Wyniki wyszukiwania

1

The ghrelin pentapeptide inhibits the secretion of pancreatic juice in rats

100%

Kapica M., Laubitz D., Puzio I., Jankowska A., Zabielski R.

Journal of Physiology and Pharmacology

|

2006

|

tom 57

|

nr 4

Ghrelin, a 28 amino acids polypeptide was recognized as an endogenous ligand for the growth hormone secretagogue receptor. It turned out that the entire sequence of ghrelin is not necessary for performing the above-mentioned functions. It was suggested that 5 residues (Gly-Ser-Ser(n-octanoyl)-Phe, pentaghrelin) constituted functionally active part of the full-length polypeptide. Ghrelin-28 was found to inhibit pancreatic enzyme output in rats, though the effect of pentaghrelin was not studied so far. The study aimed to determine the involvement of pentaghrelin in pancreatic juice secretion in anaesthetized rats. Male Wistar rats (220 ± 20 g body weight, b. wt.) were anesthetized, the external jugular vein and common biliary-pancreatic duct were cannulated. Pentaghrelin boluses (iv, 1.2, 12, and 50 nmol kg-1 b. wt.) were injected every 30 min with or without CCK-8 infusion, duodenal mucosal CCK1 receptor blockade with tarazepide, vagotomy and capsaicin pretreatment. Pentaghrelin boluses reduced the volume of pancreatic-biliary juice, protein and trypsin outputs both under basal and CCK-8-stimulated conditions in a dose-dependent manner. However, exogenous pentaghrelin failed to affect the pancreatic secretion in rats subjected to vagotomy, capsaicin deactivation of afferents or pretreatment with Tarazepide. In conclusion, pentaghrelin may control exocrine pancreas secretion by affecting duodenal neurohormonal mechanism(s) involving CCK and vagal nerves in rats.

2

Physiological and chemical characteristics of antibacterial activity of pancreatic juice

100%

Laubitz D., Zabielski R., Wolinski J., Nieminuszczy J., Grzesiuk E.

Journal of Physiology and Pharmacology

|

2003

|

tom 54

|

nr 2

Attempts were made to find and characterize an antibacterial activity (ABA) factor in porcine pancreatic juice (PJ). Its isolation requires several steps. Since ABA factor was found to be heat resistant, the first step was heating for 30 min at 65°C. Afterwards column chromatography, ethanol precipitation and polyacrylamide gel electrophoresis were involved. Finally, we obtained a pancreatic juice fraction with antibacterial activity against Escherichia coli strain AB1157. In the presence of this fraction the number of living bacterial cells in overnight culture decreased about 10,000 fold and a spot-test gave clearly positive results. The results of analysis suggest that the antibacterial factor is a polypeptide active in a pH range 8.0 - 8.5, that migrates in polyacrylamide gel electrophoresis as a band under 14,000 Da. Mass spectroscopy analysis of active fraction showed high concentration of porcine pancreatic spasmolytic polypeptide (PSP). In conclusion, a polypeptide controlling bacterial homeostasis has been found in the porcine pancreatic juice.

3

Luminal CCK and its neuronal action on exocrine pancreatic secretion

84%

Zabielski R.

Journal of Physiology and Pharmacology. Supplement

|

2003

|

tom 54

|

nr 4

81-94

Gut regulatory peptides are produced by mucosal endocrine cells and released both into the circulation as well as into the gut lumen. Following stimulation the distribution between the circulation and gut lumen changes in favor of the gut lumen. In the blood plasma, the biological half-life of gut regulatory peptides is counted in single minutes due to high aminopeptidase activity and liver extraction. In the gut lumen, however, regulatory peptides retain their biological activity much longer, especially in newborn and young animals. A series of studies was performed in neonatal calves and pigs to explore the role of luminal cholecystokinin (CCK) on the regulation of exocrine pancreatic secretion. In anaesthetized neonatal calves, CCK was secreted into the duodenal lumen, and electrical vagal stimulation increased CCK release into the duodenal lumen but not into the circulating blood. In conscious calves, luminal CCK-8 stimulated pancreatic protein secretion by a neurohormonal mechanism dependent on a duodenal mucosal CCK1 receptor and vagal nerve activity. Immunocytochemistry pointed to an association of mucosal CCK1 and CCK2 receptors with neuronal components in the small intestine of neonatal calves. Experiments in calves and pigs with CCK-8 infusions into the duodenal branches of the right gastroepiploic artery confirmed the results of luminal CCK-8 and questioned the physiological relevance of a direct mechanism of CCK on the pancreatic acini.

4

Development and regulation of pancreatic juice secretion in cattle. State-of-the-art

84%

Zabielski R., Pierzynowski S. G.

Journal of Animal and Feed Sciences

|

2001

|

tom 10

|

nr 1

5

Inhibition of pancreatic juice secretion by M1 and M3 muscarinic receptor antagonists in conscious pigs

84%

Wolinski J., Yao G., Korczynski W., Biernat M., Babelewska M., Zabielski R.

Journal of Physiology and Pharmacology. Supplement

|

2001

|

tom 52

|

nr 2

6

The effect of exogenous apelin on the secretion of pancreatic juice in anaesthetized rats

67%

Kapica M., Jankowska A., Antushevich H., Pietrzak P., Bierla J. B., Dembinski A., Zabielski R.

Journal of Physiology and Pharmacology

|

2012

|

tom 63

|

nr 1

7

Intestinal MMC-related electric fields and pancreatic juice control the adhesion of gram-positive and gram-negative bacteria to the gut epithelium - in vitro study

67%

Jankowska A., Wrzesinski M., Laubitz D., Kazimierczak W., Skrzypek H., Bardowski J., Zabielski R., Grzesiuk E.

Journal of Physiology and Pharmacology

|

2008

|

tom 59

|

nr 4

795-810

The adhesion of six different Lactobacillus and Lactococcus and three pathogenic Escherichia and Salmonella strains was studied using Caco-2 cell line. In this in vitro model system the influence of weak electric field (EF) on bacterial adhesion was tested. The EF source was the in vitro reconstruction of spiking potentials recorded in the duodenum of a healthy calf during one myoelectrical migration complex (MMC) cycle. The ability to adhere to Caco-2 cells of bacteria belonging to two groups, Gram-positive lactobacilli and lactococci, and Gram-negative Escherichia and Salmonella differed considerably. The pathogenic bacteria adhered better to well-differentiated Caco-2 cells whereas lactobacilli and lactococci displayed better adhesion to non-differentiated Caco-2 cells. In the presence of MMC-related EF an increased adhesion of Lactobacillus and Lactococcus but not of Salmonella enterica s. Enteritidis and E. coli 269 to Caco-2 cells was observed. Two later strains adhered even less in the presence of EF. The same tendency was found in the presence of pancreatic juice in a cell medium. In conclusion, the myoelectric component of the small intestinal motility, the MMC-related EF, and pancreatic juice may increase the ability of lactic acid bacteria to adhere to GI epithelial cells, creating better environmental conditions for colonization of the intestine and competition with Gram-negative pathogens.

8

Obestatin stimulates the secretion of pancreatic juice enzymes through a vagal pathway in anaesthetized rats - preliminary results

67%

Kapica M., Zabielska M., Puzio I., Jankowska A., Kato I., Kuwahara A., Zabielski R.

Journal of Physiology and Pharmacology. Supplement

|

2007

|

tom 58

|

nr 3

123-130

9

Glutamate exocrine dynamics augmented by plasma glutamine and the distribution of amino acid transporters of the rat pancreas

67%

Fukushima D., Doi H., Fukushima K., Katsura K., Ogawa N., Sekiguchi S., Fujimori K., Sato A., Satomi S., Ishida K., Fukushima K.

Journal of Physiology and Pharmacology

|

2010

|

tom 61

|

nr 3

265-271

The nutritional and physiological roles of amino acid (AA)s have been investigated for individual organs. In the current study, we focused on the dynamics of glutamate and transport systems in the pancreas. We employed original procedures to obtain rat pancreatic juice (PJ) subjected to intravenous administration of alanyl-glutamine (AG) for AA analysis. The pancreatic expressions of the transporters were evaluated by immunohistochemistry. We found that glutamate was secreted into the PJ in the basal state. The intravenous administration of AG increased the concentration and total amount of glutamate excreted into the PJ. In terms of the transport systems, L-type AA transporter (LAT1) was identified exclusively in the islet cells. Glutamate transporter 1 (GLT1), glutamate-aspartate transporter (GLAST), vesicular glutamate transporter 1 (VGUT1) and cystine/glutamic acid transporter (xCT) were found in the islet cells. xCT was identified in the duct cells as well, but was not accompanied by the expression of 4F2 heavy chain (4F2hc) staining in the islets and the acinar cells, similar to neutral AA transporter (ASCT2) or b0,+-type AA transporter 1(BAT1). Excitatory AA transporter (EAAC) was identified only in the acinar cells. Glutamate was exclusively found in the acinar cells. We revealed the novel dynamics of glutamate in the rat PJ. The glutamate secretion into the PJ was augmented by plasma glutamine, indicating the de novo metabolisms of glutamate, together with the local expression of the related transporters.

10

Wydzielanie soku trzustkowego u swin po wykonanej roznymi metodami dlugotrwalej kaniulacji trzustki

67%

Winnicki T., Brzeski W.

Acta Academiae Agriculturae ac Technicae Olstenensis. Veterinaria

|

1995

|

tom 22

69-75

W pracy przedstawiono efekty uzyskane w rezultacie przeprowadzonej różnymi metodami długotrwałej kaniulacji trzustki u świń. Jedna z metod polegała na bezpośredniej kaniulacji przewodu trzustkowego dodatkowego po jego nacięciu, natomiast druga była metodą własną - kaniulę wprowadzano przez brodawkę dwunastniczą mniejszą. W czasie 14-dniowego okresu obserwacji oceniano skuteczność metod określając poziom wydzielania soku trzustkowego w obu grupach, różnice indywidualne wewnątrz grup oraz dynamikę wydzielania w ciągu doby. Korzystniejsze wyniki oraz większą efektywność wydzielania, stwierdzono w grupie zwierząt kaniulowanych poprzez brodawkę dwunastniczą mniejszą.

11

Wplyw zywienia pasza zawierajaca surowa i ekstrudowana maczke z nasion ledzwianu siewnego [Lathyrus sativus L.] na wydzielanie soku trzustkowego u cielat

51%

Szymanczyk S. E., Piedra J. L. V., Kapica M.

Acta Scientiarum Polonorum. Medicina Veterinaria

|

2007

|

tom 06

|

nr 4

47-61

Ograniczanie wyników

The ghrelin pentapeptide inhibits the secretion of pancreatic juice in rats

Physiological and chemical characteristics of antibacterial activity of pancreatic juice

Luminal CCK and its neuronal action on exocrine pancreatic secretion

Development and regulation of pancreatic juice secretion in cattle. State-of-the-art

Inhibition of pancreatic juice secretion by M1 and M3 muscarinic receptor antagonists in conscious pigs

The effect of exogenous apelin on the secretion of pancreatic juice in anaesthetized rats

Intestinal MMC-related electric fields and pancreatic juice control the adhesion of gram-positive and gram-negative bacteria to the gut epithelium - in vitro study

Obestatin stimulates the secretion of pancreatic juice enzymes through a vagal pathway in anaesthetized rats - preliminary results

Glutamate exocrine dynamics augmented by plasma glutamine and the distribution of amino acid transporters of the rat pancreas

Wydzielanie soku trzustkowego u swin po wykonanej roznymi metodami dlugotrwalej kaniulacji trzustki

Wplyw zywienia pasza zawierajaca surowa i ekstrudowana maczke z nasion ledzwianu siewnego [Lathyrus sativus L.] na wydzielanie soku trzustkowego u cielat