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1

Activation and inhibition of ATM by phytochemicals: awakening and sleeping the guardian angel naturally

100%
Farooqi A. A., Wu S.-J., Chang Y.-T., Tang J.-Y., Li K.-T., Ismail M., Liaw C.-C., Li R.-N., Chang H. W.
Archivum Immunologiae et Therapiae Experimentalis
|
2015
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tom 63
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nr 5
2

Reactions of oxygen radicals with heme peroxidases

100%
Gebicka L., Gebicki J. L.
Current Topics in Biophysics
|
1994
|
tom 18
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nr 2
3
Content available remote

A novel, potent and selective inhibitor of the activity of inducible nitric oxide synthase [GW274150] reduces the organ injury in hemorrhagic shock

86%
McDonald M. C., Izumi M., Cuzzocrea S., Thiemermann C.
Journal of Physiology and Pharmacology
|
2002
|
tom 53
|
nr 4,1
An enhanced formation of nitric oxide (NO) by the inducible NO synfhase (iNOS) may contribute to the pathophysiology of hemorrhagic shock. This study investigates the effect of a novel, potent and selective inhibitor of iNOS activity (GW274150) on the circulatory failure and the organ injury and dysfunction associated with hemorrhagic shock in the anesthetised rat. Hemorrhage (sufficient to lower mean arterial blood pressure to 45 mmHg for 90 min) and subsequent resuscitation with shed blood resulted (within 4 h after resuscitation) in a delayed fall in blood pressure, renal and liver injury and dysfunction as well as the pancreatic injury. Pre-treatment of rats with GW274150 (5 mg/kg at 30 min prior to the onset of hemorrhage) attenuated the renal dysfunction as well as the liver and pancreatic injury caused by hemorrhage and resuscitation. Interestingly, GW274150 did not reduce the delayed fall in blood pressure associated with hemorrhagic shock. We propose that an enhanced formation of NO from iNOS contributes to the organ injury and dysfunction in hemorrhagic shock, and that highly selective inhibitors of iNOS activity, such as GW274150, may represent a novel therapeutic approach for the therapy of hemorrhagic shock.
4

BN52021 stabilizes the oxidant-antioxidant equilibrium in peritoneal lavage fluid in experimental hemorrhagic shock

86%
Debek W., Chyczewski L., Farbiszewski R.
Folia Histochemica et Cytobiologica
|
1999
|
tom 37
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nr 4
5

Scavenging of oxygen radicals by heme peroxidases

86%
Gebicka L., Gebicki J. L.
Acta Biochimica Polonica
|
1996
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tom 43
|
nr 4
The reactions of two heme peroxidases, horseradish peroxidase and lactoperoxidase and their compounds II (oxoferryl heme intermediates, Fe(IV)=0 or ferric protein radical Fe(III)R') and compounds III (resonance hybrids [Fe(IIIK)2 «-» Fe(II)-02l with superoxide radical anion generated enzymatically or radiolytically, and with hydroxyl radicals generated radiolytically, were investigated. It is suggested that only the protein radical form of compound II of lactoperoxidase reacts with superoxide, whereas compound II of horseradich peroxidase, which exists only in oxoferryl form, is unreactive towards superoxide. Compound III of the investigated peroxidases does not react with superoxide. The lactoperoxidase activity loss induced by hydroxyl radicals is closely related to the loss of the ability to form compound I (oxoferryl porphyrin n-cation radical, Fed V)=0(Por+) or oxoferryl protein radical Fe(IV)=0(R )). On the other hand, the modification of horseradish peroxidase induced by hydroxyl radicals has been reported to cause also restrictions in substrate binding (Gębicka, L. & Gębicki, J.L., 1996, Biochimie 78,62-65). Nevertheless, it has been found that only a small fraction of hydroxyl radicals generated homogeneously does inactivate the enzymes.
6
Content available remote

Effects of homogenization of induced sputum by dithiothreitol on polymorphonuclear cells

72%
Van Overveld F. J., Demkow U., Gorecka D., Skopinska-Rozewska E., De Backer W. A., Zielinski J.
Journal of Physiology and Pharmacology. Supplement
|
2005
|
tom 56
|
nr 4
143-154
Induced sputum represents a useful and non-invasive tool to isolate different cells from the airways. Complete homogenization of sputum is important for dispersion of cells and is usually achieved by use of dithiothreitol (DTT). However, it is not known if DTT will influence the viability and functionality of cells obtained by induced sputum. In the present study, induced sputum was processed by DTT or by PBS treatment. The obtained neutrophils were compared with neutrophils obtained from peripheral blood and from bronchoalveolar lavage fluid (BAL). These isolated neutrophils were treated in a similar way as the sputum neutrophils with DTT or PBS. All isolated cells were used for chemiluminescence tests and for the measurement of elastase and myeloperoxidase release after stimulation with fMLP. The results showed that the maximum chemiluminescence response was always significantly lower after DTT treatment: blood, 16.68 ±1.89 vs. 2.62 ±0.43 mV, P<0.0001; sputum, 2.96 ±0.30 vs. 1.09 ±0.01 mV, P<0.01; BAL, 25.47 ±0.88 vs. 8.22±0.20 mV, P<0.0001. Both spontaneous and fMLP-induced release of elastase and myeloperoxidase (MPO) was in most cases enhanced after DTT-treatment (P-values range from 0.24 to <0.01). We conclude that the use of DTT to homogenize sputum for dispersion of cells is harmful to cell functions and these cells are hampered for the evaluation of their normal functional characteristics.
7

Study on ischemic-reperfusion damage to the liver: hitherto existing successes and defeats

72%
Rutkowski T., Plewka A., Kaminski M.
Acta Poloniae Toxicologica
|
2000
|
tom 08
|
nr 1
8

Cerebrospinal fluid hipoxanthine, xanthine and uric acid concentrations in different inherited metabolic diseases [IMD]

72%
Larovere L., Latini A., Coronel C. E., De Kremer R. D.
Cellular and Molecular Biology Letters
|
1999
|
tom 04
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nr 3
9

Metabolic activation of adriamycin by NADPH-cytochrome P450 reductase; overview of its biological and biochemical effects

72%
Bartoszek A.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 2
NADPH-cytochrome P450 reductase (P450 reductase) is one of the enzymes impli­cated in the metabolism of adriamycin, a very important clinically used antitumour drug. However, apart from the enzyme involvement, so far little was known about the chemical route and biochemical effects of this process. We demonstrated that the ap­plication of P450 reductase simultaneously with adriamycin to tumour cells in culture significantly increased cytotoxicity of the drug. Under tissue culture conditions, we noticed also that, in the presence of P450 reductase, adriamycin metabolite(s), dis­playing an altered spectrum within the visible light range were formed. This observa­tion was taken adavantage of to study the metabolism of adriamycin in cell-free sys­tems, using initially the enzyme isolated from rat liver and the recently obtained re­combinant human P450 reductase. The reductive conversion of the drug turned out to be a multi-stage process, which occurred only under aerobic conditions and was ac­companied by excessive NADPH consumption. Further research carried out with the aid of radical scavengers and radiolabelled adriamycin revealed that the enhance­ment of biological activity of adriamycin by P450 reductase stemmed from the forma­tion of alkylating metabolite(s) rather than from the promotion of redox cycling known to be induced in the presence of anthracyclines.
10

The effect of Fd-NADP oxido-reductase on oxidation of SO2

72%
Miszalski Z., Niewiadomska E.
Bulletin of the Polish Academy of Sciences. Biological Sciences
|
1992
|
tom 40
|
nr 4
11
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Content available

An overall view of the process of the regulation of human iron metabolism

58%
Formanowicz D., Formanowicz P.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2011
|
tom 92
|
nr 2
12

Vaccination of rats against the rodent hookworm Nippostrongylus brasiliensis with a recombinant superoxide dismutase fails to protect against infection

58%
Ball G., Knox D. P.
Acta Parasitologica
|
2009
|
tom 54
|
nr 3
281-287
Anti-oxidant enzymes including superoxide dismutase (SOD) protect cells from damage by oxygen radicals produced during respiration. There is also a substantial body of evidence that anti-oxidant enzymes facilitate the survival of parasitic helminths, including gastrointestinal nematodes, within the host. Superoxide dismutase has been shown to be released by a variety of parasitic helminths and may protect them from host mediated oxidative immune responses. As it may play a parasite protective role during infections SOD has been investigated as a vaccine candidate in a range of helminth parasites including Schistosoma mansoni, Acanthocheilonema viteae and Haemonchus contortus. Here, we sought to evaluate the protective potential of SOD against the rat hookworm Nippostrongylus brasiliensis, a commonly utilised laboratory infection, as a vaccination model. A cytosolic SOD from this parasite, with high sequence homology to a putative extracellular form of the enzyme was cloned and then expressed in bacteria. The resultant recombinant protein was assessed for enzyme activity and used to immunise rats prior to a single challenge infection with the parasite. No protection was observed and monitoring systemic and mucosal antibody responses and mast cell protease levels in superoxide dismutase vaccinated rats suggested that this recombinant SOD was only weakly immunogenic.
13

Protein kinase C isoforms in neutrophil adhesion and activation

58%
Bertram A., Ley K.
Archivum Immunologiae et Therapiae Experimentalis
|
2011
|
tom 59
|
nr 2
14

Peroxidase activity, ascorbate and oxygen radicals contents in tomato apoplast fractions after Botrytis cinerea infection

58%
Patykowski J., Cicha A.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
15

Antioxidative factors in lipid metabolism

58%
Ziemlanski S., Wartanowicz M., Panczenko-Kresowska B.
Polish Journal of Food and Nutrition Sciences
|
1998
|
tom 07
|
nr 2
16
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Role of free radicals in septic shock

58%
Novelli G. P.
Journal of Physiology and Pharmacology
|
1997
|
tom 48
|
nr 4
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