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1

Histomorphological and morphometric studies of the pancreatic islet cells of diabetic rats treated with extracts of Annona muricata

100%
Adeyemi D. O., Komolafe O. A., Adewole O. S., Obuotor E. M., Abiodun A. A., Adenowo T. K.
Folia Morphologica
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2010
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tom 69
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nr 2
Microanatomical changes in the pancreatic islet cells of streptozotocin induced diabetic Wistar rats were studied after treatment with methanolic extracts of Annona muricata leaves. Thirty adult Wistar rats were randomly assigned into three groups (control, untreated diabetic group, and A. muricata-treated diabetic group) of ten rats each. Diabetes mellitus was experimentally induced in groups B and C by a single intra-peritoneal injection of 80 mg/kg streptozotocin dissolved in 0.1 M citrate buffer. The control rats were intraperitoneally injected with an equivalent volume of citrate buffer. Daily intra peritoneal injections of 100 mg/kg A. muricata were administered to group C rats for two weeks. Post sacrifice the pancreases of the rats were excised and fixed in Bouin’s fluid. The tissues were processed for paraffin embedding and sections of 5 µm thickness were produced and stained with H & E, Gomori aldehyde fuchsin, and chrome alum haematoxylin-phloxine for demonstration of the β-cells of islets of pancreatic islets. Histomorphological and morphometric examination of the stained pancreatic sections showed a significant increase in the number, diameter, and volume of the β-cells of pancreatic islets of the A. muricata-treated group (5.67 ± 0.184 N/1000 µm², 5.38 ± 0.093 µm and 85.12 ± 4.24 µm³, respectively) when compared to that of the untreated diabetic group of rats (2.85 ± 0.361 N/1000 µm², 2.85 ± 0.362 µm and 69.56 ± 5.216 µm³, respectively). The results revealed regeneration of the β-cells of islets of pancreatic islet of rats treated with extract of A. muricata. (Folia Morphol 2010; 69, 2: 92–100)
2
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Increase of heat shock protein gene expression by melatonin in AR42J cells

100%
Bonior J., Jaworek J., Konturek S. J., Pawlik W. W.
Journal of Physiology and Pharmacology
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2005
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tom 56
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nr 3
Heat shock proteins (HSPs) have been reported to protect the pancreatic cells from the acute damage produced by caerulein overstimulation. However the effects of caerulein, melatonin or hyperthermia preconditioning on mRNA signal for HSP60 in the pancreatic acinar cells has not been examined yet. The aims of this study were: 1. To investigate the gene expression for HSP60 in the pancreatic AR42J cells stimulated by melatonin, caerulein or combination of both these substances. 2. To compare above changes with mRNA signal for HSP60 in pancreatic AR42J cells subjected to hyperthermia preconditioning. AR42J cells were incubated in standard medium at 37°C for: 0, 1, 3, 5, 12 or 24 h, under basal conditions. Above cells were then subjected to heat shock (42°C) for 0, 1 or 3 h. In the next part of the study AR42J cells were incubated in presence of caerulein (10-11, 10-9 or 10-7M), melatonin (10-8 or 10-6M), or combination of above under basal conditions or following heat shock pretreatment. Gene expression for HSP60 was determined by RT-PCR. The mRNA signal for HSP60 has been observed in AR42J cells under basal conditions, and this signal was markedly and time-dependently increased in these cells subjected to hyperthermia preconditioning. Incubation of AR42J cells in presence of melatonin (10-8 or 10-6M) resulted in the significant and dose-dependent increase of gene expression for HSP60 in both groups of AR42J cells: preconditioned and in those, which were not subjected to hyperthermia. Caerulein stimulation reduced mRNA signal for HSP60. The strongest signal has been observed after the exposition of AR42J cells to hyperthermia preconditioning, combined with melatonin and caerulein. We conclude that: 1. Gene expression for HSP60 has been detected in pancreatic AR42J cells under basal conditions. 2. Hyperthermia preconditioning resulted in a significant and time-dependent increase of HSP60 signal in pancreatic AR42J cells. 3. HSP60 gene expression was significantly increased in pancreatic AR42J cells stimulated by melatonin whereas caerulein reduced this signal. 4. The strongest gene expression for HSP60 has been found in the cells subjected to the combination of hyperthermia preconditioning, caerulein and melatonin.
3

Oxidative stress-dependent protein modification as a new signal for accelerated proteolysis in acute pancreatitis

100%
Sledzinski Z., Wozniak M., Kossowska E., Jankowski K., Stanek A., Wajda Z.
Journal of Physiology and Pharmacology. Supplement
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1998
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tom 49
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nr 2
4
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Alloxan stimulation and subsequent inhibition of insulin release from in situ perfused rat pancreas

80%
Kliber A., Szkudelski T., Chichlowska J.
Journal of Physiology and Pharmacology
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1996
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tom 47
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nr 2
5
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Phospholipase D mediated transphospatidylation as a possible new pathway of ethanol metabolism in isolated rat pancreatic acini

80%
Rydzewska G., Jurkowska G., Gabryelewicz A.
Journal of Physiology and Pharmacology
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1996
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tom 47
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nr 2
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