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Cloning and expression of two DNA fregments of the I-18 C region of Chironomus tentans. I. The scheme of the performed experiments and the applied technology

100%

Borowicz B. P.

Journal of Plant Protection Research

1998

tom 38

nr 1

The I-18 C region of the polytenic chromosome of Chironomus tentans contains the I-18 C gene. Two DNA fragments, reflecting two different open reading frames (i.e.ORF I and II) of two different transcripts (i.e. 1.8 and 4.6 kb RNA) of the I-18 C gene were isolated, then were cloned into the intermediate vector and next to the final vector in order to translate them in T7 RNA polymerase / promoter system. The translation of the ORF I and II was performed to obtain the polypeptides of these ORFs for further study. Here, the scheme of the performed experiments and the applied technology that were necessary to realize the goal of this research, are presented.

Ograniczanie wyników

1 Journal of Plant Protection Research

1 Borowicz B. P.

1 1998

Wyniki wyszukiwania

Cloning and expression of two DNA fregments of the I-18 C region of Chironomus tentans. I. The scheme of the performed experiments and the applied technology