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We report a case of a male cadaver aged 72 years with an ectopic location of the papilla of Vater. The ectopic papilla was situated at the supero-posterior border of the 3rd portion of the duodenum at a distance of 0.9 cm from the limit of the 2nd and 3rd portions of the duodenum. The frequency of this anomaly fluctuates between 0 and 11.83% and when the papilla is located distal to its usual position the usual location is in the proximal 2 cm of the 3rd part of the duodenum. We refer to the possible difference in the papilla’s location between patients and cadavers and call attention to the differential diagnosis with spontaneous or surgical fistulae.
Early Jurassic aragonitic foraminifers are outstandingly well-preserved in the Marmorea crust, a multiphased ferromanganese layer limiting the Schnöll and Adnet formations (Adnet, Northern Calcareous Alps, Austria). This remarkable preservation, related to the pervasive impregnation of aragonitic tests prior to their recrystallization, allowed observing unknown diagnostic features of the genus Involutina, which typifies the Suborder Involutinina. Thanks to a detailed examination of the Adnet specimens, this paper clarifies the taxonomy, systematic position, and phylogeny of Involutina. A new diagnosis, structural model, and lineage are introduced for the group. Involutina is the direct descendant of Aulotortus and the two taxa probably showed a parallel evolution. As Aulotortus, Involutina presents a high intraspecific variability and its diversity must be revised downward. Current phylogenetic and taxonomic frames of the Suborder Involutinina are firmly questioned as, contrary to previous schemes, the type-genus possesses more than one lamellar deposit per whorl. In Involutina, the height and distribution of papillae on the test surface is not random and probably related to a biological function. We here propose that the papillose lamellae and tube infoldings that characterize representatives of the genus were rudimentary features for light catching and symbiont positioning, respectively.
The distribution and three-dimensional structure of the lingual papillae were studied by means of scanning electron microscopy. The elongated tongue in the bank vole is about 12 mm in length and about 3 mm in width. The characteristic features of the tongue are the median sulcus on the apex of the tongue, considerable narrowing in the body of the tongue and a well developed intermolar prominence. On the surface of the apex and body of the tongue three morphological types of the filiform papillae and fungiform papillae were observed. The intermolar prominence of the tongue is covered with conical and saw-like filiform papillae. On the posteriolateral margin of the intermolar prominence two foliate papillae were found. A single oval vallate papilla was situated in the median line of the anterior part of the root of the tongue. The posterior part of the lingual root is flat without papillae. The distribution and types of the lingual papillae found in the bank vole are similar to those in species of the Microtinae family.
Examinations were conducted on four tongues of adult rabbits (Oryctolagus cuniculus). On the basis of observations in a scanning electron microscope (SEM) it was found that fungiform papillae in the rabbit are located on the dorsal surface of the apex and body of the tongue, in the front of the torus of the tongue and on the margins of the tongue. Moreover, fungiform papillae are located laterally in relation to the torus of the tongue and are arranged linearly. Fungiform papillae are distributed among filiform papillae and are separated from them by the interpapillary epithelium. The connective tissue core of fungiform papillae is formed from the body, narrow at the base and wider at the apex and 10-17 crest-like folds arranged around them. On the dorsal surface of the connective tissue core of fungiform papillae there are impressions of taste buds and occasionally traces of lymphatic nodules.
Rudiments of fungiform papillae were observed at day 18 of prenatal development. They were arranged only on the dorsal surface of the apex of the tongue and its margins. The fungiform papillae looked like a hemispherical, dome-shaped eminences. From day 22 of prenatal development, scanning electron microscope showed also that rudiments of fungiform papillae appeared on the body of the tongue at the front of forming torus linguae, and on its both sides. No rudiments of filiform papillae were observed at day 22 of prenatal development. However, after removal the epithelium, the connective tissue cores of rudiments of fungiform and filiform papillae were visible. Scanning electron microscopy showed on the surface of the tongue numerous filiform papillae at day 26 of prenatal development. They were arranged on the entire dorsal surface of the apex and body of the tongue, and on its margins. During morphogenesis two types of filiform papillae were noticed. One type of filiform papillae was similar to separated cones, another had 3–4 processes more. The similar changes was with the shape of connective tissue core. The fungiform papillae were located among filiform papillae. Scanning electron microscope showed three types connective tissue cores of developing fungiform papillae. One type was mace-shaped, another one was bowl-shaped and yet barred-shaped. At day 30 of postnatal development on the surface of the connective tissue cores of fungiform papillae, from its top towards its base there were parallel and longitudinal folds.
The biology of flowering and the micromorphology of Cornus alba flowers were studied using light and scanning electron microscopy. The flowering of white dogwood in 2008 lasted 35 days, and the lifespan of a single flower was 3 days. The number of flowers per inflorescence was variable (on the average, it was 89). The largest group of insects visiting the flowers of C. alba comprised Hymenoptera (mainly bees and andrenids), then ants, dipterans and beetles. They foraged the dogwood flowers most intensively between 11.00 and 15.00. The inconspicuous four-petalled flowers of C. alba were characterised by the occurrence of T-shaped, two-armed non-glandular trichomes covering the receptacle as well as observed on the petals of the corolla, the style of the pistil and the anthers in a smaller number. The trichomes were covered by a thick cuticle with characteristic outgrowths. They contained a living protoplast, and plastids were observed in the cytoplasm of the trichome cells. In addition, anomocytic stomata were found in the epidermis of the receptacle and in the epidermis of the corolla petals. The stigma of the pistil and the adaxial epidermis of the petals were composed of very numerous conical papillae.
The structure of the fl oral nectaries of Lonicera kamtschatica was examined using light microscopy, scanning electron microscopy and transmission electron microscopy. Nectariferous tissues are located in the lower portion of the corolla tube. It was found that the secretory tissue of the nectary was composed of two layers of epidermal formations: short papillae and about 3x longer unicellular trichomes. They cover the adaxial surface of a small spur. Nectar secretion takes place through the apical portion of the trichomes and papillae. The cell wall of the upper part of the trichome has protuberances participating in nectar transfer to the subcuticular space which reaches large dimensions. The lateral walls of the trichomes are saturated with cutin. The papillae have much thicker walls than the trichomes. In the papillae, there are no wall protuberances. Less secretion accumulates in the subcuticular cavities of the papillae than in the trichomes.
The present study, carried out in the period 2008 – 2009, covered some morphological and anatomical features of the flowers of Polemonium caeruleum L. and their nectar production rate in the climatic conditions of the Lublin region. Observations were made with stereoscopic, light and scanning electron microscopy. Nectar production in the flowers was determined using the pipette method. The flowers of Polemonium caeruleum develop a calyx covered by an epidermis with numerous non-glandular and glandular trichomes. The secretory trichomes are composed of a several-celled stalk and a four-celled head. At the boundary of the corolla tube and the lobes, there is white colouration with violet nectar guides. The epidermis in this region produces several-celled living trichomes that close the entry into the corolla tube, thereby protecting the nectar accumulated in it. These hairs are at the same time glistening colour attractants for insects. The tripartite stigma of the pistil is covered, from the adaxial side, by unicellular papillae with striated cuticular ornamentation, growing at high density. Around the ovary there is located a nectariferous disc, in the form of a free projection, which secrets nectar with sugar concentration of 29 – 52% and sugar weight ranging 1.1 – 1.8 mg/flower.
The comparative analysis of leaf morphology was performed in E. helleborine (L.) Crantz, E.atrorubens (Hoffm.) Besser, and their interspecific hybrid, Epipactis ×schmalhausenii Richt. The aim of this research was to find out features that would be useful in the taxonomy of the genus Epipactis, and particulary their taxa of hybrid origin. In course of the studies special attention was paid to leaf morphology, mostly to their margins, presence of papillae, their shape and size, and to leaf structure. The thickness of the leaf blades manifested by number of spongy mesophyll cell layers, size of vascular bundles and the level of leaf sclerification were compared in the hybrid and both parental species. In E. helleborine and E. atrorubens the analyzed features were highly variable, especially in the former species, which is characterized by wide phenotypic plasticity. Morphological and anatomical features in E. ×schmalhausenii showed a transitional character to the parental species. Our results suggest that a single feature can not be of taxonomic value, but the combination of different traits has to be considered when distinguishing parental and hybrid taxa.
The conducted studies pertained to micromorphology of the surface of epidermis cells and histological traits of staminal filaments of Asphodelus aestivus Brot, flowers. The structure of the filaments was analyzed in a light microscope (LM) using various histochemical techniques. The morphology of the surface of the epidermis of filaments was observed in scanning electron microscope (SEM). Filaments Asphodelus aestivus accrete together with the basal part of the abaxial surface with the leaves of perianth. Their lower, wider, and flattened part surrounds the ovary. The epidermis of the staminal osmophores creates papilliose cells and unicellular hairs of various sizes. In the uppermost part of these structures, round marks in the cuticle layer after the emission of discharge were observed with the SEM. The outside, convex wall of the isodiametric cells of the epidermis, papillae and hairs was significantly thicker from the remaining walls. It was covered with cuticle of different ornamentation. The cells that created papillae and hairs had a large, centrally located vacuole and a thin layer of cytoplasm with numerous small vacuoles as well as large, often lobed nuclei. In the protoplasts of these cells the presence of plastids and lipid droplets was noted. During the time of secretion of elicitor between the wall and cuticle of the epidermis cells, convex bubbles were formed, in which the secreted substance was accumulated. At the end of secretion, on the surface of papillae, hairs and other cells of the epidermis, irregularly protrading cuticle was observed. It was noted that the composition of staminal osmophores in the flowers of Asphodelus aestivus includes papillae, hairs and cells of the epidermis that do not form papillae.
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