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Three seed priming techniques: hydropriming, halopriming and osmopriming, were compared for their effects on germination and vigour of pansy (Viola × wittrockiana Gams.) seeds at 20°C, 30°C and 35°C. Seeds were hydroprimed in the restricted volumes of water (600 and 700 μl H2O·g seed-1, 2, 3 or 4 days), haloprimed in KNO3 solution (-1.5 MPa, 5 days), and osmoprimed in polyethylene glycol solutions (-1.0, -1.25 or -1.5 MPa PEG 8000, 7 days) at 15°C or 20°C. Seed germination and vigour tests were performed for untreated and primed seeds. Generally, hydropriming negatively affected the speed of germination, the percentage of germinating seeds and germination capacity. Halopriming accelerated seed germination at 20°C, 30°C and 35°C but did not influence the percentage of germinating seeds and germination capacity. Osmopriming of seeds in PEG solution of osmotic potential –1.0 MPa at 20°C not only improved germination rates at 20°C, 30°C, and 35°C to the highest extent, but also increased percentage of germinating seeds at 30°C and 35°C most effectively and positively affected seed germination capacity at 20°C and 30°C.
Phytophthora cactorum was detected on &9/10; of pansies showing yellowing of leaves and crown rot symptoms and constituted about 90% of isolates obtained. Botrytis cinerea, Fusarium avenaceum, F. solani and Pythium ultimum were also isolated from diseased tissues. Using rhododendron leaves as the bait, P. cactorum was detected in pansy substratum as well as from soil under the mata. Isolates obtained from diseased plants, substratum and soil under mata colonized leaves, stem parts and roots of pansy. Necroses spread faster on organs inoculated with cultures from plants and substratum. Among 25 cultivars inoculated with P. cactorum, disease symptoms did not occur on 3 of them, whereas the fastest spread of necrotic spots (3.8 mm/24 hrs) was noticed on 3 cultivars. Isolates of P. cactorum from Begonia semperflorens and Malus domestica colonized leaf petioles of pansy with significantly faster spread when isolates from begonia and pansy were used for inoculation.
The aim of the present study was to determine the effect of an herbal supplement used in feed mixtures on the selected physical features and the chemical composition of broiler chickens’ bones in reference to antibiotic growth promoter. The experimental material was provided by 180 ROSS broiler chickens. One-day-old nestlings were divided randomly into 2 control groups and 4 experimental groups, in three replications, 10 birds each. The feed mixtures (Starter and Grower/Finisher types) were made of corn and extracted soy meal and they also contained fodder yeast, soy oil, mineral additives, premixes (with an addition of growth promoters or without) and an addition of dried green forage or dried herbs. The mechanical properties of the bones were determined on the basis of the three-point bend test performed with the use of an Instron 4302 apparatus coupled with a computer. The test registers as a graph the dependence between the force acting perpendicularly to the long axis of the bone and its deformation. An examination was performed to determine maximum force, and the maximum elasticity force. The studies revealed that both the type and the amount of the herbs determined chickens’ growth and the physical and strength properties of their bones. The most beneficial effects were observed in feeding birds mixtures containing 1% of pansy, since the values noted for all traits and indices were not worse than the effects obtained in the control group receiving antibiotic growth promoter, and in some cases they were even higher. The least positive results occurred in supplementing feed mixtures with 3% of nettle.
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