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Nowe rodzaje szczepionek przeciwpasozytniczych

100%

Wedrychowicz H.

Wiadomości Parazytologiczne

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2000

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tom 46

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nr 1

21-27

The protection of humans and domestic animals against parasitic infections remains a major goal, especially in light of developing of drug resistant strains in many parasite species. "Classic" vaccines are based on attenuated infective stages of protozoan and helminth parasites. Although such vaccines are effective in confering host immunity against several Protozoan (coccidiosis, giardiosis, toxoplasmosis) diseases and one helminth (dictyocaulosis) they are very unstable and expensive. Recombinant techniques enable to obtain protective antigens quickly and in considerable quantities, cultivating of the bacteria and purification of the recombinant protein is less expensive than the maintenance of host animals and isolation of the protective antigens from harvested parasites. Moreover, the cloned protective antigens may be deprived of epitopes responsible for immunopathology. However, at present only one anti-parasite recombinant protein vaccine is commercially available (TickGARD). Such a situation may result from that many protective parasitic antigens cannot be expressed in bacteria or yeast in anative from. DNA vaccines present many advantages over protein ones. Firstly, the antigenic proteins synthesised within the host cell possess an appropriate molecular structure and undergo a posttranslational modifications specific for a native protein. The next advantage of DNA vaccines is that DNA is easier to handle and more resistant than proteins to temperature changes. DNA vaccines are likely to induce novel mechanisms of i mm une response, which may be beneficial in case of parasitic invasions. Costs of DNA vaccines are comparable, and may be even lower, in comparison to recombinant protein vaccines. The main obstacle preventing the use of DNA vaccines is still Jack of the complete knowledge conceming mechanisms of their action. Vaccines based on transgenic plants (=edible vaccines), expressing the protective parasitic antigens, present another promising approach in research on anti-parasitic vaccines. Such vaccines may be of special importance in prevention of infections with gastrointestinal parasites.

2

Witold Stefanski - uczony, wychowawca, czlowiek

80%

Fagasinski A., Tarczynski S.

Wiadomości Parazytologiczne

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1992

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tom 38

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nr 1-2

3-7

3

Molekularne mechanizmy lekoopornosci pasozytow

80%

Majewska A. C.

Wiadomości Parazytologiczne

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1998

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tom 44

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nr 2

139-150

In spite of the enormous achievements of medical and biological sciences, parasites still constitute important medical and veterinary problem. One of the main obstacles in the treatment and eradication of human and animals parasitic diseases is drug resistance of parasites. This phenomenon has more and more frequently been described both in medical and veterinary practice. The application of numerous molecular biology techniques in modern parasitological laboratories have enabled the investigations of parasites at the DNA level. The results of these studies have revolutionised our knowledge and made possible the recognition of not only the reasons and extent of genetic differentiation of parasites but also the recognition of genetic and molecular mechamisms of drug resistance. The results of many studies showed interspecific, interpopulational, and even intrapopulational heterogeneity of parasites in their sensitivity to antiparasitic drugs. Moreover, it has been found that in various parasites there often exist different molecular bases of the drug resistance. The review concerns different molecular mechanisms of drug resistance in selected parasite taxa (Schistosoma spp., Haemonchus contortus, Leishmania spp., Plasmodium falciparum, Entamoeba histolytica).

4

Wykrywanie koproantygenow w chorobach pasozytniczych

80%

Kasprzak W.

Wiadomości Parazytologiczne

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1995

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tom 41

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nr 1

19-24

The author presented general issues in detecting of parasite's coproantigens and carried out a critical review of abstracts submitted to the scientific session (17th Congress of the Polish Parasitological Society, September 1994, Gdynia, Poland). The abstracts are being published in a special edition of the "Methodical and Organisational Bulletin" (Institute of Marine and Tropical Medicine, Gdynia, Poland).

5

Zastosowanie technik biologii molekularnej w parazytologii: za i przeciw

80%

Majewska A. C., Sulima P.

Wiadomości Parazytologiczne

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1998

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tom 44

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nr 2

181-194

This review deals with possible advantages and disadvantages connected with applying molecular biology techniques in parasitology. It particularly concerns molecular diagnosis and epidemiology, as well as phylogenesis and taxonomy of selected parasite species.

6

Sprawozdanie z Miedzynarodowej Konferencji Parazytologow [ICOPA XI]

80%

Doligalska M.

Wiadomości Parazytologiczne

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2007

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tom 53

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nr 2

161-163

7

Profesor Jadwiga Zlotorzycka. Jej wklad w nauke, zycie uczelni oraz dzialalnosc Polskiego Towarzystwa Parazytologicznego

80%

Lonc E.

Wiadomości Parazytologiczne

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1998

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tom 44

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nr 1

114-118

8

Wirusy pasozytniczych pierwotniakow

80%

Kasprzak W., Majewska A. C.

Wiadomości Parazytologiczne

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1995

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tom 41

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nr 2

131-137

The authors present the actual review on several publications concerning the molecular characterizations of the viruses found in parasitic protozoa such as Giardia, Trichomonas, Leishmania and Entamoeba histolytica. All of the RNA viruses observed in parasitic protozoa showed several similarities and did not considerably differ from the viruses found in simple eukaryotic cells; they closely correspond to dsRNA viruses of yeast. The supposition that the protozoan symbionts detected in laboratories transfer to their hosts in natural conditions seemed to be rational, though, there are no evidences that these symbionts are potential pathogens. However, the opinion reiterates that intestinal protozoa (e.g. Entamoeba histolytica) may serve as vectors for HIV or cofactors of HIV infection. The authors point out that irrespective of the potential role of viruses as vectors in the transfection system for parasitic protozoa, the observed viral system constitutes an unusual experimental system to solve the problems of gene expression.

9

Zastosowanie analizy restrykcyjnej dla celów parazytologii

80%

Wandurska-Nowak E.

Wiadomości Parazytologiczne

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1989

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tom 35

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nr 4

Application of analysis of nucleic acid using restriction enzymes in parasitology is discussed. The main advantage of the new technique is the possibility of direct and detailed studies at the level of DNA. At present, the "genetic probe" becomes more and more commonly applied to indentification of both parasites and their transmitters. It appears that the technique of restriotion analysis is of great significance for solving taxonomic problems in parasitology.

10

Szybka metoda wykonywania semipermanentnych glicerozelatynowych preparatow z pasozytow

80%

Rolbiecki L.

Wiadomości Parazytologiczne

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2002

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tom 48

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nr 1

87-88

A rapid method for preparing semipermanent glycerol-jelly parasite mounts. A rapid method for preparing semipermanent glycerol-jelly mounts of monogeneans, digeneans, nematodes, acanthocephalans, and crustaceans is presented.

11

Trichinellosis in France [1988-1990]

80%

Soule C.

Wiadomości Parazytologiczne

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1992

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tom 38

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nr 3-4

153

12

Babesjoza - trudnosci diagnostyczne

80%

Cichocka A., Skotarczak B.

Wiadomości Parazytologiczne

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2001

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tom 47

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nr 3

527-533

Human babesiosis is caused predominantly by B. microti and B. divergens, a protozooan parasites of red blood cells. Both are transmitted by Ixodes ricinus ticks, also the primary vector of Lyme disease. Clinical manifestation varied widety from asymptomatic infection to a serve rapidly fatal disease. The diagnosis of babesiosis include examination of stained blood smers, serological evaluation indirect antibody tests and PCR. With the evolution PCR - based techniques, the diagnosis and monitoring of babesial infections became more sensitive and reliable.

13

Toxoplasma gondii - wewnatrzkomorkowy pasozyt

80%

Dlugonska H.

Wiadomości Parazytologiczne

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2002

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tom 48

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nr 1

23-28

Toxoplasma gondii -intracellular parasite. Tue article presents selected data concerning invasion and intracellular life of obligate intracellular parasite Toxoplasma gondii in susceptible bosts.

14

Diagnostyka ludzkiej anaplazmozy granulocytarnej

80%

Grzeszczuk A., Stanczak J., Pogorzelska J., Prokopowicz D.

Wiadomości Parazytologiczne

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2005

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tom 51

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nr 2

109-114

Diagnostics of human granulocytic anaplasmosis. Human granulocytic anaplasmosis is one of recently emerging tick-borne diseases. The paper summarizes current opinions on diagnostics of this zoonosis, including the recent recommendations of ESCAR (European Society of Clinical Microbiology and Infectious Diseases Study Group on Coxiella, Anaplasma, Rickettsia and Bartonella).

15

Immunoprofilaktyka w parazytologii

80%

Kolodynski J., Okulewicz A.

Wiadomości Parazytologiczne

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1998

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tom 44

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nr 2

203-215

In the paper chosen problems of immunoprophylaxis against parasitoses were presented, as well as an extensive review of references concerning the vaccines, either being already produced or being under examination in laboratories, aga.inst parasitic Protozoa and Metazoa.

16

Prof.dr hab. Wieslaw Jan Chowaniec [wspomnienie posmiertne]

80%

Ziomko I.

Wiadomości Parazytologiczne

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1994

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tom 40

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nr 2

215-217

During the years 1992-1993 terrestrial snails were examined to the infection with larvae of Protostrongylidae family in the South and North of Poland. Using compressor method 2267 terrestrial snails from two areas (Pszczyna and Iława) were examined. Collected gastropods belonged to the following species: Arion subfuscus (45 specimens), Bradybaena fruticum (9), Cepaea nemoralis (29), Succinea putris (2147), Zonitoides nitidus (37). Larvae of three species of Protostrongylidae family were found: Elaphostrongylus cervi, Varestrongylus sagittatus and V. capreoli. The highest extensiveness of invasion of Protostrongylidae was noticed in Succinea putris and reached 23,5% in one of plots in the Iława Forest District. The highest intensity of infection was found in S. putris and was as follows: E. cervi up to 150 larvae, V. sagittatus up to 41, and V. capreoli up to 132 larvae. Besides Z. nitidus (1 specimen) there were not noticed any larvae of Protostrongylidae in other species of terrestrial snails. Average extensiveness of invasion of Protostrongylidae in 1992 and 1993 amounted to 7,2 and 9,5% in Iława, and 13,9 and 9,4% in Pszczyna, respectively. The highest percentage of infested gastropods was noticed in June and July. Similar results were obtained in both biotopes examined.

17

Mikromacierze DNA i ich wykorzystanie w parazytologii i medycynie

80%

Jaros S.

Wiadomości Parazytologiczne

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2006

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tom 52

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nr 1

13-29

The article presents the current knowledge on the microarray technique and its applications in medical sciences and parasitology. The first part of the article is focused on the technical aspects (microarray preparation, different microarray platforms, probes preparation, hybridization and signal detection). The article also describes possible ways of proceeding during laboratory work on organism of which the genome sequence is not known or has been only partially sequenced. The second part of the review describes how microarray technique have been, or possibly will be, used for better understanding parasite life cycles and development, host-parasite relationship, comparative genomics of virulent organisms, develpoment vaccines against the most virulent parasites and host responses to infection.

18

Organelle wydzielnicze Toxoplasma gondii - ich rola w procesie penetracji i zasiedlania komorek zywiciela.

80%

Gatkowska J. M.

Postępy Biologii Komórki

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2003

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tom 30

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nr 3

515-524

19

Kryptosporydioza u osob zakazonych HIV

80%

Majewska A. C., Sulima P., Werner A., Baralkiewicz G., Juszczyk J., Pieniazek N. J.

Wiadomości Parazytologiczne

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1999

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tom 45

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nr 2

125-128

Cryptosporidium parvum is a coccidian parasite that affects millions of people worldwide. Clinical outcome of human cryptosporidiosis differs between immunocompetent and immunodeficient individuals. C. parvum is responsible for causing protracted and life-threatening diarrhea, biliary, and pulmonary infections in immunocompromised persons, especially in patients with AIDS. Though no effective treatment has been found so far, early diagnosis may be useful in controlling the infection. Thirty-eight stool specimens obtained from 35 HIV-positive patients admitted to the Clinic of Infectious Diseases in Poznań, Poland, were examined for the detection of oocysts, coproantigen and DNA of Cryptosporidium using standard microscopic, immunologic and molecular diagnostic methods. The presence of Cryptosporidium was detected in 10 HIV-positive patients. Oocysts, coproantigen and DNA of this parasite were identified solely in one specimen while Cryptosporidium DNA was detected in 8 specimens. Cryptosporidium coproantigen was found only in one sample. Although, the PCR was the most useful technique in the detection of Cryptosporidium in HIV-positive patients it should be noted that PCR has many pitfalls and needs to be carefully controlled to avoid both false positive and false negative results.

20

Prof. dr hab. Teresa Sulgostowska - nestorka polskiej parazytologii

80%

Grytner-Ziecina B.

Wiadomości Parazytologiczne

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2010

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tom 56

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nr 4

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