology and Pharmacology

1 Angielski S.

1 Jankowski M.

1 Jastorff B.

1 Szczepanska-Konkel M.

1 Van Bemmelen M. X. P.

1 2005

Wyniki wyszukiwania

Effect of cAMP analogues on glomerular inulin space of isolated rats renal glomeruli