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The aim of the study was to estimate nutritional and physiological consequences of combined supplementation of rat casein diets with a strongly oxidized mixture of fats and extract of polyphenols from chokeberry. The experiment was conducted on 80 young Wistar rats divided into 8 groups. The animals were fed diets supplemented with 8% of fresh or oxidized fats and 0.4, 0.8 and 1.2% of polyphenol extract together. Addition of the oxidized fats caused significant reduction in animal growth without a decrease in nitrogen digestibility. On the other hand, the extract of polyphenols suppressed nitrogen utilization; animal growth was however similar to the control group. Analysis of the variance showed negative influence of the oxidized fats on cholesterol and triacyloglycerol concentration in the blood of the experimental animals. Increased activity of both aminotransferases in the blood of rats fed diets containing the oxidized fats indicated on liver malfunction. There was no effect of the experimental factors on the antioxidant status of the blood, but a dose-dependent reduced content of malonylodialdehyde in the blood of rats fed diets supplemented with the extract of polyphenols suggested that the extract had a preventive effect against prooxidative activity of the oxidized fats.
Microscopic analyses investigated specimens of internal organs and muscles of 20 BUT-9 turkeys fed for 16 weeks with mixtures supplemented with fat of varying peroxide values: as high as 5, 50 100 and 150 mEq O2/kg. During the 4-week experiment period 2, 3, 4 and 5% fat (rapeseed oil and poultry fat at a proportion of 66:34 oxidized under controlled conditions) was added respectively to the feed. Supplementing the oxidized fat at PV 5 mEq O2/kg did not cause any morphological lesions in the internal organs of the turkey hens. However, an increase of up to 50 mEq O2/kg in the fat peroxide value of their diet resulted in retrogressive changes and disturbances in the blood supply to the liver and cross striated muscles. Fat at PV 100 mEq O2/kg resulted in a slight intensification of the above-mentioned changes mainly in these organs, however, fat oxidation of 150 mEq O2/kg led to a visible increase in the type and intensity of lesions as well as the areas in which they occurred (kidneys and alimentary tract). The observed morphological defects were lesions of a damaging and adaptive profile.
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